EC 3.1.2.12: S-formylglutathione hydrolase
HydrolasesOpen in Browse
CatLog read 63 records for EC 3.1.2.12 from 4 papers, for 3 organisms. Names in these papers: S-formylglutathione hydrolase (ySFGH), S-formylglutathione hydrolase (SFGH), S-formylglutathione hydrolase YeiG, S-formylglutathione hydrolase AtuSFGH (Atu1476) and 1 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 12 records for EC 3.1.2.12 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.1.2.12 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Structural characterization and reversal of the natural organophosphate resistance of a D-type esterase, Saccharomyces cerevisiae S-formylglutathione hydrolase | Saccharomyces cerevisiae | 25 |
| Molecular basis of formaldehyde detoxification. Characterization of two S-formylglutathione hydrolases from Escherichia coli, FrmB and YeiG | Escherichia coli | 17 |
| A role for His-160 in peroxide inhibition of S. cerevisiae S-formylglutathione hydrolase: evidence for an oxidation sensitive motif | Saccharomyces cerevisiae | 15 |
| The structure of a putative S-formylglutathione hydrolase from Agrobacterium tumefaciens | Agrobacterium tumefaciens | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| S-formylglutathione hydrolase (SFGH)variant C60A | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.98 | 0.19 | 5.2 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant C60A, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetate | — | 2.5 | — | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.85 | — | — | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetate | — | — | 0.33 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | — | 3.5 | — | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | 3.2 | — | — | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | — | — | 0.83 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | — | 2.6 | — | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | 2.8 | — | — | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | — | — | 1.2 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160S | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.48 | 0.16 | 2.8 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant H160S, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant N64A | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 1.1 | 0.78 | 1.42 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant N64A, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant W197I/H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.048 | 0.99 | 0.05 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant W197I/H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant W197I/H160I | Saccharomyces cerevisiae | S-lactoylglutathione | 0.118 | 0.9 | 0.13 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant W197I/H160I, S-lactoylglutathione |
| S-formylglutathione hydrolase (SFGH)variant Y278F | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.6 | 0.18 | 3.3 | pH 7.4, 22 °C | PMID 22906720 | Open record: S-formylglutathione hydrolase (SFGH) variant Y278F, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH) | Saccharomyces cerevisiae | p-nitrophenyl acetateSorensen's buffer (0.067 M Na/K phosphate); 22 ± 2 °C | 1.8 | 0.4 | 4.5 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH), p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH) | Saccharomyces cerevisiae | p-nitrophenyl acetateSorensen's buffer; 22 °C; absorbance at 405 nm | 1.78 | 0.5 | 3.6 calc. | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH), p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH) | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.198 | 0.09 | 2.2 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH), p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60H/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 1.5 | 0.34 | 4.3 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60H/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60H/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 3.63 | 0.13 | 28 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60H/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60K/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.972 | 0.15 | 6.5 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60K/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60K/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 1.23 | 0.03 | 42 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60K/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60Q/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.525 | 0.1 | 5.3 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60Q/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60Q/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 1.65 | 0.012 | 130 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60Q/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60R/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.317 | 0.013 | 25 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60R/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60R/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.43 | 0.018 | 23 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60R/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60S | Saccharomyces cerevisiae | p-nitrophenyl acetate | 1.58 | 0.32 | 5 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60S | Saccharomyces cerevisiae | p-nitrophenyl acetateenzyme stock with 2-4 mM bME diluted 1:100 | 1.53 | 0.19 | 8 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60S | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.078 | 0.09 | 0.83 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60S/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.763 | 0.14 | 5.5 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant C60S/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 1.97 | 0.019 | 103 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant C60S/W197I | Saccharomyces cerevisiae | S-lactoylglutathione | 50 | 3 | 17 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant C60S/W197I, S-lactoylglutathione |
| S-formylglutathione hydrolase (ySFGH)variant L58H/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.0055 | 0.23 | 0.025 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant L58H/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant M162H | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.1 | 0.9 | 0.12 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant M162H, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant M162H | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.0032 | 0.14 | 0.023 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant M162H, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant M162H/C60S/W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 0.028 | 2.6 | 0.012 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant M162H/C60S/W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant M162H/C60S/W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 0.04 | 0.12 | 0.33 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant M162H/C60S/W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant W197I | Saccharomyces cerevisiae | p-nitrophenyl acetate | 1.05 | 0.054 | 20 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant W197I, p-nitrophenyl acetate |
| S-formylglutathione hydrolase (ySFGH)variant W197I | Saccharomyces cerevisiae | p-nitrophenyl butyrate | 1.92 | 0.04 | 48 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant W197I, p-nitrophenyl butyrate |
| S-formylglutathione hydrolase (ySFGH)variant W197I | Saccharomyces cerevisiae | S-lactoylglutathione | 120 | 3 | 33 | pH 7.4, 22 °C | PMID 18707125 | Open record: S-formylglutathione hydrolase (ySFGH) variant W197I, S-lactoylglutathione |
| S-formylglutathione hydrolase AtuSFGH (Atu1476) | Agrobacterium tumefaciens | 1-naphthyl butyrate | 1.73 | 0.44 | 3.93 | pH 8 | PMID 19653299 | Open record: S-formylglutathione hydrolase AtuSFGH (Atu1476), 1-naphthyl butyrate |
| S-formylglutathione hydrolase AtuSFGH (Atu1476) | Agrobacterium tumefaciens | 1-naphthyl propionate | 1.9 | 0.35 | 5.54 | pH 8 | PMID 19653299 | Open record: S-formylglutathione hydrolase AtuSFGH (Atu1476), 1-naphthyl propionate |
| S-formylglutathione hydrolase AtuSFGH (Atu1476) | Agrobacterium tumefaciens | 4-nitrophenyl acetate | 1.95 | 0.48 | 4.07 | pH 8 | PMID 19653299 | Open record: S-formylglutathione hydrolase AtuSFGH (Atu1476), 4-nitrophenyl acetate |
| S-formylglutathione hydrolase AtuSFGH (Atu1476) | Agrobacterium tumefaciens | 4-nitrophenyl butyrate | 4.48 | 0.11 | 40.8 | pH 8 | PMID 19653299 | Open record: S-formylglutathione hydrolase AtuSFGH (Atu1476), 4-nitrophenyl butyrate |
| S-formylglutathione hydrolase AtuSFGH (Atu1476) | Agrobacterium tumefaciens | 4-nitrophenyl hexanoate | 0.67 | 0.02 | 39.6 | pH 8 | PMID 19653299 | Open record: S-formylglutathione hydrolase AtuSFGH (Atu1476), 4-nitrophenyl hexanoate |
| S-formylglutathione hydrolase AtuSFGH (Atu1476) | Agrobacterium tumefaciens | 4-nitrophenyl propionate | 1.84 | 0.05 | 38.5 | pH 8 | PMID 19653299 | Open record: S-formylglutathione hydrolase AtuSFGH (Atu1476), 4-nitrophenyl propionate |
| S-formylglutathione hydrolase FrmB | Escherichia coli | 4-nitrophenyl acetate | 0.3 | 0.29 | 1.03 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase FrmB, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase FrmB | Escherichia coli | 4-nitrophenyl propionate | 0.14 | 0.83 | 0.17 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase FrmB, 4-nitrophenyl propionate |
| S-formylglutathione hydrolase FrmB | Escherichia coli | S-formylglutathione | 28.5 | — | — | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase FrmB, S-formylglutathione |
| S-formylglutathione hydrolase FrmB | Escherichia coli | S-lactoylglutathione | 0.05 | 0.6 | 0.08 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase FrmB, S-lactoylglutathione |
| S-formylglutathione hydrolase YeiG | Escherichia coli | 1-naphthyl acetate | 5.09 | 1.03 | 4.94 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG, 1-naphthyl acetate |
| S-formylglutathione hydrolase YeiG | Escherichia coli | 4-nitrophenyl acetate | 0.26 | 0.45 | 0.57 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase YeiG | Escherichia coli | 4-nitrophenyl butyrate | 0.36 | 0.7 | 0.51 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG, 4-nitrophenyl butyrate |
| S-formylglutathione hydrolase YeiG | Escherichia coli | 4-nitrophenyl hexanoate | 0.49 | 0.95 | 0.52 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG, 4-nitrophenyl hexanoate |
| S-formylglutathione hydrolase YeiG | Escherichia coli | 4-nitrophenyl propionate | 0.37 | 0.48 | 0.76 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG, 4-nitrophenyl propionate |
| S-formylglutathione hydrolase YeiG | Escherichia coli | S-formylglutathione | 6.51 | — | — | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG, S-formylglutathione |
| S-formylglutathione hydrolase YeiG | Escherichia coli | S-lactoylglutathione | 1.02 | 0.58 | 1.75 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG, S-lactoylglutathione |
| S-formylglutathione hydrolase YeiGvariant C26A | Escherichia coli | 1-naphthyl acetate | 5.12 | 1.06 | 3.2 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG variant C26A, 1-naphthyl acetate |
| S-formylglutathione hydrolase YeiGvariant C54A | Escherichia coli | 1-naphthyl acetate | 5.1 | 1.28 | 3.98 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG variant C54A, 1-naphthyl acetate |
| S-formylglutathione hydrolase YeiGvariant D199A | Escherichia coli | 1-naphthyl acetate | 2.96 | 1.01 | 2.93 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG variant D199A, 1-naphthyl acetate |
| S-formylglutathione hydrolase YeiGvariant D218A | Escherichia coli | 1-naphthyl acetate | 3.81 | 1.3 | 2.93 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG variant D218A, 1-naphthyl acetate |
| S-formylglutathione hydrolase YeiGvariant D255A | Escherichia coli | 1-naphthyl acetate | 2.23 | 0.54 | 4.13 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG variant D255A, 1-naphthyl acetate |
| S-formylglutathione hydrolase YeiGvariant D80A | Escherichia coli | 1-naphthyl acetate | 2.17 | 0.8 | 2.71 | 37 °C | PMID 16567800 | Open record: S-formylglutathione hydrolase YeiG variant D80A, 1-naphthyl acetate |