Values CatLog read from this paper
A role for His-160 in peroxide inhibition of S. cerevisiae S-formylglutathione hydrolase: evidence for an oxidation sensitive motif
PMID 22906720doi:10.1016/j.abb.2012.08.001Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (27 values) from this paper: S-formylglutathione hydrolase (SFGH) from Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| S-formylglutathione hydrolase (SFGH)variant C60A | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.98 | 0.19 | 5.2 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant C60A, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetate | — | 2.5 | — | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.85 | — | — | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetate | — | — | 0.33 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | — | 3.5 | — | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | 3.2 | — | — | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | — | — | 0.83 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | — | 2.6 | — | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | 2.8 | — | — | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetateH160I-ox: enzyme preincubated 1 min with 5 mM pNP-acetate | — | — | 1.2 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant H160S | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.48 | 0.16 | 2.8 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant H160S, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant N64A | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 1.1 | 0.78 | 1.42 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant N64A, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant W197I/H160I | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.048 | 0.99 | 0.05 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant W197I/H160I, 4-nitrophenyl acetate |
| S-formylglutathione hydrolase (SFGH)variant W197I/H160I | Saccharomyces cerevisiae | S-lactoylglutathione | 0.118 | 0.9 | 0.13 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant W197I/H160I, S-lactoylglutathione |
| S-formylglutathione hydrolase (SFGH)variant Y278F | Saccharomyces cerevisiae | 4-nitrophenyl acetate | 0.6 | 0.18 | 3.3 | pH 7.4, 22 °C | Open record: S-formylglutathione hydrolase (SFGH) variant Y278F, 4-nitrophenyl acetate |