Values CatLog read from this paper
Histidine 55 of tryptophan 2,3-dioxygenase is not an active site base but regulates catalysis by controlling substrate binding
PMID 18783250doi:10.1021/bi801202aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 34 records (56 values) from this paper: tryptophan 2,3-dioxygenase from Xanthomonas campestris.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 22 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | 5-methyl-DL-tryptophan | 3.59 | 0.357 | 10.1 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase, 5-methyl-DL-tryptophan |
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | 6-methyl-DL-tryptophan | 41 | 0.975 | 42 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase, 6-methyl-DL-tryptophan |
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | L-tryptophan | 19.7 | 0.256 | 77.0 calc. | pH 6, 25 °C | Open record: tryptophan 2,3-dioxygenase, L-tryptophan |
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | L-tryptophanapparent kcat | 21.7 | — | — | 25 °C | Open record: tryptophan 2,3-dioxygenase, L-tryptophan |
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | L-tryptophan | — | 0.162 | — | 25 °C | Open record: tryptophan 2,3-dioxygenase, L-tryptophan |
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | L-tryptophanapparent kcat | 19 | — | — | 25 °C | Open record: tryptophan 2,3-dioxygenase, L-tryptophan |
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | L-tryptophan | — | 0.089 | — | 25 °C | Open record: tryptophan 2,3-dioxygenase, L-tryptophan |
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | L-tryptophan | 21.6 | 0.121 | 179 calc. | pH 7, 25 °C | Open record: tryptophan 2,3-dioxygenase, L-tryptophan |
| tryptophan 2,3-dioxygenase | Xanthomonas campestris | L-tryptophan | 18.3 | 0.0912 | 201 calc. | pH 8, 25 °C | Open record: tryptophan 2,3-dioxygenase, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | 5-fluoro-DL-tryptophan | 0.68 | 0.194 | 3.5 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, 5-fluoro-DL-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | 5-methyl-DL-tryptophan | 0.4 | 0.395 | 1.0 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, 5-methyl-DL-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | 6-fluoro-DL-tryptophan | 3.78 | 0.195 | 19.4 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, 6-fluoro-DL-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | 6-methyl-DL-tryptophan | 1.65 | 0.386 | 4.27 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, 6-methyl-DL-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | dioxygen | 3.86 | 0.0457 | 84.5 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, dioxygen |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | L-tryptophan | 5.86 | 0.136 | 43.1 calc. | pH 6, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | L-tryptophanapparent kcat | 5.42 | — | — | 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | L-tryptophan | — | 0.166 | — | 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | L-tryptophanapparent kcat | 2.86 | — | — | 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | L-tryptophan | — | 0.218 | — | 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | L-tryptophan | 2.7 | 0.164 | 17 calc. | pH 8, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55A | Xanthomonas campestris | L-tryptophan | 4.69 | 0.213 | 22.0 calc. | pH 7, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55A, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | 5-fluoro-DL-tryptophan | 0.8 | 0.183 | 4.4 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, 5-fluoro-DL-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | 5-methyl-DL-tryptophan | 1.45 | 1.302 | 1.11 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, 5-methyl-DL-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | 6-fluoro-DL-tryptophan | 3.8 | 0.546 | 7.0 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, 6-fluoro-DL-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | 6-methyl-DL-tryptophan | 4.66 | 1.98 | 2.35 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, 6-methyl-DL-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | dioxygen | 7.88 | 0.099 | 80 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, dioxygen |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | L-tryptophan | 2.6 | 0.197 | 13 calc. | pH 7.5, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | L-tryptophan | 4.91 | 0.3 | 16 calc. | pH 6, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | L-tryptophanapparent kcat | 3.66 | — | — | 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | L-tryptophan | — | 0.23 | — | 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | L-tryptophanapparent kcat | 2.1 | — | — | 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | L-tryptophan | — | 0.121 | — | 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | L-tryptophan | 3.09 | 0.192 | 16.1 calc. | pH 7, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, L-tryptophan |
| tryptophan 2,3-dioxygenasevariant H55S | Xanthomonas campestris | L-tryptophan | 2.28 | 0.134 | 17.0 calc. | pH 8, 25 °C | Open record: tryptophan 2,3-dioxygenase variant H55S, L-tryptophan |