Values CatLog read from this paper

Random mutagenesis improves the low-temperature activity of the tetrameric 3-isopropylmalate dehydrogenase from the hyperthermophile Sulfolobus tokodaii

PMID 18854331doi:10.1093/protein/gzn054Open in Browse

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CatLog read 36 records (78 values) from this paper: 3-isopropylmalate dehydrogenase from Sulfolobus tokodaii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.85

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
3-isopropylmalate dehydrogenase	Sulfolobus tokodaii	D-3-isopropylmalate	0.15	—	—	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase, D-3-isopropylmalate
3-isopropylmalate dehydrogenase	Sulfolobus tokodaii	D-3-isopropylmalate	0.7	0.0042	170	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase, D-3-isopropylmalate
3-isopropylmalate dehydrogenase	Sulfolobus tokodaii	D-3-isopropylmalate	6.9	0.0055	1.3 × 10³	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase, D-3-isopropylmalate
3-isopropylmalate dehydrogenase	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.24	0.62	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenase	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.26	2.7	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenase	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.39	18	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S	Sulfolobus tokodaii	D-3-isopropylmalate	0.16	—	—	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S	Sulfolobus tokodaii	D-3-isopropylmalate	0.79	0.0035	230	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S	Sulfolobus tokodaii	D-3-isopropylmalate	6.8	0.0076	900	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.15	1.1	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.27	2.9	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.39	18	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S/L288P	Sulfolobus tokodaii	D-3-isopropylmalate	0.17	—	—	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/L288P, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S/L288P	Sulfolobus tokodaii	D-3-isopropylmalate	0.9	0.0046	200	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/L288P, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S/L288P	Sulfolobus tokodaii	D-3-isopropylmalate	7.8	0.0067	1.2 × 10³	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/L288P, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S/L288P	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.21	0.8	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/L288P, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S/L288P	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.21	4.3	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/L288P, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S/L288P	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.4	20	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/L288P, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S/S232G	Sulfolobus tokodaii	D-3-isopropylmalate	0.1	—	—	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/S232G, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S/S232G	Sulfolobus tokodaii	D-3-isopropylmalate	0.55	0.0025	220	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/S232G, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S/S232G	Sulfolobus tokodaii	D-3-isopropylmalate	3.8	0.0039	1 × 10³	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/S232G, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant F3S/S232G	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.1	0.99	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/S232G, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S/S232G	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.1	5.4	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/S232G, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant F3S/S232G	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.22	17	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant F3S/S232G, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant L288P	Sulfolobus tokodaii	D-3-isopropylmalate	0.18	—	—	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant L288P, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant L288P	Sulfolobus tokodaii	D-3-isopropylmalate	0.81	0.0026	310	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant L288P, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant L288P	Sulfolobus tokodaii	D-3-isopropylmalate	7.5	0.0044	1.7 × 10³	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant L288P, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant L288P	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.18	1	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant L288P, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant L288P	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.19	4.4	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant L288P, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant L288P	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.37	20	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant L288P, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant R284G	Sulfolobus tokodaii	D-3-isopropylmalate	0.18	—	—	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant R284G, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant R284G	Sulfolobus tokodaii	D-3-isopropylmalate	0.81	0.0053	150	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant R284G, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant R284G	Sulfolobus tokodaii	D-3-isopropylmalate	6	0.008	750	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant R284G, D-3-isopropylmalate
3-isopropylmalate dehydrogenasevariant R284G	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.18	1	pH 7.5, 25 °C	Open record: 3-isopropylmalate dehydrogenase variant R284G, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant R284G	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.22	3.7	pH 7.5, 40 °C	Open record: 3-isopropylmalate dehydrogenase variant R284G, nicotinamide adenine dinucleotide
3-isopropylmalate dehydrogenasevariant R284G	Sulfolobus tokodaii	nicotinamide adenine dinucleotide	—	0.25	24	pH 7.5, 70 °C	Open record: 3-isopropylmalate dehydrogenase variant R284G, nicotinamide adenine dinucleotide