CatLogenzyme kinetics catalog

Values CatLog read from this paper

Hydrogen bond residue positioning in the 599-611 loop of thimet oligopeptidase is required for substrate selection

PMID 18959747doi:10.1111/j.1742-4658.2008.06685.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 34 records (102 values) from this paper: thimet oligopeptidase (EP24.15) from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.4.24.15

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
thimet oligopeptidase (EP24.15)Rattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.35.7 × 10⁻⁵5.9 × 10³pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)Rattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol0.440.0078850pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)Rattus norvegicusMca-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-OH11.20.024470pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)Rattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)0.330.0012280pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G599ARattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.861.29 × 10⁻⁴6.7 × 10³pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G599ARattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol0.030.004087pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G599ARattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)0.180.002570pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603ARattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.285.4 × 10⁻⁵5.2 × 10³pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603ARattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol2.320.00401580pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603ARattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)1.260.0029430pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603A/G604ARattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.274.1 × 10⁻⁵6.59 × 10³pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603A/G604ARattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol2.440.00543450pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603A/G604ARattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)1.220.0057210pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603PRattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.180.002190pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603PRattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol0.00260.006430.41pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G603PRattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)0.004490.00291.6pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G604ARattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.539 × 10⁻⁵5.8 × 10³pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G604ARattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol0.110.0082513pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G604ARattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)0.110.002640pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G611ARattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin1.341.36 × 10⁻⁴9.8 × 10³pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G611ARattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol0.1930.006729pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant G611ARattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)0.30.00560pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y605FRattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.348 × 10⁻⁵4.4 × 10³pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y605FRattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol0.00860.00821.1pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y605FRattus norvegicusMca-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-OH1.40.03738pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y605FRattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)0.0120.00422.9pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y605F/Y612FRattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.15.1 × 10⁻⁴200pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y605F/Y612FRattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol0.00230.00810.28pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y605F/Y612FRattus norvegicusMca-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-OH0.0250.0141.7pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y605F/Y612FRattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)0.00150.00310.47pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y612FRattus norvegicus(7-methoxycoumarin-4-acetyl)-[Ala7, Lys(dinitrophenol)9]-bradykinin0.755.7 × 10⁻⁴1.3 × 10³pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y612FRattus norvegicus7-methoxycoumarin-4-acetyl-Pro-Leu-Gly-Pro-Lys-dinitrophenol3.7 × 10⁻⁴0.0090.041pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y612FRattus norvegicusMca-Glu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-OH0.0610.0341.8pH 7.8, 23 °COpen
thimet oligopeptidase (EP24.15)variant Y612FRattus norvegicusMca-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Lys(Dnp)-OH (modified neurotensin)5.2 × 10⁻⁴0.00260.2pH 7.8, 23 °COpen