CatLogenzyme kinetics catalog

Values CatLog read from this paper

Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins

PMID 18975973doi:10.1021/bi8016119Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (12 values) from this paper: Dbp3p, Dbp4p, Rok1p and 1 more from Saccharomyces cerevisiae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.6.4.13

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
Dbp3pSaccharomyces cerevisiaeATPRNA-dependent ATPase; 25 mM KCl; coupled assay: 200 µM NADH2.831.32.2 calc.pH 7.5, 25 °COpen
Dbp3pSaccharomyces cerevisiaeATPRNA-dependent ATPase at high ionic strength: 110 mM KCl—1.7—pH 7.5, 25 °COpen
Dbp4pSaccharomyces cerevisiaeATPRNA-dependent ATPase; 25 mM KCl; coupled assay: 200 µM NADH0.830.243.5 calc.pH 7.5, 25 °COpen
Dbp4pSaccharomyces cerevisiaeATPRNA-dependent ATPase at high ionic strength: 110 mM KCl—0.39—pH 7.5, 25 °COpen
Rok1pSaccharomyces cerevisiaeATPRNA-dependent ATPase; 25 mM KCl; coupled assay: 200 µM NADH0.251.10.23 calc.pH 6.5, 25 °COpen
Rok1pSaccharomyces cerevisiaeATPRNA-dependent ATPase at high ionic strength: 110 mM KCl—1.6—pH 6.5, 25 °COpen
Rrp3pSaccharomyces cerevisiaeATPRNA-dependent ATPase; 25 mM KCl; coupled assay: 200 µM NADH0.222.30.094 calc.pH 7.5, 25 °COpen
Rrp3pSaccharomyces cerevisiaeATPRNA-dependent ATPase at high ionic strength: 110 mM KCl—2.8—pH 7.5, 25 °COpen