Values CatLog read from this paper
The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase
PMID 19019081doi:10.1111/j.1742-4658.2008.06761.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (20 values) from this paper: serine hydroxymethyltransferase (eSHMT) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.00703 | — | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT), (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine | 11.44 | 0.14 | 82 calc. | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-threo-3-phenylserine | 3.368 | 36.3 | 0.0928 calc. | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-threo-3-phenylserine |
| serine hydroxymethyltransferase (eSHMT)variant L276A | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.00435 | — | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L276A, (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferase (eSHMT)variant L276A | Escherichia coli | L-serine | 6.675 | 0.2 | 33 calc. | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L276A, L-serine |
| serine hydroxymethyltransferase (eSHMT)variant L276A | Escherichia coli | L-threo-3-phenylserine | 2.205 | 42.6 | 0.0518 calc. | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L276A, L-threo-3-phenylserine |
| serine hydroxymethyltransferase (eSHMT)variant L85A | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.00716 | — | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L85A, (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferase (eSHMT)variant L85A | Escherichia coli | L-serine | 10.79 | 0.15 | 72 calc. | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L85A, L-serine |
| serine hydroxymethyltransferase (eSHMT)variant L85A | Escherichia coli | L-threo-3-phenylserine | 4.297 | 37.4 | 0.115 calc. | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L85A, L-threo-3-phenylserine |
| serine hydroxymethyltransferase (eSHMT)variant L85A/L276A | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.0112 | — | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L85A/L276A, (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferase (eSHMT)variant L85A/L276A | Escherichia coli | L-serine | 6.67 | 0.2 | 33 calc. | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L85A/L276A, L-serine |
| serine hydroxymethyltransferase (eSHMT)variant L85A/L276A | Escherichia coli | L-threo-3-phenylserine | 2.898 | 42.1 | 0.0688 calc. | pH 7.2, 20 °C | Open record: serine hydroxymethyltransferase (eSHMT) variant L85A/L276A, L-threo-3-phenylserine |