EC 2.1.2.1: glycine hydroxymethyltransferase

TransferasesOpen in Browse

CatLog read 241 records for EC 2.1.2.1 from 15 papers, for 10 organisms. Names in these papers: serine hydroxymethyltransferase (eSHMT), serine hydroxymethyltransferase (AhSHMT), SHMT2 (mitochondrial serine hydroxymethyltransferase), SHMT1 (cytosolic serine hydroxymethyltransferase) and 13 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 78 records for EC 2.1.2.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.1.2.1 in Browse.

Papers

Papers with records for EC 2.1.2.1
Paper	Organisms	Records
Structural and kinetic properties of serine hydroxymethyltransferase from the halophytic cyanobacterium Aphanothece halophytica provide a rationale for salt tolerance	Aphanothece halophytica, Escherichia coli	70
Human Cytosolic and Mitochondrial Serine Hydroxymethyltransferase Isoforms in Comparison: Full Kinetic Characterization and Substrate Inhibition Properties	Homo sapiens	45
Human and Plasmodium serine hydroxymethyltransferases differ in rate-limiting steps and pH-dependent substrate inhibition behavior	Homo sapiens, Plasmodium vivax	21
Structural basis of methotrexate and pemetrexed action on serine hydroxymethyltransferases revealed using plant models	Arabidopsis thaliana	20
Role of a conserved active site cation-pi interaction in Escherichia coli serine hydroxymethyltransferase	Escherichia coli	14
The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase	Escherichia coli	12
Role of proline residues in the folding of serine hydroxymethyltransferase	Escherichia coli	9
Serine hydroxymethyltransferase from Plasmodium vivax is different in substrate specificity from its homologues	Plasmodium vivax	8
One-carbon metabolism in plants: characterization of a plastid serine hydroxymethyltransferase	Arabidopsis thaliana	8
Characterization of Plasmodium falciparum serine hydroxymethyltransferase-A potential antimalarial target	Plasmodium falciparum	7
Serine hydroxymethyltransferase from the silkworm Bombyx mori: Identification, distribution, and biochemical characterization	Bombyx mori	7
Overexpression and characterization of dimeric and tetrameric forms of recombinant serine hydroxymethyltransferase from Bacillus stearothermophilus	Bacillus stearothermophilus, Ovis aries (sheep)	6
Distinct biochemical properties of human serine hydroxymethyltransferase compared with the Plasmodium enzyme: implications for selective inhibition	Homo sapiens	6
Oligomeric defects in soybean serine hydroxymethyltransferase 8: tetramer destabilization by A149T and other variants associated with soybean cyst nematode resistance	Glycine max	6
How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state	Homo sapiens	2

Records

Records for EC 2.1.2.1 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	11.65	0.45	pH 6.5, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	8.05	1.01	pH 7, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	2.86	4.53	pH 8, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	1.78	11.57	pH 8.5, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	0.71	30.73	pH 9.5, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	5.3	0.0836	60	pH 6.5, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	8.1	0.1536	50	pH 7, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	13	0.1439	90	pH 8, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	20.6	0.1961	110	pH 8.5, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	21.8	0.1924	110	pH 9.5, 30 °C	PMID 31873125	Open record: AtSHMT2 (mitochondrial serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT3 (plastid serine hydroxymethyltransferase)	Arabidopsis thaliana	(6S)-5,6,7,8-tetrahydrofolate (tetrahydropteroylmonoglutamate)	15.8	0.2177	70	pH 8.5	PMID 20518745	Open record: AtSHMT3 (plastid serine hydroxymethyltransferase), (6S)-5,6,7,8-tetrahydrofolate (tetrahydropteroylmonoglutamate)
AtSHMT3 (plastid serine hydroxymethyltransferase)	Arabidopsis thaliana	(6S)-tetrahydropteroyldi-gamma-L-glutamate	7.6	0.0133	570	pH 8.5	PMID 20518745	Open record: AtSHMT3 (plastid serine hydroxymethyltransferase), (6S)-tetrahydropteroyldi-gamma-L-glutamate
AtSHMT3 (plastid serine hydroxymethyltransferase)	Arabidopsis thaliana	(6S)-tetrahydropteroylhepta-gamma-L-glutamate	3.8	6.7 × 10⁻⁴	5.67 × 10³	pH 8.5	PMID 20518745	Open record: AtSHMT3 (plastid serine hydroxymethyltransferase), (6S)-tetrahydropteroylhepta-gamma-L-glutamate
AtSHMT3 (plastid serine hydroxymethyltransferase)	Arabidopsis thaliana	(6S)-tetrahydropteroylhexa-gamma-L-glutamate	3.5	6.9 × 10⁻⁴	5.07 × 10³	pH 8.5	PMID 20518745	Open record: AtSHMT3 (plastid serine hydroxymethyltransferase), (6S)-tetrahydropteroylhexa-gamma-L-glutamate
AtSHMT3 (plastid serine hydroxymethyltransferase)	Arabidopsis thaliana	(6S)-tetrahydropteroylocta-gamma-L-glutamate	3.3	5.5 × 10⁻⁴	6 × 10³	pH 8.5	PMID 20518745	Open record: AtSHMT3 (plastid serine hydroxymethyltransferase), (6S)-tetrahydropteroylocta-gamma-L-glutamate
AtSHMT3 (plastid serine hydroxymethyltransferase)	Arabidopsis thaliana	(6S)-tetrahydropteroylpenta-gamma-L-glutamate	3.5	6.4 × 10⁻⁴	5.47 × 10³	pH 8.5	PMID 20518745	Open record: AtSHMT3 (plastid serine hydroxymethyltransferase), (6S)-tetrahydropteroylpenta-gamma-L-glutamate
AtSHMT3 (plastid serine hydroxymethyltransferase)	Arabidopsis thaliana	(6S)-tetrahydropteroyltetra-gamma-L-glutamate	4.3	8.3 × 10⁻⁴	5.18 × 10³	pH 8.5	PMID 20518745	Open record: AtSHMT3 (plastid serine hydroxymethyltransferase), (6S)-tetrahydropteroyltetra-gamma-L-glutamate
AtSHMT3 (plastid serine hydroxymethyltransferase)	Arabidopsis thaliana	(6S)-tetrahydropteroyltri-gamma-L-glutamate	8.7	0.00306	2.84 × 10³	pH 8.5	PMID 20518745	Open record: AtSHMT3 (plastid serine hydroxymethyltransferase), (6S)-tetrahydropteroyltri-gamma-L-glutamate
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	2.56	4.63	pH 6.5, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	0.54	29.97	pH 7, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	0.3	57.89	pH 8, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	0.24	81.53	pH 8.5, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	L-serine	—	0.2	90.75	pH 9.5, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), L-serine
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	11.9	0.00787	1.51 × 10³	pH 6.5, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	16.2	0.01497	1.08 × 10³	pH 7, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	17.4	0.02383	730	pH 8, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	19.6	0.03375	580	pH 8.5, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana)	Arabidopsis thaliana	tetrahydrofolate	18.2	0.04924	370	pH 9.5, 30 °C	PMID 31873125	Open record: AtSHMT4 (cytosolic serine hydroxymethyltransferase, Arabidopsis thaliana), tetrahydrofolate
Essex SHMT8	Glycine max	L-phenylserine	263.3	134.1	1.96		PMID 41914311	Open record: Essex SHMT8, L-phenylserine
Essex SHMT8variant E61K	Glycine max	L-phenylserine	13.65	36.62	0.37		PMID 41914311	Open record: Essex SHMT8 variant E61K, L-phenylserine
Essex SHMT8variant G132D	Glycine max	L-phenylserine	6.32	24.42	0.26		PMID 41914311	Open record: Essex SHMT8 variant G132D, L-phenylserine
Essex SHMT8variant G71D	Glycine max	L-phenylserine	6.39	15.05	0.42		PMID 41914311	Open record: Essex SHMT8 variant G71D, L-phenylserine
Essex SHMT8variant M125I	Glycine max	L-phenylserine	5.31	9.25	0.57		PMID 41914311	Open record: Essex SHMT8 variant M125I, L-phenylserine
Essex SHMT8variant N368T	Glycine max	L-phenylserine	38.5	58.19	0.68		PMID 41914311	Open record: Essex SHMT8 variant N368T, L-phenylserine
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	(6S)-tetrahydrofolate	—	0.005	6 × 10³	pH 6.6, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), (6S)-tetrahydrofolate
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	(6S)-tetrahydrofolate	—	0.011	1909	pH 7.1, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), (6S)-tetrahydrofolate
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	(6S)-tetrahydrofolate	—	0.018	1.1 × 10³	pH 7.5, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), (6S)-tetrahydrofolate
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	(6S)-tetrahydrofolate	—	0.049	404	pH 7.9, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), (6S)-tetrahydrofolate
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	(6S)-tetrahydrofolate	—	0.094	209	pH 8.3, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), (6S)-tetrahydrofolate
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	L-serine	30	0.42	71	pH 6.6, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), L-serine
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	L-serine	21	0.28	75	pH 7.1, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), L-serine
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	L-serine	19.8	0.22	90	pH 7.5, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), L-serine
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	L-serine	19.8	0.18	110	pH 7.9, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), L-serine
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	L-serine	19.6	0.16	123	pH 8.3, 25 °C	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), L-serine
human cytosolic serine hydroxymethyltransferase (hcSHMT)	Homo sapiens	L-serine	4.4	—	—	pH 7	PMID 28760597	Open record: human cytosolic serine hydroxymethyltransferase (hcSHMT), L-serine
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	(6S)-tetrahydrofolate	—	0.051	22	pH 6.6, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), (6S)-tetrahydrofolate
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	(6S)-tetrahydrofolate	—	0.048	29	pH 7, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), (6S)-tetrahydrofolate
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	(6S)-tetrahydrofolate	—	0.054	15	pH 7.3, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), (6S)-tetrahydrofolate
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	(6S)-tetrahydrofolate	—	0.042	14	pH 7.6, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), (6S)-tetrahydrofolate
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	(6S)-tetrahydrofolate	—	0.038	11	pH 8, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), (6S)-tetrahydrofolate
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-serine	1.1	0.3	3.7	pH 6.6, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), L-serine
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-serine	1.41	0.23	6.1	pH 7, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), L-serine
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-serine	0.81	0.2	4.1	pH 7.3, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), L-serine
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-serine	0.57	0.11	5.2	pH 7.6, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), L-serine
Plasmodium vivax serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-serine	0.41	0.12	3.4	pH 8, 25 °C	PMID 28760597	Open record: Plasmodium vivax serine hydroxymethyltransferase (PvSHMT), L-serine
serine hydroxymethyltransferase	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.007	—	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase, (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferase	Escherichia coli	L-allo-threonine	0.5	1.5	0.33 calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase, L-allo-threonine
serine hydroxymethyltransferase	Escherichia coli	L-serine	11.5	0.14	82 calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase, L-serine
serine hydroxymethyltransferasevariant R235K	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.14	—	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant R235K, (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferasevariant R235K	Escherichia coli	L-allo-threonine	0.0025	96	2.6 × 10⁻⁵ calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant R235K, L-allo-threonine
serine hydroxymethyltransferasevariant R235K	Escherichia coli	L-serine	0.8	218	0.0037 calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant R235K, L-serine
serine hydroxymethyltransferasevariant R235L	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.08	—	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant R235L, (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferasevariant R235L	Escherichia coli	L-serine	0.2	64	0.0031 calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant R235L, L-serine
serine hydroxymethyltransferasevariant R235Q	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.12	—	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant R235Q, (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferasevariant R235Q	Escherichia coli	L-allo-threonine	0.0033	95	3.5 × 10⁻⁵ calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant R235Q, L-allo-threonine
serine hydroxymethyltransferasevariant R235Q	Escherichia coli	L-serine	0.33	127	0.0026 calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant R235Q, L-serine
serine hydroxymethyltransferasevariant Y55F	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.11	—	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant Y55F, (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferasevariant Y55F	Escherichia coli	L-allo-threonine	0.02	57	3.5 × 10⁻⁴ calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant Y55F, L-allo-threonine
serine hydroxymethyltransferasevariant Y55F	Escherichia coli	L-serine	3	7	0.43 calc.	pH 7.2, 30 °C	PMID 19883126	Open record: serine hydroxymethyltransferase variant Y55F, L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	5,10-methylene-tetrahydrofolate0 M NaCl	0.22	0.2	1.1 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	5,10-methylene-tetrahydrofolate0.125 M NaCl	0.193	0.22	0.88 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	5,10-methylene-tetrahydrofolate0.250 M NaCl	0.168	0.22	0.77 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	5,10-methylene-tetrahydrofolate0.375 M NaCl	0.17	0.29	0.59 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	5,10-methylene-tetrahydrofolate0.500 M NaCl	0.158	0.42	0.38 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	glycine0 M NaCl	—	0.25	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), glycine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	glycine0.125 M NaCl	—	0.49	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), glycine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	glycine0.250 M NaCl	—	0.91	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), glycine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	glycine0.375 M NaCl	—	1.18	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), glycine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	glycine0.500 M NaCl	—	1.22	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), glycine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-allo-threonine	1.88	1.4	1.3 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-allo-threonine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-erythro-phenylserine	95	6.9	14 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-erythro-phenylserine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine	—	5.07	—	pH 6	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine	—	1.07	—	pH 6.5	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine	—	0.39	—	pH 7	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine	—	0.31	—	pH 8	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine	—	0.3	—	pH 9	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine0 M NaCl	—	0.51	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine0.125 M NaCl	—	1.13	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine0.250 M NaCl	—	1.52	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine0.375 M NaCl	—	1.93	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine0.500 M NaCl	—	2.22	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-serine0.750 M NaCl	—	5.47	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-serine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-threo-phenylserine	6.77	32	0.21 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-threo-phenylserine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	L-threonine	0.057	5.2	0.011 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), L-threonine
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate	78.7	0.08	980 calc.	pH 6	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate	81.23	0.1	810 calc.	pH 6.5	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate	68	0.13	520 calc.	pH 7	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate	48.97	0.18	270 calc.	pH 8	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate	40.6	0.13	310 calc.	pH 9	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate0 M NaCl	74.45	0.08	930 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate0.125 M NaCl	65.37	0.1	650 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate0.250 M NaCl	56.63	0.11	510 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate0.375 M NaCl	46.57	0.13	360 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate0.500 M NaCl	39.52	0.25	160 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (AhSHMT)	Aphanothece halophytica	tetrahydrofolate0.750 M NaCl	21.52	0.37	58 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate
serine hydroxymethyltransferase (bmSHMT)	Bombyx mori	L-serine	—	1.8	0.0022	pH 3, 30 °C	PMID 31298425	Open record: serine hydroxymethyltransferase (bmSHMT), L-serine
serine hydroxymethyltransferase (bmSHMT)	Bombyx mori	tetrahydrofolate	—	0.055	0.081	pH 3, 30 °C	PMID 31298425	Open record: serine hydroxymethyltransferase (bmSHMT), tetrahydrofolate
serine hydroxymethyltransferase (bmSHMT)variant H119A	Bombyx mori	L-serine	—	4.2	6.5 × 10⁻⁴	pH 3, 30 °C	PMID 31298425	Open record: serine hydroxymethyltransferase (bmSHMT) variant H119A, L-serine
serine hydroxymethyltransferase (bmSHMT)variant H119A	Bombyx mori	tetrahydrofolate	—	0.031	0.074	pH 3, 30 °C	PMID 31298425	Open record: serine hydroxymethyltransferase (bmSHMT) variant H119A, tetrahydrofolate
serine hydroxymethyltransferase (bmSHMT)variant H132A	Bombyx mori	tetrahydrofolate	—	0.14	0.011	pH 3, 30 °C	PMID 31298425	Open record: serine hydroxymethyltransferase (bmSHMT) variant H132A, tetrahydrofolate
serine hydroxymethyltransferase (bmSHMT)variant H135A	Bombyx mori	L-serine	—	3.3	7.1 × 10⁻⁴	pH 3, 30 °C	PMID 31298425	Open record: serine hydroxymethyltransferase (bmSHMT) variant H135A, L-serine
serine hydroxymethyltransferase (bmSHMT)variant H135A	Bombyx mori	tetrahydrofolate	—	0.03	0.047	pH 3, 30 °C	PMID 31298425	Open record: serine hydroxymethyltransferase (bmSHMT) variant H135A, tetrahydrofolate
serine hydroxymethyltransferase (bsSHMT)	Bacillus stearothermophilus	L-allothreoninedimeric form (90 kDa, Superose-12 pool)	0.9	0.4	2.3 calc.		PMID 12089472	Open record: serine hydroxymethyltransferase (bsSHMT), L-allothreonine
serine hydroxymethyltransferase (bsSHMT)	Bacillus stearothermophilus	L-allothreonine	0.7	0.7	1.0 calc.		PMID 12089472	Open record: serine hydroxymethyltransferase (bsSHMT), L-allothreonine
serine hydroxymethyltransferase (bsSHMT)	Bacillus stearothermophilus	L-serinedimeric form (90 kDa, Superose-12 pool)	5	0.9	5.6 calc.	pH 7.4, 37 °C	PMID 12089472	Open record: serine hydroxymethyltransferase (bsSHMT), L-serine
serine hydroxymethyltransferase (bsSHMT)	Bacillus stearothermophilus	L-serine	3.6	0.7	5.1 calc.	pH 7.4, 37 °C	PMID 12089472	Open record: serine hydroxymethyltransferase (bsSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.00703	—	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT), (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	5,10-methylene-tetrahydrofolate0 M NaCl	0.195	0.18	1.1 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	5,10-methylene-tetrahydrofolate0.125 M NaCl	0.202	0.23	0.88 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	5,10-methylene-tetrahydrofolate0.250 M NaCl	0.213	0.27	0.79 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	5,10-methylene-tetrahydrofolate0.375 M NaCl	0.225	0.35	0.64 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	5,10-methylene-tetrahydrofolate0.500 M NaCl	0.23	0.43	0.53 calc.	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	glycine0 M NaCl	—	0.52	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), glycine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	glycine0.125 M NaCl	—	0.85	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), glycine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	glycine0.250 M NaCl	—	1	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), glycine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	glycine0.375 M NaCl	—	2.29	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), glycine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	glycine0.500 M NaCl	—	5.87	—	pH 8.8, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), glycine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-allo-threonine	0.43	2	0.22 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-allo-threonine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-erythro-phenylserine	52.37	54	0.97 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-erythro-phenylserine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine	5	0.3	17 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine	11.44	0.14	82 calc.	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine	—	0.57	—	pH 6.5	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine	—	0.26	—	pH 7	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine	—	0.15	—	pH 8	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine	—	0.13	—	pH 9	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine0 M NaCl	—	0.39	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine0.125 M NaCl	—	2.06	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine0.250 M NaCl	—	4.14	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine0.375 M NaCl	—	8.97	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine0.500 M NaCl	—	11	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-serine0.750 M NaCl	—	20.66	—	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-threo-3-phenylserine	3.368	36.3	0.0928 calc.	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT), L-threo-3-phenylserine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-threo-phenylserine	2.43	33	0.074 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-threo-phenylserine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	L-threonine	0.05	42	0.0012 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), L-threonine
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate	58.8	0.1	590 calc.	pH 6.5	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate	40.13	0.12	330 calc.	pH 7	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate	26.8	0.12	220 calc.	pH 8	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate	20.7	0.13	160 calc.	pH 9	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate0 M NaCl	42.7	0.04	1.1 × 10³ calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate0.125 M NaCl	28.5	0.06	480 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate0.250 M NaCl	27.3	0.09	300 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate0.375 M NaCl	21.8	0.11	200 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate0.500 M NaCl	15.8	0.14	110 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)	Escherichia coli	tetrahydrofolate0.750 M NaCl	8.1	0.22	37 calc.	pH 7.2, 30 °C	PMID 32417544	Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate
serine hydroxymethyltransferase (eSHMT)variant L276A	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.00435	—	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L276A, (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferase (eSHMT)variant L276A	Escherichia coli	L-serine	6.675	0.2	33 calc.	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L276A, L-serine
serine hydroxymethyltransferase (eSHMT)variant L276A	Escherichia coli	L-threo-3-phenylserine	2.205	42.6	0.0518 calc.	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L276A, L-threo-3-phenylserine
serine hydroxymethyltransferase (eSHMT)variant L85A	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.00716	—	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L85A, (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferase (eSHMT)variant L85A	Escherichia coli	L-serine	10.79	0.15	72 calc.	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L85A, L-serine
serine hydroxymethyltransferase (eSHMT)variant L85A	Escherichia coli	L-threo-3-phenylserine	4.297	37.4	0.115 calc.	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L85A, L-threo-3-phenylserine
serine hydroxymethyltransferase (eSHMT)variant L85A/L276A	Escherichia coli	(6S)-tetrahydropteroylglutamate	—	0.0112	—	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L85A/L276A, (6S)-tetrahydropteroylglutamate
serine hydroxymethyltransferase (eSHMT)variant L85A/L276A	Escherichia coli	L-serine	6.67	0.2	33 calc.	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L85A/L276A, L-serine
serine hydroxymethyltransferase (eSHMT)variant L85A/L276A	Escherichia coli	L-threo-3-phenylserine	2.898	42.1	0.0688 calc.	pH 7.2, 20 °C	PMID 19019081	Open record: serine hydroxymethyltransferase (eSHMT) variant L85A/L276A, L-threo-3-phenylserine
serine hydroxymethyltransferase (eSHMT)variant W16F/W183F/P264A (P264A, in W385 eSHMT)	Escherichia coli	L-serine	1.63	0.33	4.9 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT) variant W16F/W183F/P264A (P264A, in W385 eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)variant W16F/W183F/P264G (P264G, in W385 eSHMT)	Escherichia coli	L-serine	0.12	1.33	0.088 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT) variant W16F/W183F/P264G (P264G, in W385 eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)variant W16F/W385F/P214A (P214A, in W183 eSHMT)	Escherichia coli	L-serine	3.3	0.14	24 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT) variant W16F/W385F/P214A (P214A, in W183 eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)variant W16F/W385F/P214G (P214G, in W183 eSHMT)	Escherichia coli	L-serine	3.3	0.24	14 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT) variant W16F/W385F/P214G (P214G, in W183 eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)variant W16F/W385F/P216A (P216A, in W183 eSHMT)	Escherichia coli	L-serine	3.3	0.25	13 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT) variant W16F/W385F/P216A (P216A, in W183 eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)variant W16F/W385F/P216G (P216G, in W183 eSHMT)	Escherichia coli	L-serine	0.6	0.58	1.0 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT) variant W16F/W385F/P216G (P216G, in W183 eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)variant W16F/W385F/P218A (P218A, in W183 eSHMT)	Escherichia coli	L-serine	4.5	0.13	35 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT) variant W16F/W385F/P218A (P218A, in W183 eSHMT), L-serine
serine hydroxymethyltransferase (eSHMT)variant W16F/W385F/P218G (P218G, in W183 eSHMT)	Escherichia coli	L-serine	3.3	0.13	26 calc.		PMID 12773539	Open record: serine hydroxymethyltransferase (eSHMT) variant W16F/W385F/P218G (P218G, in W183 eSHMT), L-serine
serine hydroxymethyltransferase (PfSHMT)	Plasmodium falciparum	D-serine	0.246	71	0.00346	pH 8, 25 °C	PMID 19591881	Open record: serine hydroxymethyltransferase (PfSHMT), D-serine
serine hydroxymethyltransferase (PfSHMT)	Plasmodium falciparum	L-serineTable 1	0.74	0.371	2.0 calc.	pH 7.45, 25 °C	PMID 19591881	Open record: serine hydroxymethyltransferase (PfSHMT), L-serine
serine hydroxymethyltransferase (PfSHMT)	Plasmodium falciparum	L-serinein the text	—	—	1.99	pH 7.45, 25 °C	PMID 19591881	Open record: serine hydroxymethyltransferase (PfSHMT), L-serine
serine hydroxymethyltransferase (PfSHMT)	Plasmodium falciparum	L-serine	0.43	0.251	1.7 calc.	25 °C	PMID 19591881	Open record: serine hydroxymethyltransferase (PfSHMT), L-serine
serine hydroxymethyltransferase (PfSHMT)	Plasmodium falciparum	tetrahydrofolateTable 1	—	0.193	—	pH 7.45, 25 °C	PMID 19591881	Open record: serine hydroxymethyltransferase (PfSHMT), tetrahydrofolate
serine hydroxymethyltransferase (PfSHMT)	Plasmodium falciparum	tetrahydrofolatein the text	—	—	3.83	pH 7.45, 25 °C	PMID 19591881	Open record: serine hydroxymethyltransferase (PfSHMT), tetrahydrofolate
serine hydroxymethyltransferase (PfSHMT)	Plasmodium falciparum	tetrahydrofolate	—	0.253	—	25 °C	PMID 19591881	Open record: serine hydroxymethyltransferase (PfSHMT), tetrahydrofolate
serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	(6R,S)-tetrahydrofolate	—	0.14	—	pH 7, 25 °C	PMID 19549189	Open record: serine hydroxymethyltransferase (PvSHMT), (6R,S)-tetrahydrofolate
serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	D,L-threo-β-phenylserine	0.12	8.6	0.014 calc.	25 °C	PMID 19549189	Open record: serine hydroxymethyltransferase (PvSHMT), D,L-threo-β-phenylserine
serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	D-serine	0.26	47	0.0055 calc.	pH 7, 25 °C	PMID 19549189	Open record: serine hydroxymethyltransferase (PvSHMT), D-serine
serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-allo-threonine	0.044	0.32	0.14 calc.	25 °C	PMID 19549189	Open record: serine hydroxymethyltransferase (PvSHMT), L-allo-threonine
serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-serine	0.98	0.18	5.4 calc.	pH 7, 25 °C	PMID 19549189	Open record: serine hydroxymethyltransferase (PvSHMT), L-serine
serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-serine	0.8	—	—	25 °C	PMID 19549189	Open record: serine hydroxymethyltransferase (PvSHMT), L-serine
serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-serine	1.3	—	—	30 °C	PMID 19549189	Open record: serine hydroxymethyltransferase (PvSHMT), L-serine
serine hydroxymethyltransferase (PvSHMT)	Plasmodium vivax	L-threonine	0.015	7	0.0021 calc.	25 °C	PMID 19549189	Open record: serine hydroxymethyltransferase (PvSHMT), L-threonine
serine hydroxymethyltransferase (scSHMT, sheep liver cytosolic)	Ovis aries (sheep)	L-allothreonine	3.7	0.7	5.3 calc.		PMID 12089472	Open record: serine hydroxymethyltransferase (scSHMT, sheep liver cytosolic), L-allothreonine
serine hydroxymethyltransferase (scSHMT, sheep liver cytosolic)	Ovis aries (sheep)	L-serine	4.3	1	4.3 calc.	pH 7.4, 37 °C	PMID 12089472	Open record: serine hydroxymethyltransferase (scSHMT, sheep liver cytosolic), L-serine
serine hydroxymethyltransferase, human cytosolic (hcSHMT)	Homo sapiens	(6S)-tetrahydrofolatecosubstrate L-serine	—	0.0052	—	pH 7, 25 °C	PMID 24698160	Open record: serine hydroxymethyltransferase, human cytosolic (hcSHMT), (6S)-tetrahydrofolate
serine hydroxymethyltransferase, human cytosolic (hcSHMT)	Homo sapiens	(6S)-tetrahydrofolate	—	0.007	—	25 °C	PMID 24698160	Open record: serine hydroxymethyltransferase, human cytosolic (hcSHMT), (6S)-tetrahydrofolate
serine hydroxymethyltransferase, human cytosolic (hcSHMT)	Homo sapiens	(6S)-tetrahydrofolateD-serine (10-1280 mM) and (6S)-THF (5-40 µM)	—	0.021	—	pH 7, 25 °C	PMID 24698160	Open record: serine hydroxymethyltransferase, human cytosolic (hcSHMT), (6S)-tetrahydrofolate
serine hydroxymethyltransferase, human cytosolic (hcSHMT)	Homo sapiens	D-serine	9.46	55	0.172	pH 7, 25 °C	PMID 24698160	Open record: serine hydroxymethyltransferase, human cytosolic (hcSHMT), D-serine
serine hydroxymethyltransferase, human cytosolic (hcSHMT)	Homo sapiens	L-serine	28	0.18	156	pH 7, 25 °C	PMID 24698160	Open record: serine hydroxymethyltransferase, human cytosolic (hcSHMT), L-serine
serine hydroxymethyltransferase, human cytosolic (hcSHMT)	Homo sapiens	L-serine	15	0.17	88	25 °C	PMID 24698160	Open record: serine hydroxymethyltransferase, human cytosolic (hcSHMT), L-serine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	5,10-methylenetetrahydrofolate	—	0.291	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), 5,10-methylenetetrahydrofolate
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	5,10-methylenetetrahydrofolate	—	0.159	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), 5,10-methylenetetrahydrofolate
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	5,10-methylenetetrahydrofolate	—	—	0.65	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), 5,10-methylenetetrahydrofolate
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	5,10-methylenetetrahydrofolate	—	—	1.18	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), 5,10-methylenetetrahydrofolate
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	glycine	0.188	—	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), glycine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	glycine	—	0.556	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), glycine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	glycine	—	0.514	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), glycine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	glycine	—	—	0.33	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), glycine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	glycine	—	—	0.37	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), glycine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	L-serine	6.23	—	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), L-serine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	4.8	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), L-serine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	0.131	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), L-serine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	—	1.3	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), L-serine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	—	47.5	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), L-serine
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	0.033	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), tetrahydrofolate
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	0.007	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), tetrahydrofolate
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	—	188.8	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), tetrahydrofolate
SHMT1 (cytosolic serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	—	890.5	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT1 (cytosolic serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	5,10-methylenetetrahydrofolatelike another record	—	0.98	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), 5,10-methylenetetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	5,10-methylenetetrahydrofolatelike another record	—	0.98	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), 5,10-methylenetetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	5,10-methylenetetrahydrofolatelike another record	—	—	0.52	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), 5,10-methylenetetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	5,10-methylenetetrahydrofolatelike another record	—	—	0.52	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), 5,10-methylenetetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	glycine	0.5012	—	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), glycine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	glycine	—	0.66	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), glycine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	glycine	—	0.47	—	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), glycine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	glycine	—	—	0.77	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), glycine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	glycine	—	—	1.07	pH 8.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), glycine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	0.278	—	pH 7.2, 20 °C	PMID 25619277	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	14.17	—	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	2.2	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	0.396	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	—	6.5	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	—	35.8	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	13.55	—	—	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	0.45	—	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	0.158	—	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	—	30.2	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	L-serine	—	—	85.8	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), L-serine
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	0.023	—	pH 7.2, 20 °C	PMID 25619277	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	0.223	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	0.053	—	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	—	63.5	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	—	267.3	pH 7.2, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	0.032	—	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	0.02	—	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	—	423	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate
SHMT2 (mitochondrial serine hydroxymethyltransferase)	Homo sapiens	tetrahydrofolate	—	—	677.5	pH 7.8, 30 °C	PMID 30500180	Open record: SHMT2 (mitochondrial serine hydroxymethyltransferase), tetrahydrofolate