Values CatLog read from this paper
Role of a conserved active site cation-pi interaction in Escherichia coli serine hydroxymethyltransferase
PMID 19883126doi:10.1021/bi901568bOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (23 values) from this paper: serine hydroxymethyltransferase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| serine hydroxymethyltransferase | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.007 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase, (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferase | Escherichia coli | L-allo-threonine | 0.5 | 1.5 | 0.33 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase, L-allo-threonine |
| serine hydroxymethyltransferase | Escherichia coli | L-serine | 11.5 | 0.14 | 82 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase, L-serine |
| serine hydroxymethyltransferasevariant R235K | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.14 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant R235K, (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferasevariant R235K | Escherichia coli | L-allo-threonine | 0.0025 | 96 | 2.6 × 10⁻⁵ calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant R235K, L-allo-threonine |
| serine hydroxymethyltransferasevariant R235K | Escherichia coli | L-serine | 0.8 | 218 | 0.0037 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant R235K, L-serine |
| serine hydroxymethyltransferasevariant R235L | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.08 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant R235L, (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferasevariant R235L | Escherichia coli | L-serine | 0.2 | 64 | 0.0031 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant R235L, L-serine |
| serine hydroxymethyltransferasevariant R235Q | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.12 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant R235Q, (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferasevariant R235Q | Escherichia coli | L-allo-threonine | 0.0033 | 95 | 3.5 × 10⁻⁵ calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant R235Q, L-allo-threonine |
| serine hydroxymethyltransferasevariant R235Q | Escherichia coli | L-serine | 0.33 | 127 | 0.0026 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant R235Q, L-serine |
| serine hydroxymethyltransferasevariant Y55F | Escherichia coli | (6S)-tetrahydropteroylglutamate | — | 0.11 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant Y55F, (6S)-tetrahydropteroylglutamate |
| serine hydroxymethyltransferasevariant Y55F | Escherichia coli | L-allo-threonine | 0.02 | 57 | 3.5 × 10⁻⁴ calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant Y55F, L-allo-threonine |
| serine hydroxymethyltransferasevariant Y55F | Escherichia coli | L-serine | 3 | 7 | 0.43 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase variant Y55F, L-serine |