Values CatLog read from this paper
Structural and kinetic properties of serine hydroxymethyltransferase from the halophytic cyanobacterium Aphanothece halophytica provide a rationale for salt tolerance
PMID 32417544doi:10.1016/j.ijbiomac.2020.05.081Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 70 records (109 values) from this paper: serine hydroxymethyltransferase (AhSHMT), serine hydroxymethyltransferase (eSHMT) from Aphanothece halophytica, Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 39 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | 5,10-methylene-tetrahydrofolate0 M NaCl | 0.22 | 0.2 | 1.1 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | 5,10-methylene-tetrahydrofolate0.125 M NaCl | 0.193 | 0.22 | 0.88 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | 5,10-methylene-tetrahydrofolate0.250 M NaCl | 0.168 | 0.22 | 0.77 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | 5,10-methylene-tetrahydrofolate0.375 M NaCl | 0.17 | 0.29 | 0.59 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | 5,10-methylene-tetrahydrofolate0.500 M NaCl | 0.158 | 0.42 | 0.38 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | glycine0 M NaCl | — | 0.25 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), glycine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | glycine0.125 M NaCl | — | 0.49 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), glycine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | glycine0.250 M NaCl | — | 0.91 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), glycine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | glycine0.375 M NaCl | — | 1.18 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), glycine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | glycine0.500 M NaCl | — | 1.22 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), glycine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-allo-threonine | 1.88 | 1.4 | 1.3 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-allo-threonine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-erythro-phenylserine | 95 | 6.9 | 14 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-erythro-phenylserine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine | — | 5.07 | — | pH 6 | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine | — | 1.07 | — | pH 6.5 | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine | — | 0.39 | — | pH 7 | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine | — | 0.31 | — | pH 8 | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine | — | 0.3 | — | pH 9 | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine0 M NaCl | — | 0.51 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine0.125 M NaCl | — | 1.13 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine0.250 M NaCl | — | 1.52 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine0.375 M NaCl | — | 1.93 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine0.500 M NaCl | — | 2.22 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-serine0.750 M NaCl | — | 5.47 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-serine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-threo-phenylserine | 6.77 | 32 | 0.21 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-threo-phenylserine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | L-threonine | 0.057 | 5.2 | 0.011 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), L-threonine |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate | 78.7 | 0.08 | 980 calc. | pH 6 | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate | 81.23 | 0.1 | 810 calc. | pH 6.5 | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate | 68 | 0.13 | 520 calc. | pH 7 | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate | 48.97 | 0.18 | 270 calc. | pH 8 | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate | 40.6 | 0.13 | 310 calc. | pH 9 | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate0 M NaCl | 74.45 | 0.08 | 930 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate0.125 M NaCl | 65.37 | 0.1 | 650 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate0.250 M NaCl | 56.63 | 0.11 | 510 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate0.375 M NaCl | 46.57 | 0.13 | 360 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate0.500 M NaCl | 39.52 | 0.25 | 160 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (AhSHMT) | Aphanothece halophytica | tetrahydrofolate0.750 M NaCl | 21.52 | 0.37 | 58 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (AhSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | 5,10-methylene-tetrahydrofolate0 M NaCl | 0.195 | 0.18 | 1.1 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | 5,10-methylene-tetrahydrofolate0.125 M NaCl | 0.202 | 0.23 | 0.88 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | 5,10-methylene-tetrahydrofolate0.250 M NaCl | 0.213 | 0.27 | 0.79 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | 5,10-methylene-tetrahydrofolate0.375 M NaCl | 0.225 | 0.35 | 0.64 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | 5,10-methylene-tetrahydrofolate0.500 M NaCl | 0.23 | 0.43 | 0.53 calc. | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), 5,10-methylene-tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | glycine0 M NaCl | — | 0.52 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), glycine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | glycine0.125 M NaCl | — | 0.85 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), glycine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | glycine0.250 M NaCl | — | 1 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), glycine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | glycine0.375 M NaCl | — | 2.29 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), glycine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | glycine0.500 M NaCl | — | 5.87 | — | pH 8.8, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), glycine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-allo-threonine | 0.43 | 2 | 0.22 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-allo-threonine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-erythro-phenylserine | 52.37 | 54 | 0.97 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-erythro-phenylserine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine | — | 0.57 | — | pH 6.5 | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine | — | 0.26 | — | pH 7 | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine | — | 0.15 | — | pH 8 | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine | — | 0.13 | — | pH 9 | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine0 M NaCl | — | 0.39 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine0.125 M NaCl | — | 2.06 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine0.250 M NaCl | — | 4.14 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine0.375 M NaCl | — | 8.97 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine0.500 M NaCl | — | 11 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-serine0.750 M NaCl | — | 20.66 | — | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-serine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-threo-phenylserine | 2.43 | 33 | 0.074 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-threo-phenylserine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | L-threonine | 0.05 | 42 | 0.0012 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), L-threonine |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate | 58.8 | 0.1 | 590 calc. | pH 6.5 | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate | 40.13 | 0.12 | 330 calc. | pH 7 | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate | 26.8 | 0.12 | 220 calc. | pH 8 | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate | 20.7 | 0.13 | 160 calc. | pH 9 | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate0 M NaCl | 42.7 | 0.04 | 1.1 × 10³ calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate0.125 M NaCl | 28.5 | 0.06 | 480 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate0.250 M NaCl | 27.3 | 0.09 | 300 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate0.375 M NaCl | 21.8 | 0.11 | 200 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate0.500 M NaCl | 15.8 | 0.14 | 110 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |
| serine hydroxymethyltransferase (eSHMT) | Escherichia coli | tetrahydrofolate0.750 M NaCl | 8.1 | 0.22 | 37 calc. | pH 7.2, 30 °C | Open record: serine hydroxymethyltransferase (eSHMT), tetrahydrofolate |