Values CatLog read from this paper
Structure and function of 2,3-dimethylmalate lyase, a PEP mutase/isocitrate lyase superfamily member
PMID 19133276doi:10.1016/j.jmb.2008.12.037Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (54 values) from this paper: (2R,3S)-dimethylmalate lyase (DMML) from Aspergillus niger.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R)-2-ethylmalate | 4.4 | 12 | 0.1 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R)-2-ethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R)-2-methylmalate | 2.64 | 2.2 | 1 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R)-2-methylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R,3S)- and (2S,3R)-2-methylisocitrate (threo isomers) | 0.13 | 12 | 0.01 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R,3S)- and (2S,3R)-2-methylisocitrate (threo isomers) |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 19.2 | 0.22 | 90 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R,3S)-2-ethyl-3-methylmalate | 63 | 1.17 | 50 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R,3S)-2-ethyl-3-methylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R,3S)-2-propyl-3-methylmalate | 0.79 | 2.7 | 0.3 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R,3S)-2-propyl-3-methylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | oxaloacetate | 0.505 | 0.234 | 2 | pH 7.6, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML), oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | oxaloacetate | 0.48 | 0.22 | 2 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML), oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML)variant C124A | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 1.78 × 10⁻⁴ | 0.036 | 0.005 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant C124A, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant C124A | Aspergillus niger | oxaloacetate | 0.125 | 0.142 | 0.9 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant C124A, oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML)variant C124S | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 1.21 × 10⁻⁴ | 3.6 | 3 × 10⁻⁵ | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant C124S, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant C124S | Aspergillus niger | oxaloacetate | 0.081 | 0.027 | 3 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant C124S, oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML)variant D59A | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 4.27 × 10⁻⁴ | 7.4 | 6 × 10⁻⁵ | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant D59A, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant D59S | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 1.4 × 10⁻⁴ | 6 | 2 × 10⁻⁵ | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant D59S, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant P240S | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 11.7 | 5.9 | 2 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant P240S, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant P240S | Aspergillus niger | oxaloacetate | 0.357 | 1.11 | 0.3 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant P240S, oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML)variant P240T | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 2.8 | 4.1 | 0.7 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant P240T, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant P240T | Aspergillus niger | oxaloacetate | 0.082 | 1.22 | 0.07 | pH 7.5, 25 °C | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant P240T, oxaloacetate |