EC 4.1.3.1: isocitrate lyase
LyasesOpen in Browse
CatLog read 31 records for EC 4.1.3.1 from 4 papers, for 3 organisms. Names in these papers: (2R,3S)-dimethylmalate lyase (DMML), isocitrate lyase (ICL-Pa), isocitrate lyase (MtbICL), isocitrate lyase 1 (MtICL1).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 105 records for EC 4.1.3.1 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.3.1 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Structure and function of 2,3-dimethylmalate lyase, a PEP mutase/isocitrate lyase superfamily member | Aspergillus niger | 18 |
| Residues Asn214, Gln211, Glu219 and Gln221 contained in the subfamily 3 catalytic signature of the isocitrate lyase from Pseudomonas aeruginosa are involved in its catalytic and thermal properties | Pseudomonas aeruginosa | 5 |
| Insight into the structural flexibility and function of Mycobacterium tuberculosis isocitrate lyase | Mycobacterium tuberculosis | 4 |
| Substrate Specificity and Kinetic Mechanism of the NAD+-Dependent Deacylase from Mycobacterium tuberculosis, Rv1151c (Mt-Sirt) | Mycobacterium tuberculosis | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R)-2-ethylmalate | 4.4 | 12 | 0.1 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R)-2-ethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R)-2-methylmalate | 2.64 | 2.2 | 1 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R)-2-methylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R,3S)- and (2S,3R)-2-methylisocitrate (threo isomers) | 0.13 | 12 | 0.01 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R,3S)- and (2S,3R)-2-methylisocitrate (threo isomers) |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 19.2 | 0.22 | 90 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R,3S)-2-ethyl-3-methylmalate | 63 | 1.17 | 50 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R,3S)-2-ethyl-3-methylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | (2R,3S)-2-propyl-3-methylmalate | 0.79 | 2.7 | 0.3 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML), (2R,3S)-2-propyl-3-methylmalate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | oxaloacetate | 0.505 | 0.234 | 2 | pH 7.6, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML), oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML) | Aspergillus niger | oxaloacetate | 0.48 | 0.22 | 2 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML), oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML)variant C124A | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 1.78 × 10⁻⁴ | 0.036 | 0.005 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant C124A, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant C124A | Aspergillus niger | oxaloacetate | 0.125 | 0.142 | 0.9 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant C124A, oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML)variant C124S | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 1.21 × 10⁻⁴ | 3.6 | 3 × 10⁻⁵ | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant C124S, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant C124S | Aspergillus niger | oxaloacetate | 0.081 | 0.027 | 3 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant C124S, oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML)variant D59A | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 4.27 × 10⁻⁴ | 7.4 | 6 × 10⁻⁵ | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant D59A, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant D59S | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 1.4 × 10⁻⁴ | 6 | 2 × 10⁻⁵ | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant D59S, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant P240S | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 11.7 | 5.9 | 2 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant P240S, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant P240S | Aspergillus niger | oxaloacetate | 0.357 | 1.11 | 0.3 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant P240S, oxaloacetate |
| (2R,3S)-dimethylmalate lyase (DMML)variant P240T | Aspergillus niger | (2R,3S)-2,3-dimethylmalate | 2.8 | 4.1 | 0.7 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant P240T, (2R,3S)-2,3-dimethylmalate |
| (2R,3S)-dimethylmalate lyase (DMML)variant P240T | Aspergillus niger | oxaloacetate | 0.082 | 1.22 | 0.07 | pH 7.5, 25 °C | PMID 19133276 | Open record: (2R,3S)-dimethylmalate lyase (DMML) variant P240T, oxaloacetate |
| isocitrate lyase (ICL-Pa) | Pseudomonas aeruginosa | DL-isocitric acid | 9.81 | 0.44 | 22.3 | pH 7.8, 30 °C | PMID 23338961 | Open record: isocitrate lyase (ICL-Pa), DL-isocitric acid |
| isocitrate lyase (ICL-Pa)variant E219A | Pseudomonas aeruginosa | DL-isocitric acid | 4.63 | 0.94 | 4.9 | pH 7.8, 30 °C | PMID 23338961 | Open record: isocitrate lyase (ICL-Pa) variant E219A, DL-isocitric acid |
| isocitrate lyase (ICL-Pa)variant N214D | Pseudomonas aeruginosa | DL-isocitric acid | 0.56 | 0.25 | 2.8 | pH 7.8, 30 °C | PMID 23338961 | Open record: isocitrate lyase (ICL-Pa) variant N214D, DL-isocitric acid |
| isocitrate lyase (ICL-Pa)variant Q211H | Pseudomonas aeruginosa | DL-isocitric acid | 3.46 | 0.29 | 11.9 | pH 7.8, 30 °C | PMID 23338961 | Open record: isocitrate lyase (ICL-Pa) variant Q211H, DL-isocitric acid |
| isocitrate lyase (ICL-Pa)variant Q221K | Pseudomonas aeruginosa | DL-isocitric acid | 3.25 | 0.47 | 6.9 | pH 7.8, 30 °C | PMID 23338961 | Open record: isocitrate lyase (ICL-Pa) variant Q221K, DL-isocitric acid |
| isocitrate lyase (MtbICL) | Mycobacterium tuberculosis | threo-DL-isocitrate | 9.78 | 1.142 | 8.56 calc. | pH 7.5, 30 °C | PMID 25576828 | Open record: isocitrate lyase (MtbICL), threo-DL-isocitrate |
| isocitrate lyase (MtbICL)variant E423D | Mycobacterium tuberculosis | threo-DL-isocitrate | 8.34 | 1.423 | 5.86 calc. | pH 7.5, 30 °C | PMID 25576828 | Open record: isocitrate lyase (MtbICL) variant E423D, threo-DL-isocitrate |
| isocitrate lyase (MtbICL)variant E423D/E424D | Mycobacterium tuberculosis | threo-DL-isocitrate | 7.53 | 2.031 | 3.71 calc. | pH 7.5, 30 °C | PMID 25576828 | Open record: isocitrate lyase (MtbICL) variant E423D/E424D, threo-DL-isocitrate |
| isocitrate lyase (MtbICL)variant E424D | Mycobacterium tuberculosis | threo-DL-isocitrate | 8.46 | 1.861 | 4.55 calc. | pH 7.5, 30 °C | PMID 25576828 | Open record: isocitrate lyase (MtbICL) variant E424D, threo-DL-isocitrate |
| isocitrate lyase 1 (MtICL1) | Mycobacterium tuberculosis | D-isocitrate | 22 | 0.051 | 430 | pH 7.5, 37 °C | PMID 42441379 | Open record: isocitrate lyase 1 (MtICL1), D-isocitrate |
| isocitrate lyase 1 (MtICL1)variant K189E | Mycobacterium tuberculosis | D-isocitrate | 0.002 | 0.022 | 0.095 | pH 7.5, 37 °C | PMID 42441379 | Open record: isocitrate lyase 1 (MtICL1) variant K189E, D-isocitrate |
| isocitrate lyase 1 (MtICL1)variant K322E | Mycobacterium tuberculosis | D-isocitrate | 38 | 0.097 | 390 | pH 7.5, 37 °C | PMID 42441379 | Open record: isocitrate lyase 1 (MtICL1) variant K322E, D-isocitrate |
| isocitrate lyase 1 (MtICL1)variant K334E | Mycobacterium tuberculosis | D-isocitrate | 21 | 0.057 | 370 | pH 7.5, 37 °C | PMID 42441379 | Open record: isocitrate lyase 1 (MtICL1) variant K334E, D-isocitrate |