Values CatLog read from this paper
Substrate Specificity and Kinetic Mechanism of the NAD+-Dependent Deacylase from Mycobacterium tuberculosis, Rv1151c (Mt-Sirt)
PMID 42441379doi:10.1021/acs.biochem.6c00374Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 46 records (95 values) from this paper: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), isocitrate lyase 1 (MtICL1) from Mycobacterium tuberculosis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| isocitrate lyase 1 (MtICL1) | Mycobacterium tuberculosis | D-isocitrate | 22 | 0.051 | 430 | pH 7.5, 37 °C | Open record: isocitrate lyase 1 (MtICL1), D-isocitrate |
| isocitrate lyase 1 (MtICL1)variant K189E | Mycobacterium tuberculosis | D-isocitrate | 0.002 | 0.022 | 0.095 | pH 7.5, 37 °C | Open record: isocitrate lyase 1 (MtICL1) variant K189E, D-isocitrate |
| isocitrate lyase 1 (MtICL1)variant K322E | Mycobacterium tuberculosis | D-isocitrate | 38 | 0.097 | 390 | pH 7.5, 37 °C | Open record: isocitrate lyase 1 (MtICL1) variant K322E, D-isocitrate |
| isocitrate lyase 1 (MtICL1)variant K334E | Mycobacterium tuberculosis | D-isocitrate | 21 | 0.057 | 370 | pH 7.5, 37 °C | Open record: isocitrate lyase 1 (MtICL1) variant K334E, D-isocitrate |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH | — | 0.03 | — | pH 7.5, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHNAD+ fixed at 1 mM; apparent Km | — | 0.057 | — | pH 7.5, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHNAD+ fixed at 1 mM; apparent Km | — | 0.062 | — | pH 7.5, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHNAD+ fixed at 1 mM; apparent Km | — | 0.046 | — | pH 7.5, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHNAD+ fixed at 1 mM; apparent Km | — | 0.14 | — | pH 7.5, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH | — | 0.11 | — | pH 8, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | carbobenzyloxy-(Nε-acetyl)lysine-7-amino-4-methylcoumarin | — | — | 0.062 | pH 8.5 | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), carbobenzyloxy-(Nε-acetyl)lysine-7-amino-4-methylcoumarin |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | carbobenzyloxy-(Nε-propionyl)lysine-7-amino-4-methylcoumarin | — | — | 0.017 | pH 8.5 | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), carbobenzyloxy-(Nε-propionyl)lysine-7-amino-4-methylcoumarin |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) | Mycobacterium tuberculosis | carbobenzyloxy-(Nε-succinyl)lysine-7-amino-4-methylcoumarin | — | — | 0.59 | pH 8.5 | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase), carbobenzyloxy-(Nε-succinyl)lysine-7-amino-4-methylcoumarin |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase)variant R56M | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH | 0.1 | 0.2 | 0.5 | pH 8, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) variant R56M, Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase)variant Y53F | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OHTable 2 | 0.058 | 1.9 | 0.03 | pH 8, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) variant Y53F, Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase)variant Y53F | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OHSupplementary Figure S4 | 0.022 | 0.63 | 0.035 calc. | pH 8, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) variant Y53F, Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH |
| Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase)variant Y53F | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH | 0.09 | 0.26 | 0.32 | pH 8, 37 °C | Open record: Mt-Sirt (Rv1151c, NAD+-dependent lysine deacylase) variant Y53F, Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-acetyl)-Ile-Trp-OH | 0.112 | 1.1 | 0.11 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-acetyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-butyryl)-Ile-Trp-OH | 0.017 | 1.3 | 0.013 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-butyryl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-crotonyl)-Ile-Trp-OH | 0.007 | 4 | 0.0017 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-crotonyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-decanoyl)-Ile-Trp-OH | 0.023 | 0.024 | 0.95 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-decanoyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-dodecanoyl)-Ile-Trp-OH | 0.047 | 0.042 | 1.1 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-dodecanoyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-glutaryl)-Ile-Trp-OH | 0.055 | 0.11 | 0.52 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-glutaryl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-hexanoyl)-Ile-Trp-OH | 0.017 | 0.1 | 0.017 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-hexanoyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-malonyl)-Ile-Trp-OH | 0.048 | 1.4 | 0.036 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-malonyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-myristoyl)-Ile-Trp-OH | 0.031 | 0.018 | 1.8 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-myristoyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-octanoyl)-Ile-Trp-OH | 0.036 | 0.18 | 0.19 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-octanoyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-palmitoyl)-Ile-Trp-OH | 0.016 | 0.008 | 2 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-palmitoyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH | 0.078 | 0.41 | 0.19 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHsaturating NAD+ (1 mM); variable acyl peptide (0-1.5 mM) | 0.157 | 0.042 | 3.8 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH | — | 0.03 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHproduct inhibition by nicotinamide (NAM, 0-400 µM) | — | 0.057 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHproduct inhibition by nicotinamide (NAM, 0-400 µM) | — | 0.062 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHdead-end inhibition by ADP-ribose (ADPR, 0-5000 µM) | — | 0.046 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHdead-end inhibition by Cbz-Lys-AMC (0-2000 µM); apparent Km | — | 0.14 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OHNAD+ fixed at 1 mM; HPLC assay | — | 0.11 | — | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | carbobenzyloxy-(Nε-acetyl)lysine-7-amino-4-methylcoumarin | 0.009 | 0.157 | 0.057 calc. | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), carbobenzyloxy-(Nε-acetyl)lysine-7-amino-4-methylcoumarin | |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | carbobenzyloxy-(Nε-succinyl)lysine-7-amino-4-methylcoumarin | 0.015 | 0.0165 | 0.91 calc. | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), carbobenzyloxy-(Nε-succinyl)lysine-7-amino-4-methylcoumarin | |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | NAD+product inhibition by nicotinamide (NAM, 0-800 µM) | — | 0.24 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), NAD+ |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | NAD+dead-end inhibition by ADP-ribose (ADPR, 0-5000 µM) | — | 0.26 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), NAD+ |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | NAD+dead-end inhibition by Cbz-Lys-AMC (0-2000 µM) | — | 0.77 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), NAD+ |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c) | Mycobacterium tuberculosis | NAD+dead-end inhibition by Cbz-Lys-AMC (0-2000 µM) | — | 0.12 | — | pH 7.5, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c), NAD+ |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c)variant R56M | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH | 0.1 | 0.2 | 0.5 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c) variant R56M, Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c)variant Y53F | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OHTable 2 | 0.058 | 1.9 | 0.03 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c) variant Y53F, Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c)variant Y53F | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OHin the text | 0.022 | — | — | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c) variant Y53F, Ac-Arg-Ser-Gly-Lys(Nε-propionyl)-Ile-Trp-OH |
| NAD+-dependent deacylase Mt-Sirt (Rv1151c)variant Y53F | Mycobacterium tuberculosis | Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH | 0.09 | 0.26 | 0.32 | pH 8, 37 °C | Open record: NAD+-dependent deacylase Mt-Sirt (Rv1151c) variant Y53F, Ac-Arg-Ser-Gly-Lys(Nε-succinyl)-Ile-Trp-OH |