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Catalytic residues and an electrostatic sandwich that promote enolpyruvyl shikimate 3-phosphate synthase (AroA) catalysis
PMID 19271774doi:10.1021/bi802251sOpen in Browse
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CatLog read 28 records (66 values) from this paper: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) from Escherichia coli.
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| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) | Escherichia coli | phosphoenolpyruvate50 mM KCl | — | 0.04 | 800 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase), phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) | Escherichia coli | phosphoenolpyruvate100 mM KCl | — | 0.15 | 90 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase), phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) | Escherichia coli | phosphoenolpyruvate100 mM KCl | — | 0.16 | 86 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase), phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) | Escherichia coli | shikimate 3-phosphate50 mM KCl | 32 | 0.018 | 1.8 × 10³ | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase), shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) | Escherichia coli | shikimate 3-phosphate100 mM KCl | 13 | 0.11 | 150 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase), shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) | Escherichia coli | shikimate 3-phosphate100 mM KCl | 14 | 0.14 | 96 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase), shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant D313A | Escherichia coli | phosphoenolpyruvate | — | 0.28 | 0.004 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant D313A, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant D313A | Escherichia coli | shikimate 3-phosphate | 0.0012 | 0.21 | 0.006 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant D313A, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant D313C | Escherichia coli | phosphoenolpyruvate | — | 1.3 | 0.02 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant D313C, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant D313C | Escherichia coli | shikimate 3-phosphate | 0.026 | 0.4 | 0.068 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant D313C, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant D313L | Escherichia coli | phosphoenolpyruvate | — | 1.6 | 7 × 10⁻⁴ | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant D313L, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant D313L | Escherichia coli | shikimate 3-phosphate | 0.0011 | 0.7 | 0.0016 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant D313L, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant D313N | Escherichia coli | phosphoenolpyruvate | — | 0.19 | 0.01 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant D313N, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant D313N | Escherichia coli | shikimate 3-phosphate | 0.0018 | 0.2 | 0.009 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant D313N, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant E341A | Escherichia coli | phosphoenolpyruvate | — | 0.05 | 0.009 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant E341A, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant E341A | Escherichia coli | shikimate 3-phosphate | 4.2 × 10⁻⁴ | 0.1 | 0.005 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant E341A, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant E341C | Escherichia coli | phosphoenolpyruvate | — | 0.17 | 0.021 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant E341C, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant E341C | Escherichia coli | shikimate 3-phosphate | 0.0036 | 0.08 | 0.044 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant E341C, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant E341M | Escherichia coli | phosphoenolpyruvate | — | 1.9 | 9 × 10⁻⁴ | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant E341M, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant E341M | Escherichia coli | shikimate 3-phosphate | 0.0017 | 0.035 | 0.05 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant E341M, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant E341Q | Escherichia coli | phosphoenolpyruvate | — | 0.9 | 0.0013 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant E341Q, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant E341Q | Escherichia coli | shikimate 3-phosphate | — | 0.04 | 0.027 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant E341Q, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant H385A | Escherichia coli | phosphoenolpyruvate | — | 1.5 | 0.3 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant H385A, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant H385A | Escherichia coli | shikimate 3-phosphate | 0.41 | 0.09 | 4 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant H385A, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant K22A | Escherichia coli | phosphoenolpyruvate | — | — | 0.009 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant K22A, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant K22A | Escherichia coli | shikimate 3-phosphate | — | 2 | — | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant K22A, shikimate 3-phosphate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant K22R | Escherichia coli | phosphoenolpyruvate | — | 2.5 | 1.2 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant K22R, phosphoenolpyruvate |
| enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase)variant K22R | Escherichia coli | shikimate 3-phosphate | 3 | 0.19 | 16 | pH 7.5, 25 °C | Open record: enolpyruvyl shikimate 3-phosphate synthase (AroA, EPSP synthase) variant K22R, shikimate 3-phosphate |