Values CatLog read from this paper
Purification and characterization of a monomeric isocitrate dehydrogenase with dual coenzyme specificity from the photosynthetic bacterium Rhodomicrobium vannielii
PMID 1935983doi:10.1111/j.1432-1033.1991.tb16347.xOpen in Browse
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CatLog read 8 records (16 values) from this paper: isocitrate dehydrogenase (dual coenzyme specific, monomeric) from Rhodomicrobium vannielii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| isocitrate dehydrogenase (dual coenzyme specific, monomeric) | Rhodomicrobium vannielii | nicotinamide adenine dinucleotidestandard assay: 20 mM Tris/HCl pH 8.0; NAD(P)H at 340 nm | 31.5 | 0.77 | 40.92 | pH 8, 30 °C | Open record: isocitrate dehydrogenase (dual coenzyme specific, monomeric), nicotinamide adenine dinucleotide |
| isocitrate dehydrogenase (dual coenzyme specific, monomeric) | Rhodomicrobium vannielii | nicotinamide adenine dinucleotide20 mM Tris/HCl pH 8.0; 2 mM MgCl2 | 29.75 | 3.6 | 8.27 | pH 8, 30 °C | Open record: isocitrate dehydrogenase (dual coenzyme specific, monomeric), nicotinamide adenine dinucleotide |
| isocitrate dehydrogenase (dual coenzyme specific, monomeric) | Rhodomicrobium vannielii | nicotinamide adenine dinucleotide20 mM phosphate pH 8.0; 2 mM MgCl2 | 12.03 | 1.8 | 6.68 | pH 8, 30 °C | Open record: isocitrate dehydrogenase (dual coenzyme specific, monomeric), nicotinamide adenine dinucleotide |
| isocitrate dehydrogenase (dual coenzyme specific, monomeric) | Rhodomicrobium vannielii | nicotinamide adenine dinucleotide phosphate | 40.88 | 0.0025 | 16,353 | pH 8, 30 °C | Open record: isocitrate dehydrogenase (dual coenzyme specific, monomeric), nicotinamide adenine dinucleotide phosphate |
| isocitrate dehydrogenase (dual coenzyme specific, monomeric) | Rhodomicrobium vannielii | thionicotinamide-adenine dinucleotide | — | 3.77 | — | pH 8 | Open record: isocitrate dehydrogenase (dual coenzyme specific, monomeric), thionicotinamide-adenine dinucleotide |
| isocitrate dehydrogenase (dual coenzyme specific, monomeric) | Rhodomicrobium vannielii | thionicotinamide-adenine dinucleotide phosphate | — | 0.026 | — | pH 8 | Open record: isocitrate dehydrogenase (dual coenzyme specific, monomeric), thionicotinamide-adenine dinucleotide phosphate |
| isocitrate dehydrogenase (dual coenzyme specific, monomeric) | Rhodomicrobium vannielii | threo-Ds-isocitrateNADP-linked reaction; cosubstrate NADP | — | 0.0033 | — | pH 8, 30 °C | Open record: isocitrate dehydrogenase (dual coenzyme specific, monomeric), threo-Ds-isocitrate |
| isocitrate dehydrogenase (dual coenzyme specific, monomeric) | Rhodomicrobium vannielii | threo-Ds-isocitrateNAD-linked reaction; cosubstrate NAD | — | 0.0085 | — | pH 8, 30 °C | Open record: isocitrate dehydrogenase (dual coenzyme specific, monomeric), threo-Ds-isocitrate |