Values CatLog read from this paper
Enzymatic properties of mutant Escherichia coli tryptophan synthase alpha-subunits
PMID 1939081doi:10.1016/s0021-9258(18)54910-3Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 88 records (249 values) from this paper: tryptophan synthase alpha2.beta2 complex, tryptophan synthase alpha subunit (alone, without beta2 subunit), tryptophan synthase beta2 subunit (alone, without alpha subunit) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tryptophan synthase alpha subunit (alone, without beta2 subunit) | Escherichia coli | indole-3-glycerol phosphate | 0.005 | 0.25 | 0.02 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha subunit (alone, without beta2 subunit), indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complex | Escherichia coli | indole | 9.54 | 0.03 | 318 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex, indole |
| tryptophan synthase alpha2.beta2 complex | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.48 | 0.46 | 1.04 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complex | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6.64 | 0.041 | 162 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant A67V | Escherichia coli | indole | 1.04 | 0.038 | 27 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant A67V, indole |
| tryptophan synthase alpha2.beta2 complexvariant A67V | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.047 | 0.51 | 0.09 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant A67V, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant A67V | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 0.7 | 0.15 | 4.7 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant A67V, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant C81R | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.233 | 0.23 | 1.01 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant C81R, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant C81R | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 3.83 | 0.028 | 136 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant C81R, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D112G | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.458 | 0.42 | 1.09 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D112G, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D112G | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 5.74 | 0.04 | 144 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D112G, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D112G (DG112) | Escherichia coli | indole-3-glycerol phosphate | — | — | 1.09 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D112G (DG112), indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D112N | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.473 | 0.44 | 1.07 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D112N, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D112N | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 5.52 | 0.035 | 158 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D112N, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D112N (DN112) | Escherichia coli | indole-3-glycerol phosphate | — | — | 1.07 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D112N (DN112), indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D46G | Escherichia coli | indole-3-glycerol phosphate | 5.8 | 0.03 | 193 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D46G, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D46N | Escherichia coli | indole-3-glycerol phosphate | 6.07 | 0.034 | 178 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D46N, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D56G | Escherichia coli | indole | 0.19 | 0.024 | 7.9 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D56G, indole |
| tryptophan synthase alpha2.beta2 complexvariant D56G | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.054 | 0.22 | 0.24 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D56G, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant D56G | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 0.09 | 0.074 | 1.2 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant D56G, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant F114L | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.311 | 0.31 | 1.01 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant F114L, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant F114L | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6.18 | 0.064 | 97 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant F114L, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant F22S | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.048 | 0.27 | 0.18 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant F22S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant F22S | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 0.48 | 0.596 | 0.8 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant F22S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant G44S | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.408 | 0.37 | 1.1 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant G44S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant G44S | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6.09 | 0.037 | 165 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant G44S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant G51D | Escherichia coli | indole | 4.29 | 0.018 | 238 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant G51D, indole |
| tryptophan synthase alpha2.beta2 complexvariant G51D | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.122 | — | 0.31 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant G51D, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant G51D | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 1.11 | 0.277 | 4 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant G51D, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant G51D (GD51) | Escherichia coli | indole-3-glycerol phosphate | — | 0.39 | — | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant G51D (GD51), indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant M101I | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.31 | 0.33 | 0.92 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant M101I, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant M101I | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 5.5 | 0.076 | 72 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant M101I, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant M101T | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.39 | 0.43 | 0.91 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant M101T, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant M101T | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 5.45 | 0.07 | 78 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant M101T, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant M101V | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.39 | 0.41 | 0.95 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant M101V, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant M101V | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 5.86 | 0.093 | 63 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant M101V, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant N104S | Escherichia coli | indole | 2.7 | 0.032 | 84 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant N104S, indole |
| tryptophan synthase alpha2.beta2 complexvariant N104S | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.451 | 0.49 | 0.92 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant N104S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant N104S | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 4.93 | 0.065 | 76 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant N104S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P21S | Escherichia coli | indole | 9.51 | 0.03 | 317.1 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P21S, indole |
| tryptophan synthase alpha2.beta2 complexvariant P21S | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.408 | — | 1.05 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P21S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P21S | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6.55 | 0.053 | 124 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P21S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P21S (PS21) | Escherichia coli | indole-3-glycerol phosphate | — | 0.39 | — | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P21S (PS21), indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P28L | Escherichia coli | indole | 6.72 | 0.021 | 320 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P28L, indole |
| tryptophan synthase alpha2.beta2 complexvariant P28L | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.222 | 0.26 | 0.85 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P28L, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P28L | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 3.61 | 0.024 | 150 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P28L, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P28S | Escherichia coli | indole | 9.47 | 0.034 | 278.6 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P28S, indole |
| tryptophan synthase alpha2.beta2 complexvariant P28S | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.419 | — | 0.95 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P28S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P28S | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6.12 | 0.039 | 157 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P28S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P53H | Escherichia coli | indole | 0.49 | 0.024 | 20 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P53H, indole |
| tryptophan synthase alpha2.beta2 complexvariant P53H | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.039 | 0.39 | 0.1 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P53H, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P53H | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 1.1 | 0.119 | 9.3 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P53H, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P53T | Escherichia coli | indole | 1.85 | 0.01 | 185 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P53T, indole |
| tryptophan synthase alpha2.beta2 complexvariant P53T | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.269 | 0.54 | 0.5 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P53T, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P53T | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 3.88 | 0.107 | 36.2 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P53T, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P62Q | Escherichia coli | indole | 8.31 | 0.021 | 396 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P62Q, indole |
| tryptophan synthase alpha2.beta2 complexvariant P62Q | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.465 | 0.47 | 0.99 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P62Q, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P62Q | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 4.88 | 0.029 | 168 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P62Q, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P78L | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.422 | 0.29 | 1.11 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P78L, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P78L | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6 | 0.059 | 102 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P78L, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P78S | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.451 | 0.42 | 1.07 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P78S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P78S | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6.05 | 0.041 | 148 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P78S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant P98S | Escherichia coli | indole | 9.04 | 0.026 | 350 calc. | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P98S, indole |
| tryptophan synthase alpha2.beta2 complexvariant P98S | Escherichia coli | indole-3-glycerol phosphate | 0.476 | 0.48 | 0.99 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant P98S, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Q65R | Escherichia coli | indole | 11.64 | 0.04 | 291 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Q65R, indole |
| tryptophan synthase alpha2.beta2 complexvariant Q65R | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.054 | 0.13 | 0.41 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Q65R, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Q65R | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 2.9 | 0.021 | 138 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Q65R, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant S33L | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.437 | 0.41 | 1.07 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant S33L, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant S33L | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 5.17 | 0.035 | 148 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant S33L, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant S6P | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.523 | 0.49 | 1.07 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant S6P, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant S6P | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6.2 | 0.031 | 200 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant S6P, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant T24M | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.58 | 0.98 | 1 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant T24M, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant T24M | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 6.49 | 0.049 | 132 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant T24M, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant T63K | Escherichia coli | indole | 9.84 | 0.032 | 307 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant T63K, indole |
| tryptophan synthase alpha2.beta2 complexvariant T63K | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.136 | 0.15 | 0.91 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant T63K, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant T63K | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 2.71 | 0.059 | 46 calc. | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant T63K, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y102C | Escherichia coli | indole | 0.76 | 0.04 | 19 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y102C, indole |
| tryptophan synthase alpha2.beta2 complexvariant Y102C | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.048 | 0.45 | 0.11 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y102C, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y102C | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 1.21 | 0.253 | 4.8 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y102C, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y102H | Escherichia coli | indole | 7.63 | 0.032 | 238 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y102H, indole |
| tryptophan synthase alpha2.beta2 complexvariant Y102H | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.49 | 1.3 | 0.34 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y102H, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y102H | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 4.62 | 0.124 | 37.4 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y102H, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y115C | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.458 | 0.42 | 1.09 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y115C, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y115C | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 5.47 | 0.041 | 133 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y115C, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y115C (YC115) | Escherichia coli | indole-3-glycerol phosphate | — | — | 1.09 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y115C (YC115), indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y4C | Escherichia coli | indole-3-glycerol phosphateno L-serine; InGP varied 0.05-0.2 mM | 0.333 | 0.39 | 0.85 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y4C, indole-3-glycerol phosphate |
| tryptophan synthase alpha2.beta2 complexvariant Y4C | Escherichia coli | indole-3-glycerol phosphateInGP varied 0.05-0.2 mM with 40 mM L-serine | 4.88 | 0.052 | 94 | pH 7.8, 37 °C | Open record: tryptophan synthase alpha2.beta2 complex variant Y4C, indole-3-glycerol phosphate |
| tryptophan synthase beta2 subunit (alone, without alpha subunit) | Escherichia coli | indole | 0.23 | 0.034 | 6.6 | pH 7.8, 37 °C | Open record: tryptophan synthase beta2 subunit (alone, without alpha subunit), indole |