Values CatLog read from this paper
Escherichia coli DNA adenine methyltransferase: the structural basis of processive catalysis and indirect read-out
PMID 19419959doi:10.1074/jbc.m109.005876Open in Browse
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CatLog read 13 records (39 values) from this paper: DNA adenine methyltransferase (EcoDam) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| DNA adenine methyltransferase (EcoDam) | Escherichia coli | non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ | 0.0152 | 8.1 × 10⁻⁵ | 187 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam), non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam) | Escherichia coli | preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ | 0.0038 | 2.3 × 10⁻⁵ | 170 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam), preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant K139A | Escherichia coli | non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ | 0.0082 | 2.7 × 10⁻⁵ | 300 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant K139A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant K139A | Escherichia coli | preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ | 0.0055 | 4.4 × 10⁻⁵ | 125 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant K139A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant N126A | Escherichia coli | preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ | 0.0015 | 3.1 × 10⁻⁵ | 48 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant N126A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant N132A | Escherichia coli | non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ | 0.0017 | 1 × 10⁻⁵ | 160 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant N132A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant N132A | Escherichia coli | preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ | 0.02 | 7.6 × 10⁻⁵ | 270 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant N132A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant R116A | Escherichia coli | non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ | 9 × 10⁻⁴ | 7 × 10⁻⁶ | 130 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant R116A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant R116A | Escherichia coli | preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ | 0.013 | 3.5 × 10⁻⁵ | 370 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant R116A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant R137A | Escherichia coli | non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ | 0.058 | 2.2 × 10⁻⁴ | 262 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant R137A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant R137A | Escherichia coli | preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ | 0.0187 | 8.3 × 10⁻⁵ | 223 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant R137A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant R95A | Escherichia coli | non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ | 8.2 × 10⁻⁴ | 8 × 10⁻⁶ | 120 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant R95A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′ |
| DNA adenine methyltransferase (EcoDam)variant R95A | Escherichia coli | preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ | 0.0083 | 8.6 × 10⁻⁵ | 100 | pH 8, 22 °C | Open record: DNA adenine methyltransferase (EcoDam) variant R95A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′ |