EC 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific)

TransferasesOpen in Browse

CatLog read 33 records for EC 2.1.1.72 from 3 papers, for 2 organisms. Names in these papers: DNA adenine methyltransferase (EcoDam), M.EcoVIII (N6-adenine DNA methyltransferase), CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 20 records for EC 2.1.1.72 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.1.1.72 in Browse.

Papers

Papers with records for EC 2.1.1.72
Paper	Organisms	Records
Escherichia coli DNA adenine methyltransferase: the structural basis of processive catalysis and indirect read-out	Escherichia coli	13
Isospecific adenine DNA methyltransferases show distinct preferences towards DNA substrates	Escherichia coli	13
Protein loops are major contributors to DNA strand separation and high-fidelity substrate recognition for DNA methyltransferase CcrM	Caulobacter crescentus	7

Records

Records for EC 2.1.1.72 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase)	Caulobacter crescentus	cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site	0.15	0.0011	130	pH 8, 22 °C	PMID 41887600	Open record: CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase), cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site
CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase)variant F125A	Caulobacter crescentus	cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site	2 × 10⁻⁴	0.0059	0.034	pH 8, 22 °C	PMID 41887600	Open record: CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase) variant F125A, cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site
CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase)variant F125L	Caulobacter crescentus	cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site	0.006	0.0021	3	pH 8, 22 °C	PMID 41887600	Open record: CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase) variant F125L, cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site
CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase)variant F125W	Caulobacter crescentus	cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site	0.01	0.0011	10	pH 8, 22 °C	PMID 41887600	Open record: CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase) variant F125W, cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site
CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase)variant N124A	Caulobacter crescentus	cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site	2.7 × 10⁻⁴	0.0017	0.16	pH 8, 22 °C	PMID 41887600	Open record: CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase) variant N124A, cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site
CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase)variant R129A	Caulobacter crescentus	cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site	3.5 × 10⁻⁵	0.0014	0.025	pH 8, 22 °C	PMID 41887600	Open record: CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase) variant R129A, cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site
CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase)variant R44A	Caulobacter crescentus	cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site	5 × 10⁻⁵	0.0018	0.027	pH 8, 22 °C	PMID 41887600	Open record: CcrM (cell cycle-regulated DNA methyltransferase, N6-adenine DNA methyltransferase) variant R44A, cognate hemimethylated double-stranded DNA with the 5'-GANTC-3' recognition site
DNA adenine methyltransferase (EcoDam)	Escherichia coli	non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′	0.0152	8.1 × 10⁻⁵	187	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam), non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′
DNA adenine methyltransferase (EcoDam)	Escherichia coli	preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′	0.0038	2.3 × 10⁻⁵	170	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam), preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant K139A	Escherichia coli	non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′	0.0082	2.7 × 10⁻⁵	300	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant K139A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant K139A	Escherichia coli	preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′	0.0055	4.4 × 10⁻⁵	125	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant K139A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant N126A	Escherichia coli	preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′	0.0015	3.1 × 10⁻⁵	48	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant N126A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant N132A	Escherichia coli	non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′	0.0017	1 × 10⁻⁵	160	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant N132A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant N132A	Escherichia coli	preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′	0.02	7.6 × 10⁻⁵	270	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant N132A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant R116A	Escherichia coli	non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′	9 × 10⁻⁴	7 × 10⁻⁶	130	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant R116A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant R116A	Escherichia coli	preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′	0.013	3.5 × 10⁻⁵	370	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant R116A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant R137A	Escherichia coli	non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′	0.058	2.2 × 10⁻⁴	262	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant R137A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant R137A	Escherichia coli	preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′	0.0187	8.3 × 10⁻⁵	223	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant R137A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant R95A	Escherichia coli	non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′	8.2 × 10⁻⁴	8 × 10⁻⁶	120	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant R95A, non-preferred 21-bp GATC duplex DNA with a 5′ A-tract: 5′-CATTTAGACGATCTTTTATGC-3′ annealed to 5′-GCATAAAAGATCGTCTAAATG-3′
DNA adenine methyltransferase (EcoDam)variant R95A	Escherichia coli	preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′	0.0083	8.6 × 10⁻⁵	100	pH 8, 22 °C	PMID 19419959	Open record: DNA adenine methyltransferase (EcoDam) variant R95A, preferred 21-bp GATC duplex DNA: 5′-CATTTACTTGATCCGGTATGC-3′ annealed to 5′-GCATACCGGATCAAGTAAATG-3′
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AAACTT1/AAACTT2 (secondary site 5′-AAACTT-3′, G3→A)	3.3 × 10⁻⁴	1.023 × 10⁻⁴	1.7	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AAACTT1/AAACTT2 (secondary site 5′-AAACTT-3′, G3→A)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AACCTT1/AACCTT2 (secondary site 5′-AACCTT-3′, G3→C)	2.7 × 10⁻⁴	1.273 × 10⁻⁴	1.9	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AACCTT1/AACCTT2 (secondary site 5′-AACCTT-3′, G3→C)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AAGCTT1/AAGCTT2 (unmethylated 5′-AAGCTT-3′ target site)	0.00147	7.51 × 10⁻⁵	19.6	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AAGCTT1/AAGCTT2 (unmethylated 5′-AAGCTT-3′ target site)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AATCTT1/AATCTT2 (secondary site 5′-AATCTT-3′, G3→T)	3.3 × 10⁻⁴	1.43 × 10⁻⁴	2.9	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AATCTT1/AATCTT2 (secondary site 5′-AATCTT-3′, G3→T)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo ACGCTT1/ACGCTT2 (secondary site 5′-ACGCTT-3′, A2→C)	2.2 × 10⁻⁴	8.9 × 10⁻⁵	2.6	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo ACGCTT1/ACGCTT2 (secondary site 5′-ACGCTT-3′, A2→C)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AGGCTT1/AGGCTT2 (secondary site 5′-AGGCTT-3′, A2→G)	2 × 10⁻⁴	9.12 × 10⁻⁵	1.7	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AGGCTT1/AGGCTT2 (secondary site 5′-AGGCTT-3′, A2→G)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo ATGCTT1/ATGCTT2 (secondary site 5′-ATGCTT-3′, A2→T)	2.5 × 10⁻⁴	9.14 × 10⁻⁵	2.7	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo ATGCTT1/ATGCTT2 (secondary site 5′-ATGCTT-3′, A2→T)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo CAGCTT1/CAGCTT2 (secondary site 5′-CAGCTT-3′, A1→C)	3.3 × 10⁻⁴	7.82 × 10⁻⁵	4.2	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo CAGCTT1/CAGCTT2 (secondary site 5′-CAGCTT-3′, A1→C)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo GAGCTT1/GAGCTT2 (secondary site 5′-GAGCTT-3′, A1→G)	3.3 × 10⁻⁴	7.84 × 10⁻⁵	4.3	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo GAGCTT1/GAGCTT2 (secondary site 5′-GAGCTT-3′, A1→G)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo MAGCTT/AAGCTT2 (hemimethylated 5′-AAGCTT-3′ target site, M = N6-methyladenine)	0.0021	2.44 × 10⁻⁵	86.1	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo MAGCTT/AAGCTT2 (hemimethylated 5′-AAGCTT-3′ target site, M = N6-methyladenine)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo TAGCTT1/TAGCTT2 (secondary site 5′-TAGCTT-3′, A1→T)	2.2 × 10⁻⁴	8.55 × 10⁻⁵	2.2	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo TAGCTT1/TAGCTT2 (secondary site 5′-TAGCTT-3′, A1→T)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	S-adenosyl-L-methionine	—	2.7 × 10⁻⁴	—	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), S-adenosyl-L-methionine
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ss oligo AAGCTT1 (single-stranded DNA, 5′-AAGCTT-3′ target site)	5 × 10⁻⁴	2.576 × 10⁻⁴	1.9	pH 7, 37 °C	PMID 29844340	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ss oligo AAGCTT1 (single-stranded DNA, 5′-AAGCTT-3′ target site)