Values CatLog read from this paper

Isospecific adenine DNA methyltransferases show distinct preferences towards DNA substrates

PMID 29844340doi:10.1038/s41598-018-26434-0Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 13 records (37 values) from this paper: M.EcoVIII (N6-adenine DNA methyltransferase) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.1.1.72

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AAACTT1/AAACTT2 (secondary site 5′-AAACTT-3′, G3→A)	3.3 × 10⁻⁴	1.023 × 10⁻⁴	1.7	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AAACTT1/AAACTT2 (secondary site 5′-AAACTT-3′, G3→A)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AACCTT1/AACCTT2 (secondary site 5′-AACCTT-3′, G3→C)	2.7 × 10⁻⁴	1.273 × 10⁻⁴	1.9	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AACCTT1/AACCTT2 (secondary site 5′-AACCTT-3′, G3→C)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AAGCTT1/AAGCTT2 (unmethylated 5′-AAGCTT-3′ target site)	0.00147	7.51 × 10⁻⁵	19.6	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AAGCTT1/AAGCTT2 (unmethylated 5′-AAGCTT-3′ target site)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AATCTT1/AATCTT2 (secondary site 5′-AATCTT-3′, G3→T)	3.3 × 10⁻⁴	1.43 × 10⁻⁴	2.9	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AATCTT1/AATCTT2 (secondary site 5′-AATCTT-3′, G3→T)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo ACGCTT1/ACGCTT2 (secondary site 5′-ACGCTT-3′, A2→C)	2.2 × 10⁻⁴	8.9 × 10⁻⁵	2.6	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo ACGCTT1/ACGCTT2 (secondary site 5′-ACGCTT-3′, A2→C)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo AGGCTT1/AGGCTT2 (secondary site 5′-AGGCTT-3′, A2→G)	2 × 10⁻⁴	9.12 × 10⁻⁵	1.7	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo AGGCTT1/AGGCTT2 (secondary site 5′-AGGCTT-3′, A2→G)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo ATGCTT1/ATGCTT2 (secondary site 5′-ATGCTT-3′, A2→T)	2.5 × 10⁻⁴	9.14 × 10⁻⁵	2.7	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo ATGCTT1/ATGCTT2 (secondary site 5′-ATGCTT-3′, A2→T)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo CAGCTT1/CAGCTT2 (secondary site 5′-CAGCTT-3′, A1→C)	3.3 × 10⁻⁴	7.82 × 10⁻⁵	4.2	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo CAGCTT1/CAGCTT2 (secondary site 5′-CAGCTT-3′, A1→C)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo GAGCTT1/GAGCTT2 (secondary site 5′-GAGCTT-3′, A1→G)	3.3 × 10⁻⁴	7.84 × 10⁻⁵	4.3	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo GAGCTT1/GAGCTT2 (secondary site 5′-GAGCTT-3′, A1→G)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo MAGCTT/AAGCTT2 (hemimethylated 5′-AAGCTT-3′ target site, M = N6-methyladenine)	0.0021	2.44 × 10⁻⁵	86.1	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo MAGCTT/AAGCTT2 (hemimethylated 5′-AAGCTT-3′ target site, M = N6-methyladenine)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ds oligo TAGCTT1/TAGCTT2 (secondary site 5′-TAGCTT-3′, A1→T)	2.2 × 10⁻⁴	8.55 × 10⁻⁵	2.2	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ds oligo TAGCTT1/TAGCTT2 (secondary site 5′-TAGCTT-3′, A1→T)
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	S-adenosyl-L-methionine	—	2.7 × 10⁻⁴	—	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), S-adenosyl-L-methionine
M.EcoVIII (N6-adenine DNA methyltransferase)	Escherichia coli	ss oligo AAGCTT1 (single-stranded DNA, 5′-AAGCTT-3′ target site)	5 × 10⁻⁴	2.576 × 10⁻⁴	1.9	pH 7, 37 °C	Open record: M.EcoVIII (N6-adenine DNA methyltransferase), ss oligo AAGCTT1 (single-stranded DNA, 5′-AAGCTT-3′ target site)