Values CatLog read from this paper
Characterization of an aldolase-dehydrogenase complex that exhibits substrate channeling in the polychlorinated biphenyls degradation pathway
PMID 19476337doi:10.1021/bi9006644Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (45 values) from this paper: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), BphJ (acylating aldehyde dehydrogenase) from Burkholderia xenovorans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxoheptanoate | 0.63 | 0.35 | 1.8 | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxoheptanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxohexanoate | 3.8 | 0.18 | 21 | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxohexanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate | 4.1 | 0.22 | 19 | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoatestandard continuous assay; coupled assay: 0.4 mM NADH | 4.6 | 0.25 | 18 calc. | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate80 µg enzyme; 1 mM MnCl2; 100 mM sodium HEPES buffer | 0.87 | 1.12 | 0.78 | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate80 µg enzyme; 1 mM MnCl2; 100 mM sodium HEPES buffer | 0.78 | 0.15 | 5.25 | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate80 µg enzyme; 1 mM MnCl2; 100 mM sodium HEPES buffer | 1.75 | 0.23 | 7.65 | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate80 µg enzyme; 1 mM MnCl2; 100 mM sodium HEPES buffer | 0.66 | 0.11 | 5.9 | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | oxaloacetate | 16.5 | 7.5 | 2.2 | pH 8, 25 °C | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), oxaloacetate |
| BphJ (acylating aldehyde dehydrogenase) | Burkholderia xenovorans | acetaldehyde | 17.2 | 23.6 | 0.73 | pH 8, 25 °C | Open record: BphJ (acylating aldehyde dehydrogenase), acetaldehyde |
| BphJ (acylating aldehyde dehydrogenase) | Burkholderia xenovorans | butyraldehyde | 9.5 | 31.7 | 0.3 | pH 8, 25 °C | Open record: BphJ (acylating aldehyde dehydrogenase), butyraldehyde |
| BphJ (acylating aldehyde dehydrogenase) | Burkholderia xenovorans | isobutyraldehyde | 11.2 | 7.7 | 1.4 | pH 8, 25 °C | Open record: BphJ (acylating aldehyde dehydrogenase), isobutyraldehyde |
| BphJ (acylating aldehyde dehydrogenase) | Burkholderia xenovorans | NAD+ | 14.1 | 0.0348 | 400 | pH 8, 25 °C | Open record: BphJ (acylating aldehyde dehydrogenase), NAD+ |
| BphJ (acylating aldehyde dehydrogenase) | Burkholderia xenovorans | NADP+ | 12.9 | 0.561 | 22 | pH 8, 25 °C | Open record: BphJ (acylating aldehyde dehydrogenase), NADP+ |
| BphJ (acylating aldehyde dehydrogenase) | Burkholderia xenovorans | pentaldehyde | — | — | 0.076 | pH 8, 25 °C | Open record: BphJ (acylating aldehyde dehydrogenase), pentaldehyde |
| BphJ (acylating aldehyde dehydrogenase) | Burkholderia xenovorans | propionaldehyde | 16.3 | 23.1 | 0.7 | pH 8, 25 °C | Open record: BphJ (acylating aldehyde dehydrogenase), propionaldehyde |