EC 4.1.3.39: 4-hydroxy-2-oxovalerate aldolase
LyasesOpen in Browse
CatLog read 28 records for EC 4.1.3.39 from 4 papers, for 3 organisms. Names in these papers: aldolase TTHB246, BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), HsaF.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 1 record for EC 4.1.3.39 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.3.39 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Protein-protein interactions and substrate channeling in orthologous and chimeric aldolase-dehydrogenase complexes | Thermus thermophilus | 13 |
| Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling | Burkholderia xenovorans | 8 |
| Characterization of an aldolase-dehydrogenase complex that exhibits substrate channeling in the polychlorinated biphenyls degradation pathway | Burkholderia xenovorans | 5 |
| Characterization of an aldolase-dehydrogenase complex from the cholesterol degradation pathway of Mycobacterium tuberculosis | Mycobacterium tuberculosis | 2 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxohexanoateTTHB246 alone (uncomplexed) | 1.1 | 0.21 | 5.2 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxohexanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxohexanoateTTHB246-TTHB247 complex; in complex with TTHB247 | 0.5 | 0.037 | 13 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxohexanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxohexanoateTTHB246-BphJ complex; in complex with BphJ | 2.2 | 0.218 | 10 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxohexanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate1 mM CoCl2; TTHB246 alone (uncomplexed); Table 4 | 1 | 0.206 | 5 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate1 mM CoCl2; TTHB246-TTHB247 complex; in complex with TTHB247 | 0.6 | 0.041 | 14 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate1 mM CoCl2; TTHB246-BphJ complex; in complex with BphJ | 2.4 | 0.257 | 9.4 | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoateTTHB246-TTHB247 complex; in complex with TTHB247 | 0.3 | 0.123 | 2.9 | pH 8 | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoateTTHB246-BphJ complex; in complex with BphJ | 2.4 | 0.253 | 9.3 | pH 8 | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 1 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 0.79 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 0.57 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 0.13 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| aldolase TTHB246 | Thermus thermophilus | 4-hydroxy-2-oxopentanoate2 mM HOPA fixed; 30 μg enzyme; TTHB246 alone (uncomplexed) | 0.08 | — | — | pH 8, 25 °C | PMID 22316175 | Open record: aldolase TTHB246, 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoatestandard continuous assay; coupled assay: 0.4 mM NADH | 4.6 | 0.25 | 18 calc. | pH 8, 25 °C | PMID 19476337 | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate80 µg enzyme; 1 mM MnCl2; 100 mM sodium HEPES buffer | 0.87 | 1.12 | 0.78 | pH 8, 25 °C | PMID 19476337 | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate80 µg enzyme; 1 mM MnCl2; 100 mM sodium HEPES buffer | 0.78 | 0.15 | 5.25 | pH 8, 25 °C | PMID 19476337 | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate80 µg enzyme; 1 mM MnCl2; 100 mM sodium HEPES buffer | 1.75 | 0.23 | 7.65 | pH 8, 25 °C | PMID 19476337 | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase) | Burkholderia xenovorans | 4-hydroxy-2-oxopentanoate80 µg enzyme; 1 mM MnCl2; 100 mM sodium HEPES buffer | 0.66 | 0.11 | 5.9 | pH 8, 25 °C | PMID 19476337 | Open record: BphI (4-hydroxy-2-oxopentanoate pyruvate aldolase), 4-hydroxy-2-oxopentanoate |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | acetaldehyde100 mM pyruvate fixed; aldehyde varied; discontinuous assay | 0.86 | 64.28 | 0.0134 | pH 8, 25 °C | PMID 20364820 | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), acetaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | acetaldehydepyruvate (A) and acetaldehyde (B) both varied | — | 67 | — | pH 8, 25 °C | PMID 20364820 | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), acetaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | butyraldehyde | — | — | 0.00219 | pH 8, 25 °C | PMID 20364820 | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), butyraldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | glycolaldehyde | 0.4 | 124.6 | 0.0037 | pH 8, 25 °C | PMID 20364820 | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), glycolaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | isobutyraldehyde | — | — | 5 × 10⁻⁴ | pH 8, 25 °C | PMID 20364820 | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), isobutyraldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | pentaldehyde | — | — | 4.7 × 10⁻⁴ | pH 8, 25 °C | PMID 20364820 | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), pentaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | propionaldehyde | 1.79 | 135.9 | 0.0132 | pH 8, 25 °C | PMID 20364820 | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), propionaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | pyruvate | 1.04 | 14.8 | 0.0703 calc. | pH 8, 25 °C | PMID 20364820 | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), pyruvate |
| HsaF | Mycobacterium tuberculosis | 4(S)-4-hydroxy-2-oxopentanoate | 0.41 | 0.0044 | 93.1 | pH 8, 25 °C | PMID 23614353 | Open record: HsaF, 4(S)-4-hydroxy-2-oxopentanoate |
| HsaF | Mycobacterium tuberculosis | 4-hydroxy-2-oxohexanoate | 0.38 | 0.0048 | 79.1 | pH 8, 25 °C | PMID 23614353 | Open record: HsaF, 4-hydroxy-2-oxohexanoate |