Values CatLog read from this paper
Comparison of two metal-dependent pyruvate aldolases related by convergent evolution: substrate specificity, kinetic mechanism, and substrate channeling
PMID 20364820doi:10.1021/bi100251uOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (43 values) from this paper: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) from Escherichia coli, Burkholderia xenovorans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | acetaldehyde100 mM pyruvate fixed; aldehyde varied; discontinuous assay | 0.86 | 64.28 | 0.0134 | pH 8, 25 °C | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), acetaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | acetaldehydepyruvate (A) and acetaldehyde (B) both varied | — | 67 | — | pH 8, 25 °C | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), acetaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | butyraldehyde | — | — | 0.00219 | pH 8, 25 °C | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), butyraldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | glycolaldehyde | 0.4 | 124.6 | 0.0037 | pH 8, 25 °C | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), glycolaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | isobutyraldehyde | — | — | 5 × 10⁻⁴ | pH 8, 25 °C | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), isobutyraldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | pentaldehyde | — | — | 4.7 × 10⁻⁴ | pH 8, 25 °C | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), pentaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | propionaldehyde | 1.79 | 135.9 | 0.0132 | pH 8, 25 °C | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), propionaldehyde |
| BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase) | Burkholderia xenovorans | pyruvate | 1.04 | 14.8 | 0.0703 calc. | pH 8, 25 °C | Open record: BphI, 4-hydroxy-2-oxopentanoate aldolase (class II pyruvate aldolase), pyruvate |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | (D,L)-glyceraldehyde | 5.6 | 88.6 | 0.063 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), (D,L)-glyceraldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | acetaldehyde100 mM pyruvate fixed; aldehyde varied; discontinuous assay | 205.4 | 62.9 | 3.3 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), acetaldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | acetaldehyde2-ketobutanoate as carbonyl donor (fixed) | 21.9 | 50.1 | 0.4 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), acetaldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | acetaldehydepyruvate (A) and acetaldehyde (B) both varied | — | 62.1 | — | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), acetaldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | butyraldehyde | 132.5 | 13.4 | 9.9 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), butyraldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | glycolaldehyde | 175.5 | 33.3 | 5.27 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), glycolaldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | isobutyraldehyde | 64.9 | 73.8 | 0.9 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), isobutyraldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | pentaldehyde | — | — | 20 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), pentaldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | propionaldehyde | 358.4 | 32.9 | 10.9 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), propionaldehyde |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | pyruvate | 219.5 | 5.6 | 39 calc. | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), pyruvate |
| HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase) | Escherichia coli | succinic semialdehyde | 203.8 | 9.1 | 22.5 | pH 8, 25 °C | Open record: HpaI (HpcH), 4-hydroxy-2-oxo-1,7-heptanedioate aldolase (class II pyruvate aldolase), succinic semialdehyde |