Values CatLog read from this paper
ADP-dependent 6-phosphofructokinase from Pyrococcus horikoshii OT3: structure determination and biochemical characterization of PH1645
PMID 19553681doi:10.1074/jbc.m109.012401Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 30 records (74 values) from this paper: ADP-dependent 6-phosphofructokinase (PhPFK) from Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ADP-dependent 6-phosphofructokinase (PhPFK) | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0132 | 3.45 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK), adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK) | Pyrococcus horikoshii | D-fructose 6-phosphate | 45.5 | 0.0152 | 2.99 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK), D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant A71E | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0136 | 2.82 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant A71E, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant A71E | Pyrococcus horikoshii | D-fructose 6-phosphate | 39.3 | — | 1.72 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant A71E, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant A71E | Pyrococcus horikoshii | D-glucose | 2.68 | 3.95 | 0.678 calc. | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant A71E, D-glucose |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant D17A | Pyrococcus horikoshii | D-fructose 6-phosphate | 1.98 | 22.6 | 0.0876 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant D17A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant D433A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0139 | 0.345 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant D433A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant D433A | Pyrococcus horikoshii | D-fructose 6-phosphate | 0.0048 | 0.0127 | 0.378 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant D433A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant K158A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0379 | 1.08 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant K158A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant K158A | Pyrococcus horikoshii | D-fructose 6-phosphate | 41 | 6.5 | 6.31 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant K158A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant N15A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0238 | 3.83 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant N15A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant N15A | Pyrococcus horikoshii | D-fructose 6-phosphate | 91.1 | 0.103 | 884 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant N15A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant N160A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0724 | 2.09 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant N160A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant N160A | Pyrococcus horikoshii | D-fructose 6-phosphate | 151 | 0.415 | 364 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant N160A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant Q224A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0295 | 2.21 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant Q224A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant Q224A | Pyrococcus horikoshii | D-fructose 6-phosphate | 65.3 | 0.0386 | 1.69 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant Q224A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant Q97A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.00747 | 1.74 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant Q97A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant Q97A | Pyrococcus horikoshii | D-fructose 6-phosphate | 13 | 0.00708 | 1.84 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant Q97A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant R185A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.473 | 0.742 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant R185A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant R185A | Pyrococcus horikoshii | D-fructose 6-phosphate | 0.351 | 0.318 | 1.1 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant R185A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant R191A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0145 | 1.89 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant R191A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant R191A | Pyrococcus horikoshii | D-fructose 6-phosphate | 27.4 | 0.2544 | 107 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant R191A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant R191E | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0605 | 702 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant R191E, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant R191E | Pyrococcus horikoshii | D-fructose 6-phosphate | 42.5 | 4.87 | 8.73 | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant R191E, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant S183E | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0296 | 3.1 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant S183E, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant S183E | Pyrococcus horikoshii | D-fructose 6-phosphate | 91.7 | 0.0865 | 1.06 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant S183E, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant S189A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.016 | 1.6 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant S189A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant S189A | Pyrococcus horikoshii | D-fructose 6-phosphate | 25.5 | 0.00902 | 2.83 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant S189A, D-fructose 6-phosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant S263A | Pyrococcus horikoshii | adenosine 5'-diphosphate | — | 0.0208 | 3.08 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant S263A, adenosine 5'-diphosphate |
| ADP-dependent 6-phosphofructokinase (PhPFK)variant S263A | Pyrococcus horikoshii | D-fructose 6-phosphate | 64.1 | 0.0395 | 1.62 × 10³ | 50 °C | Open record: ADP-dependent 6-phosphofructokinase (PhPFK) variant S263A, D-fructose 6-phosphate |