CatLogenzyme kinetics catalog

EC 2.7.1.146: ADP-specific phosphofructokinase

TransferasesOpen in Browse

CatLog read 57 records for EC 2.7.1.146 from 6 papers, for 6 organisms. Names in these papers: ADP-dependent 6-phosphofructokinase (PhPFK), ADP-dependent phosphofructokinase (phPFK), ADP-dependent phosphofructokinase/glucokinase (MmazPFK/GK), bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) and 7 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 13 records for EC 2.7.1.146 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.7.1.146 in Browse.

Papers

PaperOrganismsRecords
ADP-dependent 6-phosphofructokinase from Pyrococcus horikoshii OT3: structure determination and biochemical characterization of PH1645Pyrococcus horikoshii30
Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinasesMethanococcus jannaschii, Pyrococcus horikoshii9
ADP-Dependent Kinases From the Archaeal Order Methanosarcinales Adapt to Salt by a Non-canonical Evolutionarily Conserved StrategyMethanosarcina mazei6
Catalytic and regulatory roles of divalent metal cations on the phosphoryl-transfer mechanism of ADP-dependent sugar kinases from hyperthermophilic archaeaPyrococcus horikoshii4
Bifunctional ADP-dependent phosphofructokinase/glucokinase activity in the order Methanococcales--biochemical characterization of the mesophilic enzyme from Methanococcus maripaludisMethanococcus maripaludis4
ADP-dependent phosphofructokinases from the archaeal order Methanosarcinales display redundant glucokinase activityMethanococcoides burtonii, Methanohalobium evestigatum4

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
ADP-dependent 6-phosphofructokinase (PhPFK)Pyrococcus horikoshiiadenosine 5'-diphosphate—0.01323.45 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)Pyrococcus horikoshiiD-fructose 6-phosphate45.50.01522.99 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant A71EPyrococcus horikoshiiadenosine 5'-diphosphate—0.01362.82 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant A71EPyrococcus horikoshiiD-fructose 6-phosphate39.3—1.72 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant A71EPyrococcus horikoshiiD-glucose2.683.950.678 calc.50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant D17APyrococcus horikoshiiD-fructose 6-phosphate1.9822.60.087650 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant D433APyrococcus horikoshiiadenosine 5'-diphosphate—0.01390.34550 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant D433APyrococcus horikoshiiD-fructose 6-phosphate0.00480.01270.37850 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant K158APyrococcus horikoshiiadenosine 5'-diphosphate—0.03791.08 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant K158APyrococcus horikoshiiD-fructose 6-phosphate416.56.3150 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant N15APyrococcus horikoshiiadenosine 5'-diphosphate—0.02383.83 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant N15APyrococcus horikoshiiD-fructose 6-phosphate91.10.10388450 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant N160APyrococcus horikoshiiadenosine 5'-diphosphate—0.07242.09 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant N160APyrococcus horikoshiiD-fructose 6-phosphate1510.41536450 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant Q224APyrococcus horikoshiiadenosine 5'-diphosphate—0.02952.21 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant Q224APyrococcus horikoshiiD-fructose 6-phosphate65.30.03861.69 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant Q97APyrococcus horikoshiiadenosine 5'-diphosphate—0.007471.74 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant Q97APyrococcus horikoshiiD-fructose 6-phosphate130.007081.84 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant R185APyrococcus horikoshiiadenosine 5'-diphosphate—0.4730.74250 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant R185APyrococcus horikoshiiD-fructose 6-phosphate0.3510.3181.150 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant R191APyrococcus horikoshiiadenosine 5'-diphosphate—0.01451.89 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant R191APyrococcus horikoshiiD-fructose 6-phosphate27.40.254410750 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant R191EPyrococcus horikoshiiadenosine 5'-diphosphate—0.060570250 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant R191EPyrococcus horikoshiiD-fructose 6-phosphate42.54.878.7350 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant S183EPyrococcus horikoshiiadenosine 5'-diphosphate—0.02963.1 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant S183EPyrococcus horikoshiiD-fructose 6-phosphate91.70.08651.06 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant S189APyrococcus horikoshiiadenosine 5'-diphosphate—0.0161.6 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant S189APyrococcus horikoshiiD-fructose 6-phosphate25.50.009022.83 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant S263APyrococcus horikoshiiadenosine 5'-diphosphate—0.02083.08 × 10³50 °CPMID 19553681Open
ADP-dependent 6-phosphofructokinase (PhPFK)variant S263APyrococcus horikoshiiD-fructose 6-phosphate64.10.03951.62 × 10³50 °CPMID 19553681Open
ADP-dependent phosphofructokinase (phPFK)Pyrococcus horikoshiicobalt(II)-ADP complex980.0224.4 × 10³pH 6.5, 50 °CPMID 21906652Open
ADP-dependent phosphofructokinase (PhPFK)Pyrococcus horikoshiiD-fructose 6-phosphate210.056390pH 6.5, 40 °CPMID 28726643Open
ADP-dependent phosphofructokinase (phPFK)Pyrococcus horikoshiimagnesium-ADP complexMgADP varied; free Mg2+ held at 1 mM520.0086.5 × 10³pH 6.5, 50 °CPMID 21906652Open
ADP-dependent phosphofructokinase (phPFK)Pyrococcus horikoshiimagnesium-ADP complexMgADP varied at several free ADP concentrations710.00581.2 × 10⁴ calc.pH 6.5, 50 °CPMID 21906652Open
ADP-dependent phosphofructokinase (phPFK)Pyrococcus horikoshiimanganese(II)-ADP complex670.0088.4 × 10³pH 6.5, 50 °CPMID 21906652Open
ADP-dependent phosphofructokinase/glucokinase (MbPFK)Methanococcoides burtoniiadenosine 5'-diphosphate140.241pH 6.5, 25 °CPMID 28919057Open
ADP-dependent phosphofructokinase/glucokinase (MbPFK)Methanococcoides burtoniiD-fructose 6-phosphate8.90.023340pH 6.5, 25 °CPMID 28919057Open
ADP-dependent phosphofructokinase/glucokinase (MePFK)Methanohalobium evestigatumadenosine 5'-diphosphate160.2563pH 6.5, 40 °CPMID 28919057Open
ADP-dependent phosphofructokinase/glucokinase (MePFK)Methanohalobium evestigatumD-fructose 6-phosphate9.50.034280pH 6.5, 40 °CPMID 28919057Open
ADP-dependent phosphofructokinase/glucokinase (MmazPFK/GK)Methanosarcina mazeiD-fructose 6-phosphate4.60.0066694pH 6.5, 40 °CPMID 29997580Open
ADP-dependent phosphofructokinase/glucokinase (MmazPFK/GK)Methanosarcina mazeiD-glucose1.4120.119pH 7.8, 40 °CPMID 29997580Open
ADP-dependent phosphofructokinase/glucokinase (MmazPFK/GK)Methanosarcina mazeiMg2+-ADP6.10.07284.5pH 6.5, 40 °CPMID 29997580Open
ADP-dependent phosphofructokinase/glucokinase (MmazPFK/GK)Methanosarcina mazeiMg2+-ADP1.70.60.29pH 7.8, 40 °CPMID 29997580Open
ADP-dependent phosphofructokinase/glucokinase, last common ancestor of Methanosarcinales (AncMsPFK/GK)variant ancestral reconstructed sequence (AncMsPFK/GK)—D-fructose 6-phosphate60.006921pH 6.5, 40 °CPMID 29997580Open
ADP-dependent phosphofructokinase/glucokinase, last common ancestor of Methanosarcinales (AncMsPFK/GK)variant ancestral reconstructed sequence (AncMsPFK/GK)—Mg2+-ADP8.70.712.2pH 6.5, 40 °CPMID 29997580Open
bifunctional ADP-dependent phosphofructokinase/glucokinase (MjPFK/GK)Methanococcus jannaschiiD-fructose 6-phosphate2.70.0063420pH 6.5, 40 °CPMID 28726643Open
bifunctional ADP-dependent phosphofructokinase/glucokinase (MjPFK/GK)Methanococcus jannaschiiD-glucose51.14.5pH 7.8, 40 °CPMID 28726643Open
bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK)Methanococcus maripaludisfructose 1,6-bisphosphate0.450.123.75pH 7, 30 °CPMID 24860874Open
bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK)Methanococcus maripaludisfructose 6-phosphate16.50.065253pH 6.5, 30 °CPMID 24860874Open
bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK)Methanococcus maripaludisMg2+-ADPMgCl2 5 mM in excess over ADP (approx. 5 mM free Mg2+)3083.75pH 6.5, 30 °CPMID 24860874Open
bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK)Methanococcus maripaludisMg2+-ADPapprox. 1 mM free Mg2+ (Kd 676 µM for Mg-ADP used)—1.2—pH 6.5, 30 °CPMID 24860874Open
reconstructed ancestral ADP-dependent kinase ancGK/PFK—D-fructose 6-phosphate0.330.1192.8pH 6.5, 40 °CPMID 28726643Open
reconstructed ancestral ADP-dependent kinase ancGK/PFK—D-glucose0.2440.06pH 7.8, 40 °CPMID 28726643Open
reconstructed ancestral ADP-dependent kinase ancMT—D-fructose 6-phosphate12.90.35pH 6.5, 40 °CPMID 28726643Open
reconstructed ancestral ADP-dependent kinase ancMT—D-glucose4.1310.13pH 7.8, 40 °CPMID 28726643Open
reconstructed ancestral ADP-dependent kinase ancMTvariant E72A—D-fructose 6-phosphate0.382.80.14pH 6.5, 40 °CPMID 28726643Open
reconstructed ancestral ADP-dependent kinase ancMTvariant E72A—D-glucose0.3—8.1 × 10⁻⁵pH 7.8, 40 °CPMID 28726643Open