Values CatLog read from this paper
Bifunctional ADP-dependent phosphofructokinase/glucokinase activity in the order Methanococcales--biochemical characterization of the mesophilic enzyme from Methanococcus maripaludis
PMID 24860874doi:10.1111/febs.12757Open in Browse
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CatLog read 8 records (20 values) from this paper: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) from Methanococcus maripaludis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) | Methanococcus maripaludis | D-glucose | 23 | 40 | 0.575 | pH 7.8, 30 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK), D-glucose |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) | Methanococcus maripaludis | fructose 1,6-bisphosphate | 0.45 | 0.12 | 3.75 | pH 7, 30 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK), fructose 1,6-bisphosphate |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) | Methanococcus maripaludis | fructose 6-phosphate | 16.5 | 0.065 | 253 | pH 6.5, 30 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK), fructose 6-phosphate |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) | Methanococcus maripaludis | glucose 6-phosphate | 0.678 | 0.623 | 1.09 | pH 7, 30 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK), glucose 6-phosphate |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) | Methanococcus maripaludis | Mg2+-ADPMgCl2 5 mM in excess over ADP (approx. 5 mM free Mg2+) | 48 | 16 | 3 | pH 7.8, 30 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK), Mg2+-ADP |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) | Methanococcus maripaludis | Mg2+-ADPMgCl2 5 mM in excess over ADP (approx. 5 mM free Mg2+) | 30 | 8 | 3.75 | pH 6.5, 30 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK), Mg2+-ADP |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) | Methanococcus maripaludis | Mg2+-ADPapprox. 1 mM free Mg2+ (Kd 676 µM for Mg-ADP used) | — | 0.3 | — | pH 7.8, 30 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK), Mg2+-ADP |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK) | Methanococcus maripaludis | Mg2+-ADPapprox. 1 mM free Mg2+ (Kd 676 µM for Mg-ADP used) | — | 1.2 | — | pH 6.5, 30 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MmPFK/GK), Mg2+-ADP |