Values CatLog read from this paper
Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases
PMID 28726643doi:10.1074/jbc.m117.790865Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (46 values) from this paper: reconstructed ancestral ADP-dependent kinase ancMT, bifunctional ADP-dependent phosphofructokinase/glucokinase (MjPFK/GK), reconstructed ancestral ADP-dependent kinase ancGK/PFK and 5 more from Methanococcus jannaschii, Thermococcus litoralis, Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ADP-dependent glucokinase (TlGK) | Thermococcus litoralis | D-glucose | 42 | 0.44 | 95 | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (TlGK), D-glucose |
| ADP-dependent phosphofructokinase (PhPFK) | Pyrococcus horikoshii | D-fructose 6-phosphate | 21 | 0.056 | 390 | pH 6.5, 40 °C | Open record: ADP-dependent phosphofructokinase (PhPFK), D-fructose 6-phosphate |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MjPFK/GK) | Methanococcus jannaschii | D-fructose 6-phosphate | 2.7 | 0.0063 | 420 | pH 6.5, 40 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MjPFK/GK), D-fructose 6-phosphate |
| bifunctional ADP-dependent phosphofructokinase/glucokinase (MjPFK/GK) | Methanococcus jannaschii | D-glucose | 5 | 1.1 | 4.5 | pH 7.8, 40 °C | Open record: bifunctional ADP-dependent phosphofructokinase/glucokinase (MjPFK/GK), D-glucose |
| reconstructed ancestral ADP-dependent kinase ancGK/PFK | — | D-fructose 6-phosphate | 0.33 | 0.119 | 2.8 | pH 6.5, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancGK/PFK, D-fructose 6-phosphate |
| reconstructed ancestral ADP-dependent kinase ancGK/PFK | — | D-glucose | 0.24 | 4 | 0.06 | pH 7.8, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancGK/PFK, D-glucose |
| reconstructed ancestral ADP-dependent kinase ancM | — | D-fructose 6-phosphate | 8.5 | 0.37 | 23 | pH 6.5, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancM, D-fructose 6-phosphate |
| reconstructed ancestral ADP-dependent kinase ancM | — | D-glucose | 15 | 5.7 | 2.6 | pH 7.8, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancM, D-glucose |
| reconstructed ancestral ADP-dependent kinase ancMMT | — | D-fructose 6-phosphate | 0.53 | 0.54 | 0.97 | pH 6.5, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancMMT, D-fructose 6-phosphate |
| reconstructed ancestral ADP-dependent kinase ancMMT | — | D-glucose | 0.35 | 12 | 0.033 | pH 7.8, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancMMT, D-glucose |
| reconstructed ancestral ADP-dependent kinase ancMT | — | D-fructose 6-phosphate | 1 | 2.9 | 0.35 | pH 6.5, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancMT, D-fructose 6-phosphate |
| reconstructed ancestral ADP-dependent kinase ancMT | — | D-glucose | 4.1 | 31 | 0.13 | pH 7.8, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancMT, D-glucose |
| reconstructed ancestral ADP-dependent kinase ancMTvariant E72A | — | D-fructose 6-phosphate | 0.38 | 2.8 | 0.14 | pH 6.5, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancMT variant E72A, D-fructose 6-phosphate |
| reconstructed ancestral ADP-dependent kinase ancMTvariant E72A | — | D-glucose | 0.3 | — | 8.1 × 10⁻⁵ | pH 7.8, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancMT variant E72A, D-glucose |
| reconstructed ancestral ADP-dependent kinase ancT | — | D-fructose 6-phosphate | 43 | 0.06 | 720 | pH 6.5, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancT, D-fructose 6-phosphate |
| reconstructed ancestral ADP-dependent kinase ancT | — | D-glucose | 15 | — | 0.011 | pH 7.8, 40 °C | Open record: reconstructed ancestral ADP-dependent kinase ancT, D-glucose |