Values CatLog read from this paper
Catalytic and regulatory roles of divalent metal cations on the phosphoryl-transfer mechanism of ADP-dependent sugar kinases from hyperthermophilic archaea
PMID 21906652doi:10.1016/j.biochi.2011.08.021Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (37 values) from this paper: ADP-dependent glucokinase (pfGK), ADP-dependent glucokinase (tlGK), ADP-dependent phosphofructokinase (phPFK) from Pyrococcus furiosus, Thermococcus litoralis, Pyrococcus horikoshii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 5 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ADP-dependent glucokinase (pfGK) | Pyrococcus furiosus | cobalt(II)-ADP complex | 114 | 0.025 | 4.6 × 10³ | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (pfGK), cobalt(II)-ADP complex |
| ADP-dependent glucokinase (pfGK) | Pyrococcus furiosus | magnesium-ADP complexMgADP varied; free Mg2+ held at 1 mM | 104 | 0.014 | 7.4 × 10³ | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (pfGK), magnesium-ADP complex |
| ADP-dependent glucokinase (pfGK) | Pyrococcus furiosus | magnesium-ADP complexMgADP varied at several free ADP concentrations | 139 | 0.0046 | 3.0 × 10⁴ calc. | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (pfGK), magnesium-ADP complex |
| ADP-dependent glucokinase (pfGK) | Pyrococcus furiosus | manganese(II)-ADP complexMnADP varied; free Mn2+ held at 1 mM | 116 | 0.022 | 5.3 × 10³ | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (pfGK), manganese(II)-ADP complex |
| ADP-dependent glucokinase (pfGK) | Pyrococcus furiosus | manganese(II)-ADP complexMnADP varied at several free Mn2+ concentrations | 255 | 0.028 | 9.1 × 10³ calc. | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (pfGK), manganese(II)-ADP complex |
| ADP-dependent glucokinase (tlGK) | Thermococcus litoralis | cobalt(II)-ADP complex | 44 | 0.015 | 2.9 × 10³ | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (tlGK), cobalt(II)-ADP complex |
| ADP-dependent glucokinase (tlGK) | Thermococcus litoralis | magnesium-ADP complexMgADP varied; free Mg2+ held at 1 mM | 35 | 0.023 | 1.5 × 10³ | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (tlGK), magnesium-ADP complex |
| ADP-dependent glucokinase (tlGK) | Thermococcus litoralis | magnesium-ADP complexMgADP varied at several free ADP concentrations | 51 | 0.0021 | 2.4 × 10⁴ calc. | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (tlGK), magnesium-ADP complex |
| ADP-dependent glucokinase (tlGK) | Thermococcus litoralis | manganese(II)-ADP complexMnADP varied; free Mn2+ held at 1 mM | 21 | 0.017 | 1.2 × 10³ | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (tlGK), manganese(II)-ADP complex |
| ADP-dependent glucokinase (tlGK) | Thermococcus litoralis | manganese(II)-ADP complexMnADP varied at several free Mn2+ concentrations | 25 | 0.011 | 2.3 × 10³ calc. | pH 7.8, 40 °C | Open record: ADP-dependent glucokinase (tlGK), manganese(II)-ADP complex |
| ADP-dependent phosphofructokinase (phPFK) | Pyrococcus horikoshii | cobalt(II)-ADP complex | 98 | 0.022 | 4.4 × 10³ | pH 6.5, 50 °C | Open record: ADP-dependent phosphofructokinase (phPFK), cobalt(II)-ADP complex |
| ADP-dependent phosphofructokinase (phPFK) | Pyrococcus horikoshii | magnesium-ADP complexMgADP varied; free Mg2+ held at 1 mM | 52 | 0.008 | 6.5 × 10³ | pH 6.5, 50 °C | Open record: ADP-dependent phosphofructokinase (phPFK), magnesium-ADP complex |
| ADP-dependent phosphofructokinase (phPFK) | Pyrococcus horikoshii | magnesium-ADP complexMgADP varied at several free ADP concentrations | 71 | 0.0058 | 1.2 × 10⁴ calc. | pH 6.5, 50 °C | Open record: ADP-dependent phosphofructokinase (phPFK), magnesium-ADP complex |
| ADP-dependent phosphofructokinase (phPFK) | Pyrococcus horikoshii | manganese(II)-ADP complex | 67 | 0.008 | 8.4 × 10³ | pH 6.5, 50 °C | Open record: ADP-dependent phosphofructokinase (phPFK), manganese(II)-ADP complex |