Values CatLog read from this paper
Refolding, characterization and crystal structure of (S)-malate dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix
PMID 19555779doi:10.1016/j.bbapap.2009.06.014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (43 values) from this paper: ApeMDH ((S)-malate dehydrogenase), MjMDH ((S)-malate dehydrogenase), AfMDH ((S)-malate dehydrogenase) from Aeropyrum pernix, Methanocaldococcus jannaschii, Archaeoglobus fulgidus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| AfMDH ((S)-malate dehydrogenase) | Archaeoglobus fulgidus | (2S,3R)-tartrate | 0.33 | 0.4 | 0.83 | pH 10, 50 °C | Open record: AfMDH ((S)-malate dehydrogenase), (2S,3R)-tartrate |
| AfMDH ((S)-malate dehydrogenase) | Archaeoglobus fulgidus | (2S,3S)-tartrate | 1.2 | 0.63 | 2.3 | pH 10, 50 °C | Open record: AfMDH ((S)-malate dehydrogenase), (2S,3S)-tartrate |
| AfMDH ((S)-malate dehydrogenase) | Archaeoglobus fulgidus | (S)-malate | 5.7 | 0.074 | 77 | pH 10, 50 °C | Open record: AfMDH ((S)-malate dehydrogenase), (S)-malate |
| ApeMDH ((S)-malate dehydrogenase) | Aeropyrum pernix | (2S,3R)-tartrate4 mM NAD (Methods); cosubstrate NAD | 0.16 | 0.2 | 0.8 | pH 10, 50 °C | Open record: ApeMDH ((S)-malate dehydrogenase), (2S,3R)-tartrate |
| ApeMDH ((S)-malate dehydrogenase) | Aeropyrum pernix | (2S,3R)-tartrate1 mM NADP; cosubstrate NADP | 0.2 | 5.8 | 0.034 | pH 10, 50 °C | Open record: ApeMDH ((S)-malate dehydrogenase), (2S,3R)-tartrate |
| ApeMDH ((S)-malate dehydrogenase) | Aeropyrum pernix | (2S,3S)-tartrate4 mM NAD (Methods); cosubstrate NAD | 0.37 | 1.2 | 0.31 | pH 10, 50 °C | Open record: ApeMDH ((S)-malate dehydrogenase), (2S,3S)-tartrate |
| ApeMDH ((S)-malate dehydrogenase) | Aeropyrum pernix | (2S,3S)-tartrate1 mM NADP; cosubstrate NADP | 4.1 | 23 | 0.18 | pH 10, 50 °C | Open record: ApeMDH ((S)-malate dehydrogenase), (2S,3S)-tartrate |
| ApeMDH ((S)-malate dehydrogenase) | Aeropyrum pernix | (S)-malate4 mM NAD (Methods); cosubstrate NAD | 2.6 | 0.12 | 22 | pH 10, 50 °C | Open record: ApeMDH ((S)-malate dehydrogenase), (S)-malate |
| ApeMDH ((S)-malate dehydrogenase) | Aeropyrum pernix | (S)-malate1 mM NADP; cosubstrate NADP | 1.4 | 0.019 | 74 | pH 10, 50 °C | Open record: ApeMDH ((S)-malate dehydrogenase), (S)-malate |
| ApeMDH ((S)-malate dehydrogenase) | Aeropyrum pernix | NAD | 2.8 | 0.87 | 3.2 calc. | pH 10, 50 °C | Open record: ApeMDH ((S)-malate dehydrogenase), NAD |
| ApeMDH ((S)-malate dehydrogenase) | Aeropyrum pernix | NADP | 1.6 | 0.0076 | 210 calc. | pH 10, 50 °C | Open record: ApeMDH ((S)-malate dehydrogenase), NADP |
| MjMDH ((S)-malate dehydrogenase) | Methanocaldococcus jannaschii | (2S,3S)-tartrate1 mM NAD; cosubstrate NAD | 0.2 | 11 | 0.018 | pH 10, 50 °C | Open record: MjMDH ((S)-malate dehydrogenase), (2S,3S)-tartrate |
| MjMDH ((S)-malate dehydrogenase) | Methanocaldococcus jannaschii | (2S,3S)-tartrate1 mM NADP; cosubstrate NADP | 0.15 | 5.1 | 0.029 | pH 10, 50 °C | Open record: MjMDH ((S)-malate dehydrogenase), (2S,3S)-tartrate |
| MjMDH ((S)-malate dehydrogenase) | Methanocaldococcus jannaschii | (S)-malate1 mM NAD; cosubstrate NAD | 0.33 | 0.41 | 0.8 | pH 10, 50 °C | Open record: MjMDH ((S)-malate dehydrogenase), (S)-malate |
| MjMDH ((S)-malate dehydrogenase) | Methanocaldococcus jannaschii | (S)-malate1 mM NADP; cosubstrate NADP | 0.069 | 0.084 | 0.82 | pH 10, 50 °C | Open record: MjMDH ((S)-malate dehydrogenase), (S)-malate |