Values CatLog read from this paper
The flexible motif V of Epstein-Barr virus deoxyuridine 5'-triphosphate pyrophosphatase is essential for catalysis
PMID 19586911doi:10.1074/jbc.m109.019315Open in Browse
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CatLog read 15 records (25 values) from this paper: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) from Epstein-Barr virus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM | — | 8 × 10⁻⁴ | — | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM | 1.4 | — | — | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM | — | — | 1.8 × 10³ | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphateno MgCl2 added; Mg2+ < 0.08 µM by atomic absorption | — | 0.006 | — | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphateno MgCl2 added; Mg2+ < 0.08 µM by atomic absorption | 1.2 | — | — | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphateno MgCl2 added; Mg2+ < 0.08 µM by atomic absorption | — | — | 200 | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM | — | 7.1 × 10⁻⁴ | — | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM | 1.6 | — | — | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM | — | — | 2.3 × 10³ | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate | 1.2 | — | — | pH 7.5, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant C4S | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate | 1.7 | 6 × 10⁻⁴ | 2.8 × 10³ | pH 7.8, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) variant C4S, 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant D131N | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate | 0.04 | 0.007 | 6 | pH 7.5, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) variant D131N, 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant D131S | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate | 0.04 | 0.0052 | 8 | pH 7.5, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) variant D131S, 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant D131S/G78D | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate | 0.18 | 0.009 | 20 | pH 7.5, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) variant D131S/G78D, 2'-deoxyuridine 5'-triphosphate |
| deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant R268A | Epstein-Barr virus | 2'-deoxyuridine 5'-triphosphate | 0.06 | 0.008 | 8 | pH 7.5, 25 °C | Open record: deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase) variant R268A, 2'-deoxyuridine 5'-triphosphate |