CatLogenzyme kinetics catalog

EC 3.6.1.23: dUTP diphosphatase

HydrolasesOpen in Browse

CatLog read 89 records for EC 3.6.1.23 from 8 papers, for 12 organisms. Names in these papers: dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112), deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase), dUTPase (PfdUTPase), dUTPase (ASFV dUTPase, aDUT) and 8 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 75 records for EC 3.6.1.23 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.6.1.23 in Browse.

Papers

PaperOrganismsRecords
The dUTPase of white spot syndrome virus assembles its active sites in a noncanonical mannerEscherichia coli, White spot syndrome virus21
Kinetic properties and specificity of trimeric Plasmodium falciparum and human dUTPasesHomo sapiens, Plasmodium falciparum17
The flexible motif V of Epstein-Barr virus deoxyuridine 5'-triphosphate pyrophosphatase is essential for catalysisEpstein-Barr virus15
Structural comparisons of host and African swine fever virus dUTPases reveal new clues for inhibitor developmentAfrican swine fever virus, Sus scrofa10
Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Leishmania majorLeishmania major8
Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzymeSaccharomyces cerevisiae6
Enzyme kinetics of dUTPase from the planarian Dugesia ryukyuensisDugesia ryukyuensis6
Functional convergence in Z-DNA biosynthesis highlighted by the characterization of nucleotide metabolism enzymes in bacteriophagesArthrobacter phage Wayne, Gordonia phage Ghobes6

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM—8 × 10⁻⁴—pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM1.4——pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM——1.8 × 10³pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphateno MgCl2 added; Mg2+ < 0.08 µM by atomic absorption—0.006—pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphateno MgCl2 added; Mg2+ < 0.08 µM by atomic absorption1.2——pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphateno MgCl2 added; Mg2+ < 0.08 µM by atomic absorption——200pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM—7.1 × 10⁻⁴—pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM1.6——pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphate25 µM cresol red; 150 mM KCl; 1 mM MgCl2; enzyme 31 nM——2.3 × 10³pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)Epstein-Barr virus2'-deoxyuridine 5'-triphosphate1.2——pH 7.5, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant C4SEpstein-Barr virus2'-deoxyuridine 5'-triphosphate1.76 × 10⁻⁴2.8 × 10³pH 7.8, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant D131NEpstein-Barr virus2'-deoxyuridine 5'-triphosphate0.040.0076pH 7.5, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant D131SEpstein-Barr virus2'-deoxyuridine 5'-triphosphate0.040.00528pH 7.5, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant D131S/G78DEpstein-Barr virus2'-deoxyuridine 5'-triphosphate0.180.00920pH 7.5, 25 °CPMID 19586911Open
deoxyuridine 5'-triphosphate pyrophosphatase (dUTPase)variant R268AEpstein-Barr virus2'-deoxyuridine 5'-triphosphate0.060.0088pH 7.5, 25 °CPMID 19586911Open
DmtZ-DutZArthrobacter phage Wayne2'-deoxyadenosine 5'-triphosphate5.87——pH 7.5PMID 41657244Open
DmtZ-DutZArthrobacter phage Wayne2'-deoxyguanosine 5'-triphosphate0.56——pH 7.5PMID 41657244Open
DmtZ-DutZArthrobacter phage Wayne2'-deoxyuridine 5'-triphosphate16.43——pH 7.5PMID 41657244Open
dUTP pyrophosphatase DUT1 (dUTPase)Saccharomyces cerevisiae2'-deoxyinosine 5'-triphosphate1.3——pH 7.5, 30 °CPMID 21548881Open
dUTP pyrophosphatase DUT1 (dUTPase)Saccharomyces cerevisiae2'-deoxyuridine 5'-triphosphate9.6——pH 7.5, 30 °CPMID 21548881Open
dUTP pyrophosphatase DUT1 (dUTPase)variant Q114ASaccharomyces cerevisiae2'-deoxyuridine 5'-triphosphate1.30.014290pH 7.5, 30 °CPMID 21548881Open
dUTP pyrophosphatase DUT1 (dUTPase)variant R111ASaccharomyces cerevisiae2'-deoxyuridine 5'-triphosphate3.3——pH 7.5, 30 °CPMID 21548881Open
dUTP pyrophosphatase DUT1 (dUTPase)variant Y88ASaccharomyces cerevisiae2'-deoxyuridine 5'-triphosphate7.4——pH 7.5, 30 °CPMID 21548881Open
dUTP pyrophosphatase DUT1 (dUTPase)variant Y88ASaccharomyces cerevisiaeuridine 5'-triphosphate5.3——pH 7.5, 30 °CPMID 21548881Open
dUTPase (ASFV dUTPase, aDUT)African swine fever virus2'-deoxyuridine 5'-triphosphate4.379.8 × 10⁻⁴4.46 × 10³pH 7.5, 25 °CPMID 33139328Open
dUTPase (ASFV dUTPase, aDUT)variant D29AAfrican swine fever virus2'-deoxyuridine 5'-triphosphate0.310.0068946.2pH 7.5, 25 °CPMID 33139328Open
dUTPase (ASFV dUTPase, aDUT)variant F154YAfrican swine fever virus2'-deoxyuridine 5'-triphosphate1.50.0264556pH 7.5, 25 °CPMID 33139328Open
dUTPase (ASFV dUTPase, aDUT)variant I90AAfrican swine fever virus2'-deoxyuridine 5'-triphosphate0.754.7 × 10⁻⁴1.58 × 10³pH 7.5, 25 °CPMID 33139328Open
dUTPase (ASFV dUTPase, aDUT)variant L89AAfrican swine fever virus2'-deoxyuridine 5'-triphosphate2.940.00544540pH 7.5, 25 °CPMID 33139328Open
dUTPase (ASFV dUTPase, aDUT)variant Q120AAfrican swine fever virus2'-deoxyuridine 5'-triphosphate0.580.0167635.1pH 7.5, 25 °CPMID 33139328Open
dUTPase (ASFV dUTPase, aDUT)variant R149KAfrican swine fever virus2'-deoxyuridine 5'-triphosphate0.150.0045133pH 7.5, 25 °CPMID 33139328Open
dUTPase (ASFV dUTPase, aDUT)variant S72AAfrican swine fever virus2'-deoxyuridine 5'-triphosphate0.480.0048998.5pH 7.5, 25 °CPMID 33139328Open
dUTPase (ASFV dUTPase, aDUT)variant S73AAfrican swine fever virus2'-deoxyuridine 5'-triphosphate5.580.004161.34 × 10³pH 7.5, 25 °CPMID 33139328Open
dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66KDugesia ryukyuensis2'-deoxyadenosine 5'-triphosphate1.50.09416pH 8.2, 25 °CPMID 30902068Open
dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66KDugesia ryukyuensis2'-deoxycytidine 5'-triphosphate1.60.07721pH 8.2, 25 °CPMID 30902068Open
dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66KDugesia ryukyuensis2'-deoxyguanosine 5'-triphosphate2.90.2114pH 8.2, 25 °CPMID 30902068Open
dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66KDugesia ryukyuensis2'-deoxyuridine 5'-triphosphate5 mM MgCl2; 10 µM dUTP; 0.2 µM enzyme; n = 7340.0048.5 × 10³pH 8.2, 25 °CPMID 30902068Open
dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66KDugesia ryukyuensis2'-deoxyuridine 5'-triphosphate5 mM MnCl2 in place of MgCl2; n = 3480.00736.5 × 10³pH 8.2, 25 °CPMID 30902068Open
dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66KDugesia ryukyuensisthymidine 5'-triphosphate1.40.1113pH 8.2, 25 °CPMID 30902068Open
dUTPase (dUTP nucleotidohydrolase; dUTPase-dUDPase)Leishmania major2'-deoxyadenosine 5'-triphosphate5.41.0345.1pH 8, 25 °CPMID 11420444Open
dUTPase (dUTP nucleotidohydrolase; dUTPase-dUDPase)Leishmania major2'-deoxycytidine 5'-triphosphate13—5pH 8, 25 °CPMID 11420444Open
dUTPase (dUTP nucleotidohydrolase; dUTPase-dUDPase)Leishmania major2'-deoxyguanosine 5'-triphosphate6.4—6.4pH 8, 25 °CPMID 11420444Open
dUTPase (dUTP nucleotidohydrolase; dUTPase-dUDPase)Leishmania major2'-deoxythymidine 5'-triphosphate8.61.5145.5pH 8, 25 °CPMID 11420444Open
dUTPase (dUTP nucleotidohydrolase; dUTPase-dUDPase)Leishmania major2'-deoxyuridine 5'-diphosphate650.06271 × 10³pH 8, 25 °CPMID 11420444Open
dUTPase (dUTP nucleotidohydrolase; dUTPase-dUDPase)Leishmania major2'-deoxyuridine 5'-triphosphatestopped-flow pH-indicator assay; 1 mg/ml BSA—0.002112.3 × 10⁴pH 8, 25 °CPMID 11420444Open
dUTPase (dUTP nucleotidohydrolase; dUTPase-dUDPase)Leishmania major2'-deoxyuridine 5'-triphosphate0.5 mM cresol red; 25 mM BICINE49——pH 8, 25 °CPMID 11420444Open
dUTPase (dUTP nucleotidohydrolase; dUTPase-dUDPase)Leishmania major2'-deoxyuridine 5'-triphosphate—0.00235—25 °CPMID 11420444Open
dUTPase (E. coli dUTPase, eDUT)Escherichia coli2'-deoxyuridine 5'-triphosphate9.39.3 × 10⁻⁴1 × 10⁴pH 7.5, 20 °CPMID 29187596Open
dUTPase (E. coli dUTPase, eDUT)variant R116SEscherichia coli2'-deoxyuridine 5'-triphosphate2.70.0052520pH 7.5, 20 °CPMID 29187596Open
dUTPase (E. coli dUTPase, eDUT)variant T22R/R116SEscherichia coli2'-deoxyuridine 5'-triphosphate3.30.013250pH 7.5, 20 °CPMID 29187596Open
dUTPase (hdUTPase)Homo sapiens2'-deoxycytidine 5'-triphosphate—0.6870.1616pH 7, 25 °CPMID 19879316Open
dUTPase (hdUTPase)Homo sapiens2'-deoxyuridine 5'-triphosphate—0.00116.6 × 10³pH 7, 25 °CPMID 19879316Open
dUTPase (hdUTPase)Homo sapiens5-fluoro-2'-deoxyuridine 5'-triphosphate—0.0061600pH 7, 25 °CPMID 19879316Open
dUTPase (hdUTPase)Homo sapiens5-fluoro-2'-deoxyuridine 5'-triphosphate3.20.0042760 calc.25 °CPMID 19879316Open
dUTPase (hdUTPase)Homo sapiens5-iodo-2'-deoxyuridine 5'-triphosphate—0.5230.0061pH 7, 25 °CPMID 19879316Open
dUTPase (hdUTPase)Homo sapiensthymidine 5'-triphosphate—0.7610.01971pH 7, 25 °CPMID 19879316Open
dUTPase (hdUTPase)Homo sapiensuridine 5'-triphosphate—0.540.01278pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparum2'-deoxyadenosine 5'-triphosphate—1.130.00265pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparum2'-deoxycytidine 5'-triphosphate—0.4650.02172pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparum2'-deoxyguanosine 5'-triphosphate—1.961.3 × 10⁻⁴pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparum2'-deoxyuridine 5'-triphosphate—0.001877.1 × 10³pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparum5-fluoro-2'-deoxyuridine 5'-triphosphate—0.00173110pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparum5-fluoro-2'-deoxyuridine 5'-triphosphate40.00311.3 × 10³ calc.25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparum5-iodo-2'-deoxyuridine 5'-triphosphate—0.4380.00525pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparum5-iodo-2'-deoxyuridine 5'-triphosphatekcat per active site0.002——pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparumthymidine 5'-triphosphate—0.290.0092pH 7, 25 °CPMID 19879316Open
dUTPase (PfdUTPase)Plasmodium falciparumuridine 5'-triphosphate—0.5710.01121pH 7, 25 °CPMID 19879316Open
dUTPase (swine dUTPase, sDUT)Sus scrofa2'-deoxyuridine 5'-triphosphate5.585.8 × 10⁻⁴9.6 × 10³pH 7.5, 25 °CPMID 33139328Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)White spot syndrome virus2'-deoxyuridine 5'-triphosphate7.40.00174.4 × 10³pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant D170GWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate6.20.00213 × 10³pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant D170LWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate0.530.007175pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant D34NWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate0.920.01371pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant F166YWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate1.20.005240pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant K150AWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate5.90.00252.4 × 10³pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant K150EWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate5.50.00262.1 × 10³pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant R158AWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate3.20.00261.2 × 10³pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant R158EWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate2.60.00261 × 10³pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant R158WWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate3.10.0032970pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant R161KWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate0.0530.00549.8pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant R24TWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate0.450.0029160pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant R24T/S115RWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate0.510.006776pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant R24T/S115R/D170GWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate3.10.0042740pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant S115RWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate2.60.0045580pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant V160DWhite spot syndrome virus2'-deoxyuridine 5'-triphosphate7.40.0023.7 × 10³pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant Δ134-153White spot syndrome virus2'-deoxyuridine 5'-triphosphate0.670.02132pH 7.5, 20 °CPMID 29187596Open
dUTPase (WSSV dUTPase, wDUT; N-terminal dUTPase domain of WSV112)variant Δ170-171White spot syndrome virus2'-deoxyuridine 5'-triphosphate1.70.0028610pH 7.5, 20 °CPMID 29187596Open
DutZGordonia phage Ghobes2'-deoxyadenosine 5'-triphosphate12.86——pH 7.5PMID 41657244Open
DutZGordonia phage Ghobes2'-deoxyguanosine 5'-triphosphate1.36——pH 7.5PMID 41657244Open
DutZGordonia phage Ghobes2'-deoxyuridine 5'-triphosphate4.48——pH 7.5PMID 41657244Open