Values CatLog read from this paper
Enzyme kinetics of dUTPase from the planarian Dugesia ryukyuensis
PMID 30902068doi:10.1186/s13104-019-4191-6Open in Browse
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CatLog read 6 records (18 values) from this paper: dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase) from Dugesia ryukyuensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66K | Dugesia ryukyuensis | 2'-deoxyadenosine 5'-triphosphate | 1.5 | 0.094 | 16 | pH 8.2, 25 °C | Open record: dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase) variant R66K, 2'-deoxyadenosine 5'-triphosphate |
| dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66K | Dugesia ryukyuensis | 2'-deoxycytidine 5'-triphosphate | 1.6 | 0.077 | 21 | pH 8.2, 25 °C | Open record: dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase) variant R66K, 2'-deoxycytidine 5'-triphosphate |
| dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66K | Dugesia ryukyuensis | 2'-deoxyguanosine 5'-triphosphate | 2.9 | 0.21 | 14 | pH 8.2, 25 °C | Open record: dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase) variant R66K, 2'-deoxyguanosine 5'-triphosphate |
| dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66K | Dugesia ryukyuensis | 2'-deoxyuridine 5'-triphosphate5 mM MgCl2; 10 µM dUTP; 0.2 µM enzyme; n = 7 | 34 | 0.004 | 8.5 × 10³ | pH 8.2, 25 °C | Open record: dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase) variant R66K, 2'-deoxyuridine 5'-triphosphate |
| dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66K | Dugesia ryukyuensis | 2'-deoxyuridine 5'-triphosphate5 mM MnCl2 in place of MgCl2; n = 3 | 48 | 0.0073 | 6.5 × 10³ | pH 8.2, 25 °C | Open record: dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase) variant R66K, 2'-deoxyuridine 5'-triphosphate |
| dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase)variant R66K | Dugesia ryukyuensis | thymidine 5'-triphosphate | 1.4 | 0.11 | 13 | pH 8.2, 25 °C | Open record: dUTPase (deoxyuridine 5'-triphosphate pyrophosphatase) variant R66K, thymidine 5'-triphosphate |