Values CatLog read from this paper
Mutagenesis studies toward understanding allostery in thrombin
PMID 19640005doi:10.1021/bi900921tOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (20 values) from this paper: thrombin.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 10 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| thrombin | — | H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl | 75 | 0.0059 | 1.3 × 10⁴ calc. | pH 7.5 | Open record: thrombin, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombin | — | H-D-Phe-Pip-Arg-p-nitroanilide0.2 M choline chloride (Na+-free) | 15.8 | 0.0088 | 1.8 × 10³ calc. | pH 7.5 | Open record: thrombin, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombin | — | H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl; 1 mM DTT | 46.6 | 0.0064 | 7.3 × 10³ calc. | pH 7.5 | Open record: thrombin, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombin | — | H-D-Phe-Pip-Arg-p-nitroanilide1.0 M NaCl; 1 mM DTT | 55.5 | 0.0039 | 1.4 × 10⁴ calc. | pH 7.5 | Open record: thrombin, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombinvariant E217C/K224C | — | H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl | 0.32 | 0.062 | 5.2 calc. | pH 7.5 | Open record: thrombin variant E217C/K224C, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombinvariant E217C/K224C | — | H-D-Phe-Pip-Arg-p-nitroanilide0.2 M choline chloride (Na+-free) | 0.11 | 0.298 | 0.37 calc. | pH 7.5 | Open record: thrombin variant E217C/K224C, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombinvariant E217C/K224C | — | H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl; 1 mM DTT | 41.2 | 0.0956 | 431 calc. | pH 7.5 | Open record: thrombin variant E217C/K224C, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombinvariant E217C/K224C | — | H-D-Phe-Pip-Arg-p-nitroanilide1.0 M NaCl; 1 mM DTT | 47.9 | 0.0287 | 1.67 × 10³ calc. | pH 7.5 | Open record: thrombin variant E217C/K224C, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombinvariant R67C/I82C | — | H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl | 84.4 | 0.0059 | 1.4 × 10⁴ calc. | pH 7.5 | Open record: thrombin variant R67C/I82C, H-D-Phe-Pip-Arg-p-nitroanilide |
| thrombinvariant R67C/I82C | — | H-D-Phe-Pip-Arg-p-nitroanilide0.2 M choline chloride (Na+-free) | 21.2 | 0.0106 | 2.00 × 10³ calc. | pH 7.5 | Open record: thrombin variant R67C/I82C, H-D-Phe-Pip-Arg-p-nitroanilide |