CatLogenzyme kinetics catalog

EC 3.4.21.5: thrombin

HydrolasesOpen in Browse

CatLog read 85 records for EC 3.4.21.5 from 7 papers, for 2 organisms. Names in these papers: thrombin, α-thrombin.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 13 records for EC 3.4.21.5 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.4.21.5 in Browse.

Papers

PaperOrganismsRecords
Mutant N143P reveals how Na+ activates thrombinHomo sapiens23
Roles of low specificity and cofactor interaction sites on thrombin during factor XIII activation. Competition for cofactor sites on thrombin determines its fateHomo sapiens20
Long range communication between exosites 1 and 2 modulates thrombin functionHomo sapiens12
Functional analysis of mutant variants of thrombin-activatable fibrinolysis inhibitor resistant to activation by thrombin or plasmin—10
Mutagenesis studies toward understanding allostery in thrombin—10
Modifications of the Prothrombin Active Site S4 Subpocket Confer Resistance to DabigatranHomo sapiens8
Purification and characterization of a coagulant enzyme, okinaxobin II, from Trimeresurus okinavensis (himehabu snake) venom which release fibrinopeptides A and BBos taurus2

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
thrombin—H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl750.00591.3 × 10⁴ calc.pH 7.5PMID 19640005Open
thrombin—H-D-Phe-Pip-Arg-p-nitroanilide0.2 M choline chloride (Na+-free)15.80.00881.8 × 10³ calc.pH 7.5PMID 19640005Open
thrombin—H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl; 1 mM DTT46.60.00647.3 × 10³ calc.pH 7.5PMID 19640005Open
thrombin—H-D-Phe-Pip-Arg-p-nitroanilide1.0 M NaCl; 1 mM DTT55.50.00391.4 × 10⁴ calc.pH 7.5PMID 19640005Open
thrombin—thrombin-activatable fibrinolysis inhibitor carrying the A93V substitutionno thrombomodulin——0.2PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor carrying the A93V substitution0.2051.69 × 10⁻⁴1.21 × 10³PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor carrying the P91S substitution——7.2PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor carrying the R92K substitution0.2152.29 × 10⁻⁴940PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor carrying the S90P substitution0.3771.62 × 10⁻⁴2.32 × 10³PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor carrying the S94V substitutionno thrombomodulin——1.1PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor carrying the S94V substitution0.3282.32 × 10⁻⁴1.41 × 10³PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor with aspartic acid at P3 and valines at P1' and P2' (DVV variant of Table 1)——15PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor, wild typeno thrombomodulin——0.4PMID 19335449Open
thrombin—thrombin-activatable fibrinolysis inhibitor, wild type0.3151.78 × 10⁻⁴1.77 × 10³PMID 19335449Open
thrombinvariant E217C/K224C—H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl0.320.0625.2 calc.pH 7.5PMID 19640005Open
thrombinvariant E217C/K224C—H-D-Phe-Pip-Arg-p-nitroanilide0.2 M choline chloride (Na+-free)0.110.2980.37 calc.pH 7.5PMID 19640005Open
thrombinvariant E217C/K224C—H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl; 1 mM DTT41.20.0956431 calc.pH 7.5PMID 19640005Open
thrombinvariant E217C/K224C—H-D-Phe-Pip-Arg-p-nitroanilide1.0 M NaCl; 1 mM DTT47.90.02871.67 × 10³ calc.pH 7.5PMID 19640005Open
thrombinvariant R67C/I82C—H-D-Phe-Pip-Arg-p-nitroanilide0.2 M NaCl84.40.00591.4 × 10⁴ calc.pH 7.5PMID 19640005Open
thrombinvariant R67C/I82C—H-D-Phe-Pip-Arg-p-nitroanilide0.2 M choline chloride (Na+-free)21.20.01062.00 × 10³ calc.pH 7.5PMID 19640005Open
thrombinHomo sapienscoagulation factor XIII, Leu-34 polymorphic form of the A-subunit activation peptidefibrinogen-enhanced——2.84 × 10³pH 7.4, 37 °CPMID 12794066Open
thrombinHomo sapienscoagulation factor XIII, Leu-34 polymorphic form of the A-subunit activation peptideno cofactor present (direct activation)——330pH 7.4, 37 °CPMID 12794066Open
thrombinHomo sapienscoagulation factor XIII, Val-34 polymorphic form of the A-subunit activation peptidefibrinogen-enhanced——2.29 × 10³pH 7.4, 37 °CPMID 12794066Open
thrombinHomo sapienscoagulation factor XIII, Val-34 polymorphic form of the A-subunit activation peptideno cofactor present (direct activation)——180pH 7.4, 37 °CPMID 12794066Open
thrombinHomo sapiensfibrinogen——1.7 × 10⁴pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensfibrinogen Aα-chain, release of fibrinopeptide A——4.54 × 10³pH 7.4, 37 °CPMID 12794066Open
thrombinHomo sapiensfibrinogen Bβ-chain, release of fibrinopeptide B——1.38 × 10³pH 7.4, 37 °CPMID 12794066Open
thrombinHomo sapiensH-D-Phe-Gly-Arg-p-nitroanilide——520pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Arg-p-nitroanilide200 mM ChCl (Na+-free)3.58.8 × 10⁻⁴4 × 10³pH 8, 25 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Arg-p-nitroanilide200 mM NaCl (Na+-bound)293.3 × 10⁻⁴8.8 × 10⁴pH 8, 25 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Arg-p-nitroanilide——3.7 × 10⁴pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Arg-p-nitroanilidewith 100 nM thrombomodulin and 5 mM CaCl2——3.4 × 10⁴pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Lys-p-nitroanilide200 mM ChCl (Na+-free)120.04300pH 8, 25 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Lys-p-nitroanilide200 mM NaCl (Na+-bound)280.0047 × 10³pH 8, 25 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Lys-p-nitroanilide——4.2 × 10³pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Phe-p-nitroanilide200 mM ChCl (Na+-free)1.40.1113pH 8, 25 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Phe-p-nitroanilide200 mM NaCl (Na+-bound)8.30.017480pH 8, 25 °CPMID 19846563Open
thrombinHomo sapiensH-D-Phe-Pro-Phe-p-nitroanilide——280pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensH-D-phenylalanyl-L-pipecolyl-L-arginine p-nitroanilide0.280.0348.2pH 7.5PMID 39938570Open
thrombinHomo sapiensN-benzyloxycarbonyl-glycyl-glycyl-L-arginine 7-amido-4-methylcoumarin0.230.0822.8pH 7.5PMID 39938570Open
thrombinHomo sapiensprotease-activated receptor 1——3.9 × 10⁴pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensprotease-activated receptor 3——350pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensprotease-activated receptor 4——340pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensprotein Cactivation of protein C; no thrombomodulin——0.059pH 7.4, 37 °CPMID 19846563Open
thrombinHomo sapiensprotein C——220pH 7.4, 37 °CPMID 19846563Open
thrombinvariant H66AHomo sapienscoagulation factor XIII, Leu-34 polymorphic form of the A-subunit activation peptide——580pH 7.4, 37 °CPMID 12794066Open
thrombinvariant H66AHomo sapienscoagulation factor XIII, Val-34 polymorphic form of the A-subunit activation peptide——330pH 7.4, 37 °CPMID 12794066Open
thrombinvariant H66AHomo sapiensfibrinogen Aα-chain, release of fibrinopeptide A——530pH 7.4, 37 °CPMID 12794066Open
thrombinvariant H66AHomo sapiensfibrinogen Bβ-chain, release of fibrinopeptide B——150pH 7.4, 37 °CPMID 12794066Open
thrombinvariant I174AHomo sapiensH-D-phenylalanyl-L-pipecolyl-L-arginine p-nitroanilide0.350.0438.2pH 7.5PMID 39938570Open
thrombinvariant I174AHomo sapiensN-benzyloxycarbonyl-glycyl-glycyl-L-arginine 7-amido-4-methylcoumarin0.130.1630.83pH 7.5PMID 39938570Open
thrombinvariant I174FHomo sapiensH-D-phenylalanyl-L-pipecolyl-L-arginine p-nitroanilide0.40.057.8pH 7.5PMID 39938570Open
thrombinvariant I174FHomo sapiensN-benzyloxycarbonyl-glycyl-glycyl-L-arginine 7-amido-4-methylcoumarin0.10.1530.67pH 7.5PMID 39938570Open
thrombinvariant KL3Homo sapiensH-D-phenylalanyl-L-pipecolyl-L-arginine p-nitroanilide0.580.0827.2pH 7.5PMID 39938570Open
thrombinvariant KL3Homo sapiensN-benzyloxycarbonyl-glycyl-glycyl-L-arginine 7-amido-4-methylcoumarin0.0830.1880.5pH 7.5PMID 39938570Open
thrombinvariant N143PHomo sapiensH-D-Phe-Pro-Arg-p-nitroanilide200 mM ChCl (Na+-free)7.50.013560pH 8, 25 °CPMID 19846563Open
thrombinvariant N143PHomo sapiensH-D-Phe-Pro-Arg-p-nitroanilide200 mM NaCl (Na+-bound)60.00292.1 × 10³pH 8, 25 °CPMID 19846563Open
thrombinvariant N143PHomo sapiensH-D-Phe-Pro-Lys-p-nitroanilide200 mM ChCl (Na+-free)0.260.0723.6pH 8, 25 °CPMID 19846563Open
thrombinvariant N143PHomo sapiensH-D-Phe-Pro-Lys-p-nitroanilide200 mM NaCl (Na+-bound)0.180.006428pH 8, 25 °CPMID 19846563Open
thrombinvariant N143PHomo sapiensH-D-Phe-Pro-Phe-p-nitroanilide200 mM ChCl (Na+-free)0.230.045.8pH 8, 25 °CPMID 19846563Open
thrombinvariant N143PHomo sapiensH-D-Phe-Pro-Phe-p-nitroanilide200 mM NaCl (Na+-bound)0.180.0335.4pH 8, 25 °CPMID 19846563Open
thrombinvariant N74AHomo sapienscoagulation factor XIII, Leu-34 polymorphic form of the A-subunit activation peptide——1.49 × 10³pH 7.4, 37 °CPMID 12794066Open
thrombinvariant N74AHomo sapienscoagulation factor XIII, Val-34 polymorphic form of the A-subunit activation peptide——940pH 7.4, 37 °CPMID 12794066Open
thrombinvariant N74AHomo sapiensfibrinogen Aα-chain, release of fibrinopeptide A——1.78 × 10³pH 7.4, 37 °CPMID 12794066Open
thrombinvariant N74AHomo sapiensfibrinogen Bβ-chain, release of fibrinopeptide B——620pH 7.4, 37 °CPMID 12794066Open
thrombinvariant R233AHomo sapienscoagulation factor XIII, Leu-34 polymorphic form of the A-subunit activation peptide——18pH 7.4, 37 °CPMID 12794066Open
thrombinvariant R233AHomo sapienscoagulation factor XIII, Val-34 polymorphic form of the A-subunit activation peptide——11pH 7.4, 37 °CPMID 12794066Open
thrombinvariant Y71AHomo sapienscoagulation factor XIII, Leu-34 polymorphic form of the A-subunit activation peptide——110pH 7.4, 37 °CPMID 12794066Open
thrombinvariant Y71AHomo sapienscoagulation factor XIII, Val-34 polymorphic form of the A-subunit activation peptide——38pH 7.4, 37 °CPMID 12794066Open
thrombinvariant Y71AHomo sapiensfibrinogen Aα-chain, release of fibrinopeptide A——23pH 7.4, 37 °CPMID 12794066Open
thrombinvariant Y71AHomo sapiensfibrinogen Bβ-chain, release of fibrinopeptide B——11pH 7.4, 37 °CPMID 12794066Open
α-thrombinBos taurusbenzoyl-L-arginine p-nitroanilide0.040.1270.3pH 8PMID 7725319Open
α-thrombinBos taurustosyl-L-arginine methyl ester60.059100pH 8PMID 7725319Open
α-thrombinHomo sapiensH-D-Phe-Pip-Arg-p-nitroanilideno exosite ligand added101.90.00263.98 × 10⁴PMID 19589779Open
α-thrombinHomo sapiensH-D-Phe-Pip-Arg-p-nitroanilidewith 10 μM HD1 (exosite 1-directed DNA aptamer)209.40.01431.47 × 10⁴PMID 19589779Open
α-thrombinHomo sapiensH-D-Phe-Pip-Arg-p-nitroanilidewith 10 μM HD22 (exosite 2-directed DNA aptamer)106.80.01218.8 × 10³PMID 19589779Open
α-thrombinHomo sapiensH-D-Phe-Pip-Arg-p-nitroanilidewith 10 μM hirudin-(54–65) (exosite 1-directed peptide)156.50.00315.1 × 10⁴PMID 19589779Open
α-thrombinHomo sapienspyro-Glu-Pro-Arg-p-nitroanilideno exosite ligand added249.50.2829900PMID 19589779Open
α-thrombinHomo sapienspyro-Glu-Pro-Arg-p-nitroanilidewith 10 μM HD1 (exosite 1-directed DNA aptamer)217.10.1951.1 × 10³PMID 19589779Open
α-thrombinHomo sapienspyro-Glu-Pro-Arg-p-nitroanilidewith 10 μM HD22 (exosite 2-directed DNA aptamer)231.10.15541.5 × 10³PMID 19589779Open
α-thrombinHomo sapienspyro-Glu-Pro-Arg-p-nitroanilidewith 10 μM hirudin-(54–65) (exosite 1-directed peptide)278.60.14711.9 × 10³PMID 19589779Open
α-thrombinHomo sapienstosyl-Gly-Pro-Arg-p-nitroanilide (tosyl-Gly-Pro-Arg-p-nitroaniline acetate)no exosite ligand added234.50.01641.43 × 10⁴PMID 19589779Open
α-thrombinHomo sapienstosyl-Gly-Pro-Arg-p-nitroanilide (tosyl-Gly-Pro-Arg-p-nitroaniline acetate)with 10 μM HD1 (exosite 1-directed DNA aptamer)240.30.01421.69 × 10⁴PMID 19589779Open
α-thrombinHomo sapienstosyl-Gly-Pro-Arg-p-nitroanilide (tosyl-Gly-Pro-Arg-p-nitroaniline acetate)with 10 μM HD22 (exosite 2-directed DNA aptamer)192.80.00852.27 × 10⁴PMID 19589779Open
α-thrombinHomo sapienstosyl-Gly-Pro-Arg-p-nitroanilide (tosyl-Gly-Pro-Arg-p-nitroaniline acetate)with 10 μM hirudin-(54–65) (exosite 1-directed peptide)251.60.01172.15 × 10⁴PMID 19589779Open