Values CatLog read from this paper

The mechanism of the reaction catalyzed by uronate isomerase illustrates how an isomerase may have evolved from a hydrolase within the amidohydrolase superfamily

PMID 19678710doi:10.1021/bi901046xOpen in Browse

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CatLog read 22 records (66 values) from this paper: uronate isomerase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 5.3.1.12

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
uronate isomerase	Escherichia coli	D-glucuronate	196	0.5	400	pH 8, 30 °C	Open record: uronate isomerase, D-glucuronate
uronate isomerasevariant D238N	Escherichia coli	D-glucuronate	60	1.3	46	pH 8, 30 °C	Open record: uronate isomerase variant D238N, D-glucuronate
uronate isomerasevariant D412A	Escherichia coli	D-glucuronate	0.009	0.4	0.021	pH 8, 30 °C	Open record: uronate isomerase variant D412A, D-glucuronate
uronate isomerasevariant D412N	Escherichia coli	D-glucuronate	0.6	1	0.6	pH 8, 30 °C	Open record: uronate isomerase variant D412N, D-glucuronate
uronate isomerasevariant H297A	Escherichia coli	D-glucuronate	10	220	0.043	pH 8, 30 °C	Open record: uronate isomerase variant H297A, D-glucuronate
uronate isomerasevariant H297N	Escherichia coli	D-glucuronate	30	56	0.5	pH 8, 30 °C	Open record: uronate isomerase variant H297N, D-glucuronate
uronate isomerasevariant H33A	Escherichia coli	D-glucuronate	0.6	0.2	3	pH 8, 30 °C	Open record: uronate isomerase variant H33A, D-glucuronate
uronate isomerasevariant H33N	Escherichia coli	D-glucuronate	2.1	0.05	47	pH 8, 30 °C	Open record: uronate isomerase variant H33N, D-glucuronate
uronate isomerasevariant H35A	Escherichia coli	D-glucuronate	0.7	39	0.018	pH 8, 30 °C	Open record: uronate isomerase variant H35A, D-glucuronate
uronate isomerasevariant H35N	Escherichia coli	D-glucuronate	4	9.4	0.43	pH 8, 30 °C	Open record: uronate isomerase variant H35N, D-glucuronate
uronate isomerasevariant H59A	Escherichia coli	D-glucuronate	0.6	0.7	0.83	pH 8, 30 °C	Open record: uronate isomerase variant H59A, D-glucuronate
uronate isomerasevariant H59N	Escherichia coli	D-glucuronate	15	0.7	21	pH 8, 30 °C	Open record: uronate isomerase variant H59N, D-glucuronate
uronate isomerasevariant R186K	Escherichia coli	D-glucuronate	54	2.6	21	pH 8, 30 °C	Open record: uronate isomerase variant R186K, D-glucuronate
uronate isomerasevariant R186M	Escherichia coli	D-glucuronate	4.7	38	0.13	pH 8, 30 °C	Open record: uronate isomerase variant R186M, D-glucuronate
uronate isomerasevariant R302K	Escherichia coli	D-glucuronate	160	2.5	63	pH 8, 30 °C	Open record: uronate isomerase variant R302K, D-glucuronate
uronate isomerasevariant R302M	Escherichia coli	D-glucuronate	180	200	0.88	pH 8, 30 °C	Open record: uronate isomerase variant R302M, D-glucuronate
uronate isomerasevariant R414K	Escherichia coli	D-glucuronate	5.8	0.82	7.1	pH 8, 30 °C	Open record: uronate isomerase variant R414K, D-glucuronate
uronate isomerasevariant R414M	Escherichia coli	D-glucuronate	0.7	1.4	0.54	pH 8, 30 °C	Open record: uronate isomerase variant R414M, D-glucuronate
uronate isomerasevariant W381A	Escherichia coli	D-glucuronate	250	21	12	pH 8, 30 °C	Open record: uronate isomerase variant W381A, D-glucuronate
uronate isomerasevariant W381F	Escherichia coli	D-glucuronate	16	1.7	9.5	pH 8, 30 °C	Open record: uronate isomerase variant W381F, D-glucuronate
uronate isomerasevariant Y60A	Escherichia coli	D-glucuronate	13.9	0.21	66	pH 8, 30 °C	Open record: uronate isomerase variant Y60A, D-glucuronate
uronate isomerasevariant Y60F	Escherichia coli	D-glucuronate	21.7	0.16	140	pH 8, 30 °C	Open record: uronate isomerase variant Y60F, D-glucuronate