Values CatLog read from this paper
Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6
PMID 19788271doi:10.1021/bi9013825Open in Browse
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CatLog read 18 records (52 values) from this paper: endo-α-N-acetylgalactosaminidase (SpGH101) from Streptococcus pneumoniae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| endo-α-N-acetylgalactosaminidase (SpGH101) | Streptococcus pneumoniae | 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside | 1.6 | 2.4 | 0.6 | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101), 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101) | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 541 | 0.034 | 1.59 × 10⁴ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101), 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101) | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 748 | 0.048 | 1.56 × 10⁴ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101), 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101) | Streptococcus pneumoniae | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 579 | 0.062 | 9.3 × 10³ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant D658A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | — | — | 987 | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant D658A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant D764A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 0.76 | 0.033 | 23 | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant D764A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside | 5 | 2.1 | 2 | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 18 | 0.0013 | 1.38 × 10⁴ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside100 mM NaN3 (azide; anion rescue) | 33 | 0.0056 | 5.9 × 10³ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside100 mM NaCl (salt control) | 16.6 | 0.0015 | 1.1 × 10⁴ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside500 mM sodium formate (anion rescue) | 48 | 0.011 | 4.4 × 10³ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside500 mM NaCl (salt control) | 14 | 0.0016 | 8.75 × 10³ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 1 | 0.021 | 48 | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 14 | 0.0012 | 1.17 × 10⁴ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranosideNaN3 (azide; anion rescue) | 74 | 0.015 | 4.9 × 10³ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranosidesodium formate (anion rescue) | 32 | 0.0097 | 3.3 × 10³ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranosideNaCl (salt control) | 9.1 | 0.0017 | 5.4 × 10³ | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 2.1 | 0.212 | 9.9 | pH 6.5, 37 °C | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |