EC 3.2.1.97: endo-alpha-N-acetylgalactosaminidase
HydrolasesOpen in Browse
CatLog read 43 records for EC 3.2.1.97 from 3 papers, for 3 organisms. Names in these papers: endo-α-N-acetylgalactosaminidase (EngBF), endo-α-N-acetylgalactosaminidase (SpGH101), endo-alpha-N-acetylgalactosaminidase (EngBF).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 17 records for EC 3.2.1.97 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.2.1.97 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Identification of a Catalytic Nucleophile-Activating Network in the endo-α-N-Acetylgalactosaminidase of Family GH101 | Bifidobacterium longum | 19 |
| Mechanistic investigation of the endo-alpha-N-acetylgalactosaminidase from Streptococcus pneumoniae R6 | Streptococcus pneumoniae | 18 |
| Steady state kinetic analysis of O-linked GalNAc glycan release catalyzed by endo-α-N-acetylgalactosaminidase | Bifidobacterium longum subsp. longum | 6 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| endo-alpha-N-acetylgalactosaminidase (EngBF) | Bifidobacterium longum subsp. longum | sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) | 207 | 0.0987 | 2.1 × 10³ | pH 4.5, 37 °C | PMID 31176190 | Open record: endo-alpha-N-acetylgalactosaminidase (EngBF), sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) |
| endo-alpha-N-acetylgalactosaminidase (EngBF) | Bifidobacterium longum subsp. longum | sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) | — | — | 109 | pH 7, 37 °C | PMID 31176190 | Open record: endo-alpha-N-acetylgalactosaminidase (EngBF), sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) |
| endo-alpha-N-acetylgalactosaminidase (EngBF)variant D1295A | Bifidobacterium longum subsp. longum | sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) | 55.7 | 0.131 | 430 | pH 4.5, 37 °C | PMID 31176190 | Open record: endo-alpha-N-acetylgalactosaminidase (EngBF) variant D1295A, sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) |
| endo-alpha-N-acetylgalactosaminidase (EngBF)variant K1199A | Bifidobacterium longum subsp. longum | sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) | 133 | 0.167 | 800 | pH 4.5, 37 °C | PMID 31176190 | Open record: endo-alpha-N-acetylgalactosaminidase (EngBF) variant K1199A, sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) |
| endo-alpha-N-acetylgalactosaminidase (EngBF)variant Q894A | Bifidobacterium longum subsp. longum | sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) | 157 | 0.128 | 1.22 × 10³ | pH 4.5, 37 °C | PMID 31176190 | Open record: endo-alpha-N-acetylgalactosaminidase (EngBF) variant Q894A, sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) |
| endo-alpha-N-acetylgalactosaminidase (EngBF)variant W750A | Bifidobacterium longum subsp. longum | sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) | 13.2 | 0.398 | 33 | pH 4.5, 37 °C | PMID 31176190 | Open record: endo-alpha-N-acetylgalactosaminidase (EngBF) variant W750A, sialidase-treated bovine kappa-casein glycomacropeptide (asialo-CGMP; released glycan Galbeta(1-3)GalNAc) |
| endo-α-N-acetylgalactosaminidase (EngBF) | Bifidobacterium longum | asialo-casein glycomacropeptide | 194 | 0.114 | 1.7 × 10³ | pH 4.5 | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF), asialo-casein glycomacropeptide |
| endo-α-N-acetylgalactosaminidase (EngBF) | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 96.5 | 0.0211 | 4.58 × 10³ | pH 6, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF), Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF) | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 141.8 | 0.0224 | 6.4 × 10³ | pH 5.5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF), Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF) | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 155.6 | 0.0232 | 6.7 × 10³ | pH 5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF), Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF) | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 167 | 0.0482 | 3.46 × 10³ | pH 4.5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF), Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685A | Bifidobacterium longum | asialo-casein glycomacropeptide | 50 | 0.05 | 1.04 × 10³ | pH 4.5 | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685A, asialo-casein glycomacropeptide |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718A | Bifidobacterium longum | asialo-casein glycomacropeptide | 0.73 | 0.072 | 10 | pH 4.5 | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685A/H718A, asialo-casein glycomacropeptide |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718A | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 13.6 | 3.079 | 4.4 | pH 6, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685A/H718A, Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718A | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 13.6 | 2.163 | 6.3 | pH 5.5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685A/H718A, Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718A | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 8.4 | 1.486 | 5.6 | pH 5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685A/H718A, Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718A | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 7.3 | 2.33 | 3.1 | pH 4.5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685A/H718A, Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q | Bifidobacterium longum | asialo-casein glycomacropeptide | 335 | 0.149 | 2.25 × 10³ | pH 4.5 | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685Q, asialo-casein glycomacropeptide |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718Q | Bifidobacterium longum | asialo-casein glycomacropeptide | 0.78 | 0.157 | 4.9 | pH 4.5 | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685Q/H718Q, asialo-casein glycomacropeptide |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718Q | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 16.5 | 0.757 | 22 | pH 6, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685Q/H718Q, Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718Q | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 21.5 | 0.956 | 22 | pH 5.5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685Q/H718Q, Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718Q | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 18.9 | 1.916 | 9.9 | pH 5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685Q/H718Q, Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718Q | Bifidobacterium longum | Galβ(1−3)GalNAcα1-para-nitrophenol | 10.7 | 2.348 | 4.6 | pH 4.5, 37 °C | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H685Q/H718Q, Galβ(1−3)GalNAcα1-para-nitrophenol |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H718A | Bifidobacterium longum | asialo-casein glycomacropeptide | 13.6 | 0.0275 | 495 | pH 4.5 | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H718A, asialo-casein glycomacropeptide |
| endo-α-N-acetylgalactosaminidase (EngBF)variant H718Q | Bifidobacterium longum | asialo-casein glycomacropeptide | 38.2 | 0.0375 | 1.02 × 10³ | pH 4.5 | PMID 34694774 | Open record: endo-α-N-acetylgalactosaminidase (EngBF) variant H718Q, asialo-casein glycomacropeptide |
| endo-α-N-acetylgalactosaminidase (SpGH101) | Streptococcus pneumoniae | 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside | 1.6 | 2.4 | 0.6 | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101), 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101) | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 541 | 0.034 | 1.59 × 10⁴ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101), 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101) | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 748 | 0.048 | 1.56 × 10⁴ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101), 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101) | Streptococcus pneumoniae | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 579 | 0.062 | 9.3 × 10³ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101), 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant D658A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | — | — | 987 | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant D658A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant D764A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 0.76 | 0.033 | 23 | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant D764A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside | 5 | 2.1 | 2 | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 18 | 0.0013 | 1.38 × 10⁴ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside100 mM NaN3 (azide; anion rescue) | 33 | 0.0056 | 5.9 × 10³ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside100 mM NaCl (salt control) | 16.6 | 0.0015 | 1.1 × 10⁴ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside500 mM sodium formate (anion rescue) | 48 | 0.011 | 4.4 × 10³ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside500 mM NaCl (salt control) | 14 | 0.0016 | 8.75 × 10³ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796A | Streptococcus pneumoniae | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 1 | 0.021 | 48 | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796A, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 14 | 0.0012 | 1.17 × 10⁴ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranosideNaN3 (azide; anion rescue) | 74 | 0.015 | 4.9 × 10³ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranosidesodium formate (anion rescue) | 32 | 0.0097 | 3.3 × 10³ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranosideNaCl (salt control) | 9.1 | 0.0017 | 5.4 × 10³ | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 2,4-dinitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |
| endo-α-N-acetylgalactosaminidase (SpGH101)variant E796Q | Streptococcus pneumoniae | 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside | 2.1 | 0.212 | 9.9 | pH 6.5, 37 °C | PMID 19788271 | Open record: endo-α-N-acetylgalactosaminidase (SpGH101) variant E796Q, 4-nitrophenyl beta-D-galactopyranosyl-(1->3)-2-acetamido-2-deoxy-alpha-D-galactopyranoside |