CatLogenzyme kinetics catalog

Values CatLog read from this paper

Identification of a Catalytic Nucleophile-Activating Network in the endo-α-N-Acetylgalactosaminidase of Family GH101

PMID 34694774doi:10.1021/acs.biochem.1c00596Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (57 values) from this paper: endo-α-N-acetylgalactosaminidase (EngBF) from Bifidobacterium longum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.97

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
endo-α-N-acetylgalactosaminidase (EngBF)Bifidobacterium longumasialo-casein glycomacropeptide1940.1141.7 × 10³pH 4.5Open
endo-α-N-acetylgalactosaminidase (EngBF)Bifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol96.50.02114.58 × 10³pH 6, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)Bifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol141.80.02246.4 × 10³pH 5.5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)Bifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol155.60.02326.7 × 10³pH 5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)Bifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol1670.04823.46 × 10³pH 4.5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H685ABifidobacterium longumasialo-casein glycomacropeptide500.051.04 × 10³pH 4.5Open
endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718ABifidobacterium longumasialo-casein glycomacropeptide0.730.07210pH 4.5Open
endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718ABifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol13.63.0794.4pH 6, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718ABifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol13.62.1636.3pH 5.5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718ABifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol8.41.4865.6pH 5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H685A/H718ABifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol7.32.333.1pH 4.5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H685QBifidobacterium longumasialo-casein glycomacropeptide3350.1492.25 × 10³pH 4.5Open
endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718QBifidobacterium longumasialo-casein glycomacropeptide0.780.1574.9pH 4.5Open
endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718QBifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol16.50.75722pH 6, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718QBifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol21.50.95622pH 5.5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718QBifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol18.91.9169.9pH 5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H685Q/H718QBifidobacterium longumGalβ(1−3)GalNAcα1-para-nitrophenol10.72.3484.6pH 4.5, 37 °COpen
endo-α-N-acetylgalactosaminidase (EngBF)variant H718ABifidobacterium longumasialo-casein glycomacropeptide13.60.0275495pH 4.5Open
endo-α-N-acetylgalactosaminidase (EngBF)variant H718QBifidobacterium longumasialo-casein glycomacropeptide38.20.03751.02 × 10³pH 4.5Open