CatLogenzyme kinetics catalog

Values CatLog read from this paper

Probing the catalytic potential of the hamster arylamine N-acetyltransferase 2 catalytic triad by site-directed mutagenesis of the proximal conserved residue, Tyr190

PMID 19860825doi:10.1111/j.1742-4658.2009.07389.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 33 records (78 values) from this paper: arylamine N-acetyltransferase 2 (NAT2) from hamster.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.3.1.5

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
arylamine N-acetyltransferase 2 (NAT2)variant Y190AhamsterAcCoAPNA acetylation followed at 430; cosubstrate PNA—1.490.17pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190AhamsterAcCoAresidual PABA quantified as the DMAB Schiff base at 450—2.73pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ahamsteranisidine280.25114pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ahamsterp-aminobenzoic acidPNPA as acetyl donor; MOPS buffer 100 mM; 150 mM NaCl380.11345pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ahamsterp-aminobenzoic acidAcCoA as acetyl donor; dimethylglutaric acid buffer 50 mM8.140.2829pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ahamsterp-aminobenzoyl-glutamic acid166.22.6pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ahamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; Table 2—7.43.8pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ahamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; cosubstrate PABA—7.55pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ahamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; Table 2—5.62.8pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ahamsterp-nitrophenyl acetatesingle-turnover stopped-flow acetylation by PNPA; 3% DMSO—2.8—pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190AhamsterPNA0.252.920.087pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190FhamsterAcCoAPNA acetylation followed at 430; cosubstrate PNA—0.142.23pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190FhamsterAcCoAresidual PABA quantified as the DMAB Schiff base at 450—1.8106pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Fhamsteranisidine2880.58496pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Fhamsterp-aminobenzoic acidPNPA as acetyl donor; MOPS buffer 100 mM; 150 mM NaCl3930.221786pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Fhamsterp-aminobenzoic acidAcCoA as acetyl donor; dimethylglutaric acid buffer 50 mM1890.141.36 × 10³pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Fhamsterp-aminobenzoyl-glutamic acid862.534pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Fhamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; Table 2—7.837pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Fhamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; cosubstrate PABA—7.751pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Fhamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; Table 2—2.830pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Fhamsterp-nitrophenyl acetatesingle-turnover stopped-flow acetylation by PNPA; 3% DMSO—3.5—pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190FhamsterPNA0.310.480.66pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190IhamsterAcCoAPNA acetylation followed at 430; cosubstrate PNA—0.711.26pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190IhamsterAcCoAresidual PABA quantified as the DMAB Schiff base at 450—1.930pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ihamsteranisidine850.18483pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ihamsterp-aminobenzoic acidPNPA as acetyl donor; MOPS buffer 100 mM; 150 mM NaCl600.08746pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ihamsterp-aminobenzoic acidAcCoA as acetyl donor; dimethylglutaric acid buffer 50 mM580.06974pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ihamsterp-aminobenzoyl-glutamic acid772.728pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ihamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; Table 2—10.18.4pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ihamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; cosubstrate PABA—4.513pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ihamsterp-nitrophenyl acetatePNPA as acetyl donor; 0.1 mM DTT; 4% DMSO; Table 2—117pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190Ihamsterp-nitrophenyl acetatesingle-turnover stopped-flow acetylation by PNPA; 3% DMSO—4.1—pH 7, 25 °COpen
arylamine N-acetyltransferase 2 (NAT2)variant Y190IhamsterPNA0.891.510.6pH 7, 25 °COpen