Values CatLog read from this paper

Exploring the substrate promiscuity of drug-modifying enzymes for the chemoenzymatic generation of N-acylated aminoglycosides

PMID 19899089doi:10.1002/cbic.200900584Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 43 records (129 values) from this paper: AAC(6’)-APH(2’’), AAC(3)-IV.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.3.1.81

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
AAC(3)-IV	—	acetoacetyl-coenzyme A	0.088	0.0918	1	pH 5.7	Open record: AAC(3)-IV, acetoacetyl-coenzyme A
AAC(3)-IV	—	acetyl-coenzyme Avaried acetyl-CoA at fixed gentamicin	0.043	8.03 × 10⁻⁴	53	pH 5.7	Open record: AAC(3)-IV, acetyl-coenzyme A
AAC(3)-IV	—	acetyl-coenzyme Avaried acetyl-CoA at fixed gentamicin	0.104	6.8 × 10⁻⁴	152	pH 5.7	Open record: AAC(3)-IV, acetyl-coenzyme A
AAC(3)-IV	—	acetyl-coenzyme Avaried acetyl-CoA at fixed neomycin B	0.35	0.00917	39	pH 5.7	Open record: AAC(3)-IV, acetyl-coenzyme A
AAC(3)-IV	—	acetyl-coenzyme Avaried acetyl-CoA at fixed paromomycin	0.13	0.0037	35	pH 6.6	Open record: AAC(3)-IV, acetyl-coenzyme A
AAC(3)-IV	—	acetyl-coenzyme Avaried acetyl-CoA at fixed sisomicin; cosubstrate sisomicin	0.057	0.00205	28	pH 5.7	Open record: AAC(3)-IV, acetyl-coenzyme A
AAC(3)-IV	—	acetyl-coenzyme Avaried acetyl-CoA at fixed tobramycin	0.045	0.00376	12	pH 6.6	Open record: AAC(3)-IV, acetyl-coenzyme A
AAC(3)-IV	—	benzoyl-coenzyme A	0.02	0.0231	1	pH 5.7	Open record: AAC(3)-IV, benzoyl-coenzyme A
AAC(3)-IV	—	gentamicin	0.036	8.9 × 10⁻⁴	40	pH 5.7	Open record: AAC(3)-IV, gentamicin
AAC(3)-IV	—	malonyl-coenzyme A	0.189	0.0465	4	pH 6.6	Open record: AAC(3)-IV, malonyl-coenzyme A
AAC(3)-IV	—	n-propionyl-coenzyme Agentamicin; cosubstrate gentamicin	0.037	0.0946	0.4	pH 5.7	Open record: AAC(3)-IV, n-propionyl-coenzyme A
AAC(3)-IV	—	n-propionyl-coenzyme Agentamicin; cosubstrate gentamicin	0.025	0.0788	0.3	pH 5.7	Open record: AAC(3)-IV, n-propionyl-coenzyme A
AAC(3)-IV	—	n-propionyl-coenzyme Aneomycin B; cosubstrate neomycin B	0.045	0.0249	1.8	pH 5.7	Open record: AAC(3)-IV, n-propionyl-coenzyme A
AAC(3)-IV	—	n-propionyl-coenzyme Aparomomycin; cosubstrate paromomycin	0.075	0.0469	1.6	pH 6.6	Open record: AAC(3)-IV, n-propionyl-coenzyme A
AAC(3)-IV	—	n-propionyl-coenzyme Asisomicin; cosubstrate sisomicin	0.032	0.0151	2	pH 5.7	Open record: AAC(3)-IV, n-propionyl-coenzyme A
AAC(3)-IV	—	n-propionyl-coenzyme Atobramycin; cosubstrate tobramycin	0.69	0.043	16	pH 6.6	Open record: AAC(3)-IV, n-propionyl-coenzyme A
AAC(3)-IV	—	neomycin B	0.045	5.6 × 10⁻⁴	82	pH 5.7	Open record: AAC(3)-IV, neomycin B
AAC(3)-IV	—	paromomycin	0.29	0.00126	23	pH 6.6	Open record: AAC(3)-IV, paromomycin
AAC(3)-IV	—	sisomicin	0.026	3.5 × 10⁻⁴	74	pH 5.7	Open record: AAC(3)-IV, sisomicin
AAC(3)-IV	—	tobramycin	0.12	0.00187	66	pH 6.6	Open record: AAC(3)-IV, tobramycin
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed kanamycin A	0.012	0.002	24	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed kanamycin A	0.163	0.0033	50	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed kanamycin A	0.559	0.0099	56	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed kanamycin A	0.3	0.0055	55	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed kanamycin A	0.075	0.0028	27	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed amikacin; cosubstrate amikacin	0.126	9.6 × 10⁻⁴	131	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed gentamicin	0.182	0.0046	40	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed neomycin B	0.188	0.0148	13	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed sisomicin; cosubstrate sisomicin	0.173	0.0015	112	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	acetyl-coenzyme Avaried acetyl-CoA at fixed tobramycin	0.179	0.0027	66	pH 6.6	Open record: AAC(6’)-APH(2’’), acetyl-coenzyme A
AAC(6’)-APH(2’’)	—	kanamycin A	0.034	4 × 10⁻⁴	87	pH 6.6	Open record: AAC(6’)-APH(2’’), kanamycin A
AAC(6’)-APH(2’’)	—	malonyl-coenzyme Avaried malonyl-CoA at fixed amikacin; cosubstrate amikacin	0.146	0.0716	2	pH 6.6	Open record: AAC(6’)-APH(2’’), malonyl-coenzyme A
AAC(6’)-APH(2’’)	—	malonyl-coenzyme Avaried malonyl-CoA at fixed gentamicin	0.041	0.0189	2	pH 6.6	Open record: AAC(6’)-APH(2’’), malonyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Akanamycin A; cosubstrate kanamycin A	0.017	0.0048	3	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Akanamycin A; cosubstrate kanamycin A	0.044	0.0091	5	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Akanamycin A; cosubstrate kanamycin A	0.144	0.0135	11	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Akanamycin A; cosubstrate kanamycin A	0.134	0.0111	12	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Aamikacin; cosubstrate amikacin; C-terminal His6 tag (pET22b)	0.124	0.0021	60	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Agentamicin; cosubstrate gentamicin	0.123	0.0029	43	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Aneomycin B; cosubstrate neomycin B	0.076	0.0031	24	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Asisomicin; cosubstrate sisomicin	0.121	5.7 × 10⁻⁴	211	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	n-propionyl-coenzyme Atobramycin; cosubstrate tobramycin	0.089	0.0054	16	pH 6.6	Open record: AAC(6’)-APH(2’’), n-propionyl-coenzyme A
AAC(6’)-APH(2’’)	—	sisomicin	0.382	0.0059	65	pH 6.6	Open record: AAC(6’)-APH(2’’), sisomicin