Values CatLog read from this paper
Characterization of site-directed mutants of residues R58, R59, D116, W340 and R372 in the active site of E. coli cystathionine beta-lyase
PMID 20014435doi:10.1002/pro.308Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (27 values) from this paper: cystathionine beta-lyase (eCBL) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cystathionine beta-lyase (eCBL) | Escherichia coli | L-cystathionine | 34.1 | 0.18 | 190 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL), L-cystathionine |
| cystathionine beta-lyase (eCBL)variant D116A | Escherichia coli | L-cystathionine | 41.9 | 0.67 | 62 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant D116A, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant D116N | Escherichia coli | L-cystathionine | 37.9 | 0.55 | 69 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant D116N, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant R372A | Escherichia coli | L-cystathionine | — | — | 9 × 10⁻⁵ | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant R372A, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant R372K | Escherichia coli | L-cystathionine | 8.1 | 15 | 0.55 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant R372K, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant R372L | Escherichia coli | L-cystathionine | — | — | 2.3 × 10⁻⁴ | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant R372L, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant R58A | Escherichia coli | L-cystathionine | 9.7 | 5.1 | 1.92 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant R58A, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant R58K | Escherichia coli | L-cystathionine | — | — | 0.385 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant R58K, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant R59A | Escherichia coli | L-cystathionine | 45.8 | 1.02 | 45 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant R59A, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant R59K | Escherichia coli | L-cystathionine | 36.7 | 1.51 | 24 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant R59K, L-cystathionine |
| cystathionine beta-lyase (eCBL)variant W340F | Escherichia coli | L-cystathionine | 79 | 1.35 | 59 | pH 8.5, 25 °C | Open record: cystathionine beta-lyase (eCBL) variant W340F, L-cystathionine |