CatLogenzyme kinetics catalog

EC 4.4.1.28: L-cysteine desulfidase

LyasesOpen in Browse

CatLog read 53 records for EC 4.4.1.28 from 5 papers, for 2 organisms. Names in these papers: cystathionine beta-lyase (eCBL), cystathionine β-lyase (eCBL), C-S lyase (LDB C-S lyase).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 15 records for EC 4.4.1.28 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.4.1.28 in Browse.

Papers

PaperOrganismsRecords
Exploration of the six tryptophan residues of Escherichia coli cystathionine β-lyase as probes of enzyme conformational changeEscherichia coli18
Characterization of site-directed mutants of residues R58, R59, D116, W340 and R372 in the active site of E. coli cystathionine beta-lyaseEscherichia coli11
Exploration of structure-function relationships in Escherichia coli cystathionine γ-synthase and cystathionine β-lyase via chimeric constructs and site-specific substitutionsEscherichia coli11
Characterization of C-S lyase from Lactobacillus delbrueckii subsp. bulgaricus ATCC BAA-365 and its potential role in food flavour applicationsLactobacillus delbrueckii subsp. bulgaricus8
Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase, and Saccharomyces cerevisiae cystathionine gamma-lyase and…Escherichia coli5

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
C-S lyase (LDB C-S lyase)Lactobacillus delbrueckii subsp. bulgaricusL-cystathionine1143pH 8.3, 25 °CPMID 28003427Open
C-S lyase (LDB C-S lyase)Lactobacillus delbrueckii subsp. bulgaricusL-cysteine2.90.47pH 8.3, 25 °CPMID 28003427Open
C-S lyase (LDB C-S lyase)Lactobacillus delbrueckii subsp. bulgaricusL-cystine292.114pH 8.3, 25 °CPMID 28003427Open
C-S lyase (LDB C-S lyase)Lactobacillus delbrueckii subsp. bulgaricusL-djenkolic acid190.4840pH 8.3, 25 °CPMID 28003427Open
C-S lyase (LDB C-S lyase)Lactobacillus delbrueckii subsp. bulgaricusL-serine0.150.02pH 8.3, 25 °CPMID 28003427Open
C-S lyase (LDB C-S lyase)Lactobacillus delbrueckii subsp. bulgaricusO-acetyl-L-serine1782.2pH 8.3, 25 °CPMID 28003427Open
C-S lyase (LDB C-S lyase)Lactobacillus delbrueckii subsp. bulgaricusS-(2-aminoethyl)-L-cysteine17.80.2766pH 8.3, 25 °CPMID 28003427Open
C-S lyase (LDB C-S lyase)Lactobacillus delbrueckii subsp. bulgaricusS-(2-methyl-4-oxopentan-2-yl)-L-cysteine40.58pH 8.3, 25 °CPMID 28003427Open
cystathionine beta-lyase (eCBL)Escherichia coliL-cystathionine34.10.18190pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)Escherichia coliL-cystathionine54.50.116470pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant D116AEscherichia coliL-cystathionine41.90.6762pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant D116NEscherichia coliL-cystathionine37.90.5569pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant R372AEscherichia coliL-cystathionine——9 × 10⁻⁵pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant R372KEscherichia coliL-cystathionine8.1150.55pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant R372LEscherichia coliL-cystathionine——2.3 × 10⁻⁴pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant R58AEscherichia coliL-cystathionine9.75.11.92pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant R58KEscherichia coliL-cystathionine——0.385pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant R59AEscherichia coliL-cystathionine45.81.0245pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant R59KEscherichia coliL-cystathionine36.71.5124pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant W131FEscherichia coliL-cystathionine45.30.101450pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W188F/W230F/W276FEscherichia coliL-cystathionine25.40.2794pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W188F/W230F/W276F/W300FEscherichia coliL-cystathionine31.70.148213pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W188F/W230F/W276F/W300F/W340FEscherichia coliL-cystathionine461.825pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W188F/W230F/W276F/W340FEscherichia coliL-cystathionine401.823pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W188F/W230F/W300F/W340FEscherichia coliL-cystathionine382.118pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W188F/W276F/W300F/W340FEscherichia coliL-cystathionine35.11.0533pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W230F/W276FEscherichia coliL-cystathionine54.50.171320pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W230F/W276F/W300FEscherichia coliL-cystathionine740.31240pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W230F/W276F/W300F/W340FEscherichia coliL-cystathionine541.2643pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W131F/W230F/W276F/W340FEscherichia coliL-cystathionine65166pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W188FEscherichia coliL-cystathionine38.10.121315pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W188F/W230F/W276F/W300F/W340FEscherichia coliL-cystathionine42221pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W230FEscherichia coliL-cystathionine55.90.19300pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W276FEscherichia coliL-cystathionine60.40.131460pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W300FEscherichia coliL-cystathionine42.50.161260pH 8.5, 25 °CPMID 23969077Open
cystathionine beta-lyase (eCBL)variant W340FEscherichia coliL-cystathionine791.3559pH 8.5, 25 °CPMID 20014435Open
cystathionine beta-lyase (eCBL)variant W340FEscherichia coliL-cystathionine60.70.9166pH 8.5, 25 °CPMID 23969077Open
cystathionine β-lyase (eCBL)Escherichia colicystathionine45.30.108420pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)Escherichia coliL-cystathionine350.23152pH 8.5, 25 °CPMID 19370061Open
cystathionine β-lyase (eCBL)Escherichia coliL-cystathionine230.18127pH 8.5, 25 °CPMID 19370061Open
cystathionine β-lyase (eCBL)Escherichia coliO-acetyl-L-serine0.572.90.2pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)chimera eCGS–sC–eCBL (eCBL region 2, residues 347–372, replaced by eCGS residues 334–361)Escherichia colicystathionine6.83.81.76pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)chimera eCGS–sC–eCBL (eCBL region 2, residues 347–372, replaced by eCGS residues 334–361)Escherichia coliO-acetyl-L-serine0.01990.0021pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)chimera eCGS–sN–eCBL (eCBL region 1, residues 36–56, replaced by eCGS residues 35–46)Escherichia colicystathionine——0.0098pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)variant F55DEscherichia coliL-cystathionine24141.7pH 8.5, 25 °CPMID 19370061Open
cystathionine β-lyase (eCBL)variant F55D/Y338EEscherichia coliL-cystathionine——0.0022pH 8.5, 25 °CPMID 19370061Open
cystathionine β-lyase (eCBL)variant K42AEscherichia colicystathionine40.70.22190pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)variant K42AEscherichia coliO-acetyl-L-serine0.8110.069pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)variant S32AEscherichia colicystathionine6.780.06899pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)variant S32AEscherichia coliO-acetyl-L-serine0.7140.178pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)variant S33AEscherichia colicystathionine4.240.10441pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)variant S33AEscherichia coliO-acetyl-L-serine0.0761.080.07pH 8.5, 25 °CPMID 23470500Open
cystathionine β-lyase (eCBL)variant Y338EEscherichia coliL-cystathionine——0.0445pH 8.5, 25 °CPMID 19370061Open