Values CatLog read from this paper
Exploration of structure-function relationships in Escherichia coli cystathionine γ-synthase and cystathionine β-lyase via chimeric constructs and site-specific substitutions
PMID 23470500doi:10.1016/j.bbapap.2013.02.036Open in Browse
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CatLog read 11 records (31 values) from this paper: cystathionine β-lyase (eCBL) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cystathionine β-lyase (eCBL) | Escherichia coli | cystathionine | 45.3 | 0.108 | 420 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL), cystathionine |
| cystathionine β-lyase (eCBL) | Escherichia coli | O-acetyl-L-serine | 0.57 | 2.9 | 0.2 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL), O-acetyl-L-serine |
| cystathionine β-lyase (eCBL)chimera eCGS–sC–eCBL (eCBL region 2, residues 347–372, replaced by eCGS residues 334–361) | Escherichia coli | cystathionine | 6.8 | 3.8 | 1.76 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) chimera eCGS–sC–eCBL (eCBL region 2, residues 347–372, replaced by eCGS residues 334–361), cystathionine |
| cystathionine β-lyase (eCBL)chimera eCGS–sC–eCBL (eCBL region 2, residues 347–372, replaced by eCGS residues 334–361) | Escherichia coli | O-acetyl-L-serine | 0.019 | 9 | 0.0021 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) chimera eCGS–sC–eCBL (eCBL region 2, residues 347–372, replaced by eCGS residues 334–361), O-acetyl-L-serine |
| cystathionine β-lyase (eCBL)chimera eCGS–sN–eCBL (eCBL region 1, residues 36–56, replaced by eCGS residues 35–46) | Escherichia coli | cystathionine | — | — | 0.0098 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) chimera eCGS–sN–eCBL (eCBL region 1, residues 36–56, replaced by eCGS residues 35–46), cystathionine |
| cystathionine β-lyase (eCBL)variant K42A | Escherichia coli | cystathionine | 40.7 | 0.22 | 190 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant K42A, cystathionine |
| cystathionine β-lyase (eCBL)variant K42A | Escherichia coli | O-acetyl-L-serine | 0.8 | 11 | 0.069 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant K42A, O-acetyl-L-serine |
| cystathionine β-lyase (eCBL)variant S32A | Escherichia coli | cystathionine | 6.78 | 0.068 | 99 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant S32A, cystathionine |
| cystathionine β-lyase (eCBL)variant S32A | Escherichia coli | O-acetyl-L-serine | 0.71 | 4 | 0.178 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant S32A, O-acetyl-L-serine |
| cystathionine β-lyase (eCBL)variant S33A | Escherichia coli | cystathionine | 4.24 | 0.104 | 41 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant S33A, cystathionine |
| cystathionine β-lyase (eCBL)variant S33A | Escherichia coli | O-acetyl-L-serine | 0.076 | 1.08 | 0.07 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant S33A, O-acetyl-L-serine |