Values CatLog read from this paper
Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase, and Saccharomyces cerevisiae cystathionine gamma-lyase and…
PMID 19370061doi:10.1139/o08-144Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (63 values) from this paper: cystathionine γ-synthase (eCGS), cystathionine γ-lyase (yCGL), cystathionine β-lyase (eCBL) from Escherichia coli, Saccharomyces cerevisiae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
EC 2.5.1.48, EC 4.4.1.1, EC 4.4.1.13, EC 4.4.1.28
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cystathionine β-lyase (eCBL) | Escherichia coli | L-cystathionine | 35 | 0.23 | 152 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL), L-cystathionine |
| cystathionine β-lyase (eCBL) | Escherichia coli | L-cystathionine | 23 | 0.18 | 127 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL), L-cystathionine |
| cystathionine β-lyase (eCBL)variant F55D | Escherichia coli | L-cystathionine | 24 | 14 | 1.7 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant F55D, L-cystathionine |
| cystathionine β-lyase (eCBL)variant F55D/Y338E | Escherichia coli | L-cystathionine | — | — | 0.0022 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant F55D/Y338E, L-cystathionine |
| cystathionine β-lyase (eCBL)variant Y338E | Escherichia coli | L-cystathionine | — | — | 0.0445 | pH 8.5, 25 °C | Open record: cystathionine β-lyase (eCBL) variant Y338E, L-cystathionine |
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | L-cystathionine | 0.9 | 0.45 | 1.98 | pH 7.8, 25 °C | Open record: cystathionine γ-lyase (yCGL), L-cystathionine |
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | L-cystathionine | 1.81 | 0.42 | 4.3 | pH 7.8, 25 °C | Open record: cystathionine γ-lyase (yCGL), L-cystathionine |
| cystathionine γ-lyase (yCGL) | Saccharomyces cerevisiae | O-succinyl-L-homoserine | 0.12 | 17 | 0.007 | pH 7.8, 25 °C | Open record: cystathionine γ-lyase (yCGL), O-succinyl-L-homoserine |
| cystathionine γ-lyase (yCGL)variant E333A | Saccharomyces cerevisiae | L-cystathionine | 0.69 | 5.2 | 0.132 | pH 7.8, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333A, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E333Y | Saccharomyces cerevisiae | L-cystathionine | — | — | 0.147 | pH 7.8, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E333Y, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48A | Saccharomyces cerevisiae | L-cystathionine | 0.67 | 0.82 | 0.82 | pH 7.8, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48A, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48D | Saccharomyces cerevisiae | L-cystathionine | 0.76 | 1.11 | 0.68 | pH 7.8, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48D, L-cystathionine |
| cystathionine γ-lyase (yCGL)variant E48F | Saccharomyces cerevisiae | L-cystathionine | 0.398 | 0.81 | 0.49 | pH 7.8, 25 °C | Open record: cystathionine γ-lyase (yCGL) variant E48F, L-cystathionine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cystathionine | 0.043 | 3 | 0.013 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), L-cystathionine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteine | 91 | 0.43 | 320 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineγ-elimination (hydrolysis) of L-OSHS | 2.02 | 0.64 | 3.2 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys to L-Cth) | — | 3 | 30 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45F | Escherichia coli | L-cysteine | 5.6 | 2.4 | 2 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45F, L-cysteine |
| cystathionine γ-synthase (eCGS)variant D45F | Escherichia coli | O-succinyl-L-homoserineγ-elimination (hydrolysis) of L-OSHS | 1.38 | 2 | 0.69 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45F | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys to L-Cth) | — | 5.4 | 3 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45F/E325Y | Escherichia coli | O-succinyl-L-homoserine | 1.23 | 1.5 | 0.8 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45F/E325Y, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325Y | Escherichia coli | L-cysteine | 11.1 | 0.26 | 41 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325Y, L-cysteine |
| cystathionine γ-synthase (eCGS)variant E325Y | Escherichia coli | O-succinyl-L-homoserineγ-elimination (hydrolysis) of L-OSHS | 1.39 | 1.7 | 0.8 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325Y, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325Y | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys to L-Cth) | — | 2.1 | 7 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325Y, O-succinyl-L-homoserine |