EC 2.5.1.48: cystathionine gamma-synthase
TransferasesOpen in Browse
CatLog read 67 records for EC 2.5.1.48 from 3 papers, for 1 organism. Names in these papers: cystathionine γ-synthase (eCGS).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 21 records for EC 2.5.1.48 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.5.1.48 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Exploration of the active site of Escherichia coli cystathionine γ-synthase | Escherichia coli | 46 |
| Interconversion of a pair of active-site residues in Escherichia coli cystathionine gamma-synthase, E. coli cystathionine beta-lyase, and Saccharomyces cerevisiae cystathionine gamma-lyase and… | Escherichia coli | 11 |
| Escherichia coli cystathionine gamma-synthase does not obey ping-pong kinetics. Novel continuous assays for the elimination and substitution reactions | Escherichia coli | 10 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cystathionine | 0.043 | 3 | 0.013 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS), L-cystathionine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cystathionine | — | — | 0.0233 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS), L-cystathionine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteinemodified ping-pong mechanism (eqs 7-9) | — | 0.11 | 1.06 × 10³ | pH 7.8, 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteineordered mechanism (eq 10) | — | 0.1 | 1.2 × 10³ | pH 7.8, 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteine | 72 | 0.088 | 820 calc. | 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteine | 91 | 0.43 | 320 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteine | — | 0.24 | 470 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserine0.023-1.35 mM L-Cys; 0.1-22.5 mM L-OSHS; 0.025-0.25 µM eCGS | 121 | 2.5 | 49 | pH 7.8, 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserine0.023-1.35 mM L-Cys; 0.1-22.5 mM L-OSHS; 0.025-0.25 µM eCGS | 117 | 1.2 | 98 | pH 7.8, 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserine0.1-10 mM L-OSHS; 9.3 µM eCGS; no L-Cys; kcat per subunit | 1.8 | 1.3 | 1.35 | pH 7.8, 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineno L-Cys; coupled assay: HO-HxoDH with NADH | 5.49 | 0.29 | 12 | 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserine0.023-1.35 mM L-Cys; 0.1-22.5 mM L-OSHS; 0.025-0.25 µM eCGS | 3 | 4 | 0.75 calc. | pH 7.8, 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserinepH-dependence study; pH 6.1-9.0; 0.1-20 mM L-OSHS; 20 µM PLP | 2.66 | — | 6.3 | 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserinepH-dependence study; pH 6.6-9.4; 0.625-21.2 mM L-OSHS | 166 | — | 38 | 25 °C | PMID 14503880 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineγ-elimination (hydrolysis) of L-OSHS | 2.02 | 0.64 | 3.2 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys to L-Cth) | — | 3 | 30 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 112 | 4.4 | 25 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 3.65 | 1.9 | 2 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45A | Escherichia coli | L-cysteine | — | 0.8 | 99 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant D45A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant D45A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 79 | 19 | 4.2 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant D45A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.61 | 3.7 | 0.43 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant D45A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45F | Escherichia coli | L-cysteine | 5.6 | 2.4 | 2 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS) variant D45F, L-cysteine |
| cystathionine γ-synthase (eCGS)variant D45F | Escherichia coli | O-succinyl-L-homoserineγ-elimination (hydrolysis) of L-OSHS | 1.38 | 2 | 0.69 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS) variant D45F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45F | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys to L-Cth) | — | 5.4 | 3 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS) variant D45F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45F/E325Y | Escherichia coli | O-succinyl-L-homoserine | 1.23 | 1.5 | 0.8 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS) variant D45F/E325Y, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45N | Escherichia coli | L-cysteine | — | 0.9 | 113 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant D45N, L-cysteine |
| cystathionine γ-synthase (eCGS)variant D45N | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 100 | 39 | 2.6 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant D45N, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45N | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 2.64 | 4.6 | 0.58 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant D45N, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325A | Escherichia coli | L-cysteine | — | 0.17 | 350 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant E325A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant E325A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 59 | 0.7 | 90 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant E325A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 2.4 | 1.5 | 1.6 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant E325A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325Q | Escherichia coli | L-cysteine | — | 0.29 | 200 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant E325Q, L-cysteine |
| cystathionine γ-synthase (eCGS)variant E325Q | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 57 | 1.1 | 50 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant E325Q, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325Q | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 2.5 | 2 | 1.2 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant E325Q, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325Y | Escherichia coli | L-cysteine | 11.1 | 0.26 | 41 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS) variant E325Y, L-cysteine |
| cystathionine γ-synthase (eCGS)variant E325Y | Escherichia coli | O-succinyl-L-homoserineγ-elimination (hydrolysis) of L-OSHS | 1.39 | 1.7 | 0.8 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS) variant E325Y, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325Y | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys to L-Cth) | — | 2.1 | 7 | pH 7.8, 25 °C | PMID 19370061 | Open record: cystathionine γ-synthase (eCGS) variant E325Y, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant N227A | Escherichia coli | L-cysteine | — | 0.3 | 110 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant N227A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant N227A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 33 | 8 | 4.2 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant N227A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant N227A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 5.76 | 7.3 | 0.79 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant N227A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R106A | Escherichia coli | L-cysteine | — | 3 | 0.41 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R106A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R106A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 1.4 | 11 | 0.12 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R106A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R106A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 0.5 | 25 | 0.0199 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R106A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R106K | Escherichia coli | L-cysteine | — | 0.65 | 8.2 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R106K, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R106K | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 5.3 | 39 | 0.14 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R106K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R106K | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 0.99 | 20 | 0.051 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R106K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R361K | Escherichia coli | L-cysteine | 0.5 | 0.22 | 2.3 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R361K, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R361K | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | — | — | 0.0054 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R361K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R361K | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.6 | 240 | 0.0065 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R361K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R48K | Escherichia coli | L-cysteine | 0.15 | 0.22 | 0.68 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R48K, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R48K | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | — | — | 0.0021 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R48K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R48K | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 0.7 | 230 | 0.00263 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R48K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R49A | Escherichia coli | L-cysteine | — | 0.12 | 50 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R49A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R49A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 5.6 | 1.8 | 3.1 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R49A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R49A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.6 | 8.2 | 0.196 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R49A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R49K | Escherichia coli | L-cysteine | — | 0.36 | 360 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R49K, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R49K | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 130 | 8 | 16 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R49K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R49K | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 2.99 | 1.5 | 2 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant R49K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant S326A | Escherichia coli | L-cysteine | — | 0.2 | 1.6 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant S326A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant S326A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 0.31 | 21 | 0.015 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant S326A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant S326A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 0.093 | 9.3 | 0.01 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant S326A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant Y101F | Escherichia coli | L-cysteine | — | 0.15 | 64 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant Y101F, L-cysteine |
| cystathionine γ-synthase (eCGS)variant Y101F | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 9.6 | 1.9 | 5.1 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant Y101F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant Y101F | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.74 | 6.3 | 0.28 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant Y101F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant Y46F | Escherichia coli | L-cysteine | — | 0.6 | 11.3 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant Y46F, L-cysteine |
| cystathionine γ-synthase (eCGS)variant Y46F | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 6 | 90 | 0.071 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant Y46F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant Y46F | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.8 | 23 | 0.078 | pH 7.8, 25 °C | PMID 22855027 | Open record: cystathionine γ-synthase (eCGS) variant Y46F, O-succinyl-L-homoserine |