Values CatLog read from this paper
Exploration of the active site of Escherichia coli cystathionine γ-synthase
PMID 22855027doi:10.1002/pro.2135Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 46 records (119 values) from this paper: cystathionine γ-synthase (eCGS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cystathionine | — | — | 0.0233 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), L-cystathionine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteine | — | 0.24 | 470 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 112 | 4.4 | 25 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 3.65 | 1.9 | 2 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45A | Escherichia coli | L-cysteine | — | 0.8 | 99 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant D45A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 79 | 19 | 4.2 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.61 | 3.7 | 0.43 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45N | Escherichia coli | L-cysteine | — | 0.9 | 113 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45N, L-cysteine |
| cystathionine γ-synthase (eCGS)variant D45N | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 100 | 39 | 2.6 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45N, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant D45N | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 2.64 | 4.6 | 0.58 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant D45N, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325A | Escherichia coli | L-cysteine | — | 0.17 | 350 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant E325A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 59 | 0.7 | 90 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 2.4 | 1.5 | 1.6 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325Q | Escherichia coli | L-cysteine | — | 0.29 | 200 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325Q, L-cysteine |
| cystathionine γ-synthase (eCGS)variant E325Q | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 57 | 1.1 | 50 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325Q, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant E325Q | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 2.5 | 2 | 1.2 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant E325Q, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant N227A | Escherichia coli | L-cysteine | — | 0.3 | 110 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant N227A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant N227A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 33 | 8 | 4.2 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant N227A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant N227A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 5.76 | 7.3 | 0.79 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant N227A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R106A | Escherichia coli | L-cysteine | — | 3 | 0.41 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R106A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R106A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 1.4 | 11 | 0.12 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R106A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R106A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 0.5 | 25 | 0.0199 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R106A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R106K | Escherichia coli | L-cysteine | — | 0.65 | 8.2 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R106K, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R106K | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 5.3 | 39 | 0.14 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R106K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R106K | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 0.99 | 20 | 0.051 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R106K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R361K | Escherichia coli | L-cysteine | 0.5 | 0.22 | 2.3 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R361K, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R361K | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | — | — | 0.0054 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R361K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R361K | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.6 | 240 | 0.0065 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R361K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R48K | Escherichia coli | L-cysteine | 0.15 | 0.22 | 0.68 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R48K, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R48K | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | — | — | 0.0021 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R48K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R48K | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 0.7 | 230 | 0.00263 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R48K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R49A | Escherichia coli | L-cysteine | — | 0.12 | 50 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R49A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R49A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 5.6 | 1.8 | 3.1 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R49A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R49A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.6 | 8.2 | 0.196 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R49A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R49K | Escherichia coli | L-cysteine | — | 0.36 | 360 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R49K, L-cysteine |
| cystathionine γ-synthase (eCGS)variant R49K | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 130 | 8 | 16 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R49K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant R49K | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 2.99 | 1.5 | 2 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant R49K, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant S326A | Escherichia coli | L-cysteine | — | 0.2 | 1.6 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant S326A, L-cysteine |
| cystathionine γ-synthase (eCGS)variant S326A | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 0.31 | 21 | 0.015 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant S326A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant S326A | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 0.093 | 9.3 | 0.01 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant S326A, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant Y101F | Escherichia coli | L-cysteine | — | 0.15 | 64 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant Y101F, L-cysteine |
| cystathionine γ-synthase (eCGS)variant Y101F | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 9.6 | 1.9 | 5.1 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant Y101F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant Y101F | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.74 | 6.3 | 0.28 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant Y101F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant Y46F | Escherichia coli | L-cysteine | — | 0.6 | 11.3 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant Y46F, L-cysteine |
| cystathionine γ-synthase (eCGS)variant Y46F | Escherichia coli | O-succinyl-L-homoserineγ-replacement (condensation of L-OSHS and L-Cys) | 6 | 90 | 0.071 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant Y46F, O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS)variant Y46F | Escherichia coli | O-succinyl-L-homoserineα,γ-elimination (hydrolysis) of L-OSHS; no L-Cys | 1.8 | 23 | 0.078 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS) variant Y46F, O-succinyl-L-homoserine |