Values CatLog read from this paper
Escherichia coli cystathionine gamma-synthase does not obey ping-pong kinetics. Novel continuous assays for the elimination and substitution reactions
PMID 14503880doi:10.1021/bi035107oOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (28 values) from this paper: cystathionine γ-synthase (eCGS), L-lactate dehydrogenase (LDH), cystathionine β-lyase (eCBL) and 1 more from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
EC 1.1.1.27, EC 2.5.1.48, EC 4.4.1.3
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cystathionine β-lyase (eCBL) | Escherichia coli | L-cystathionine | — | — | 130 | pH 7.8, 25 °C | Open record: cystathionine β-lyase (eCBL), L-cystathionine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteinemodified ping-pong mechanism (eqs 7-9) | — | 0.11 | 1.06 × 10³ | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteineordered mechanism (eq 10) | — | 0.1 | 1.2 × 10³ | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | L-cysteine | 72 | 0.088 | 820 calc. | 25 °C | Open record: cystathionine γ-synthase (eCGS), L-cysteine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserine0.023-1.35 mM L-Cys; 0.1-22.5 mM L-OSHS; 0.025-0.25 µM eCGS | 121 | 2.5 | 49 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserine0.023-1.35 mM L-Cys; 0.1-22.5 mM L-OSHS; 0.025-0.25 µM eCGS | 117 | 1.2 | 98 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserine0.1-10 mM L-OSHS; 9.3 µM eCGS; no L-Cys; kcat per subunit | 1.8 | 1.3 | 1.35 | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserineno L-Cys; coupled assay: HO-HxoDH with NADH | 5.49 | 0.29 | 12 | 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserine0.023-1.35 mM L-Cys; 0.1-22.5 mM L-OSHS; 0.025-0.25 µM eCGS | 3 | 4 | 0.75 calc. | pH 7.8, 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserinepH-dependence study; pH 6.1-9.0; 0.1-20 mM L-OSHS; 20 µM PLP | 2.66 | — | 6.3 | 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| cystathionine γ-synthase (eCGS) | Escherichia coli | O-succinyl-L-homoserinepH-dependence study; pH 6.6-9.4; 0.625-21.2 mM L-OSHS | 166 | — | 38 | 25 °C | Open record: cystathionine γ-synthase (eCGS), O-succinyl-L-homoserine |
| D-2-hydroxyisocaproate dehydrogenase (HO-HxoDH) | — | 2-oxobutanoate | — | — | 16 | pH 7.8, 25 °C | Open record: D-2-hydroxyisocaproate dehydrogenase (HO-HxoDH), 2-oxobutanoate |
| L-lactate dehydrogenase (LDH) | — | 2-oxobutanoate | — | — | 1.8 | pH 7.8, 25 °C | Open record: L-lactate dehydrogenase (LDH), 2-oxobutanoate |
| L-lactate dehydrogenase (LDH) | — | pyruvate | — | — | 110 | pH 7.8, 25 °C | Open record: L-lactate dehydrogenase (LDH), pyruvate |