Values CatLog read from this paper
Mechanism and inhibition of the FabV enoyl-ACP reductase from Burkholderia mallei
PMID 20055482doi:10.1021/bi902001aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (35 values) from this paper: FabV enoyl-ACP reductase from Burkholderia mallei.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| FabV enoyl-ACP reductase | Burkholderia mallei | crotonyl-acyl carrier protein (ftuACP from Francisella tularensis SCHU S4 acylated with crotonic anhydride) | 21.65 | 0.0251 | 863 calc. | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase, crotonyl-acyl carrier protein (ftuACP from Francisella tularensis SCHU S4 acylated with crotonic anhydride) |
| FabV enoyl-ACP reductase | Burkholderia mallei | reduced nicotinamide adenine dinucleotideNADH oxidation followed at 340 nm; cosubstrate DD-CoA | — | 0.023 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductase | Burkholderia mallei | reduced nicotinamide adenine dinucleotideNADH oxidation followed at 340 nm; cosubstrate DD-CoA | — | 0.0287 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductase | Burkholderia mallei | reduced nicotinamide adenine dinucleotidecosubstrate crotonyl-ACP (Cr-ACP); in the text | — | 0.031 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductase | Burkholderia mallei | trans-2-dodecenoyl-CoAN-terminal His tag removed with thrombin; Table 2 | 28.8 | 0.0044 | 6.53 × 10³ | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase, trans-2-dodecenoyl-CoA |
| FabV enoyl-ACP reductase | Burkholderia mallei | trans-2-dodecenoyl-CoAN-terminal His tag; Table 2 | 20.7 | 0.0025 | 8.28 × 10³ | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase, trans-2-dodecenoyl-CoA |
| FabV enoyl-ACP reductase | Burkholderia mallei | trans-2-dodecenoyl-CoAN-terminal His tag; in the text | 15.5 | 0.0024 | 6.5 × 10³ calc. | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase, trans-2-dodecenoyl-CoA |
| FabV enoyl-ACP reductasevariant K244A | Burkholderia mallei | reduced nicotinamide adenine dinucleotide | — | 0.066 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant K244A, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductasevariant K244A | Burkholderia mallei | trans-2-dodecenoyl-CoA | 0.18 | 0.0072 | 25 | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant K244A, trans-2-dodecenoyl-CoA |
| FabV enoyl-ACP reductasevariant K244A/K245A | Burkholderia mallei | reduced nicotinamide adenine dinucleotide | — | 0.065 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant K244A/K245A, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductasevariant K244A/K245A | Burkholderia mallei | trans-2-dodecenoyl-CoA | — | — | 0.38 | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant K244A/K245A, trans-2-dodecenoyl-CoA |
| FabV enoyl-ACP reductasevariant K244R | Burkholderia mallei | reduced nicotinamide adenine dinucleotide | — | 0.069 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant K244R, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductasevariant K244R | Burkholderia mallei | trans-2-dodecenoyl-CoA | 0.022 | 0.0076 | 2.8 | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant K244R, trans-2-dodecenoyl-CoA |
| FabV enoyl-ACP reductasevariant K245M | Burkholderia mallei | reduced nicotinamide adenine dinucleotide | — | 0.028 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant K245M, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductasevariant K245M | Burkholderia mallei | trans-2-dodecenoyl-CoA | 0.3 | 0.028 | 10.7 | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant K245M, trans-2-dodecenoyl-CoA |
| FabV enoyl-ACP reductasevariant Y235A | Burkholderia mallei | reduced nicotinamide adenine dinucleotide | — | 0.023 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant Y235A, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductasevariant Y235A | Burkholderia mallei | trans-2-dodecenoyl-CoA | 0.083 | 0.0027 | 30 | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant Y235A, trans-2-dodecenoyl-CoA |
| FabV enoyl-ACP reductasevariant Y235S | Burkholderia mallei | reduced nicotinamide adenine dinucleotide | — | 0.006 | — | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant Y235S, reduced nicotinamide adenine dinucleotide |
| FabV enoyl-ACP reductasevariant Y235S | Burkholderia mallei | trans-2-dodecenoyl-CoA | 0.102 | 0.0022 | 47 | pH 7.9, 25 °C | Open record: FabV enoyl-ACP reductase variant Y235S, trans-2-dodecenoyl-CoA |