Values CatLog read from this paper
Mutational analysis of parasite trypanothione reductase: acquisition of glutathione reductase activity in a triple mutant
PMID 2007114doi:10.1021/bi00225a004Open in Browse
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CatLog read 16 records (41 values) from this paper: trypanothione reductase from Trypanosoma congolense.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| trypanothione reductase | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 88 | 0.025 | 3.53 × 10³ | pH 7.8, 25 °C | Open record: trypanothione reductase, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductase | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 152 | 0.018 | 8.42 × 10³ | pH 6.6, 25 °C | Open record: trypanothione reductase, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant A343R | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 28 | 0.009 | 3.13 × 10³ | pH 6.6, 25 °C | Open record: trypanothione reductase variant A343R, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant E18A | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 63 | 0.266 | 230 | pH 6.6, 25 °C | Open record: trypanothione reductase variant E18A, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant E18A/A343R | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 8.25 | 0.375 | 22 | pH 6.6, 25 °C | Open record: trypanothione reductase variant E18A/A343R, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant E18A/W21R | Trypanosoma congolense | glutathione disulfide | — | — | 0.073 | pH 6.6, 25 °C | Open record: trypanothione reductase variant E18A/W21R, glutathione disulfide |
| trypanothione reductasevariant E18A/W21R | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 50 | 5.2 | 9.5 | pH 6.6, 25 °C | Open record: trypanothione reductase variant E18A/W21R, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant E18A/W21R/A343R | Trypanosoma congolense | glutathione disulfideTable III | — | 70 | 0.23 | pH 6.6, 25 °C | Open record: trypanothione reductase variant E18A/W21R/A343R, glutathione disulfide |
| trypanothione reductasevariant E18A/W21R/A343R | Trypanosoma congolense | glutathione disulfidein the text | 16.3 | — | — | pH 6.6, 25 °C | Open record: trypanothione reductase variant E18A/W21R/A343R, glutathione disulfide |
| trypanothione reductasevariant E18A/W21R/A343R | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 30 | 4.5 | 6.7 | pH 6.6, 25 °C | Open record: trypanothione reductase variant E18A/W21R/A343R, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant K483* | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 57 | 0.011 | 5.15 × 10³ | pH 7.8, 25 °C | Open record: trypanothione reductase variant K483*, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant P488* | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 67 | 0.022 | 3.07 × 10³ | pH 7.8, 25 °C | Open record: trypanothione reductase variant P488*, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant T473* | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 88 | 0.012 | 5.2 × 10³ | pH 7.8, 25 °C | Open record: trypanothione reductase variant T473*, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant W21R | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 35 | 0.6 | 52 | pH 6.6, 25 °C | Open record: trypanothione reductase variant W21R, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant W21R/A343R | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | 70 | 2.7 | 25 | pH 6.6, 25 °C | Open record: trypanothione reductase variant W21R/A343R, N1,N8-bis(glutathionyl)spermidine disulfide |
| trypanothione reductasevariant Y478* | Trypanosoma congolense | N1,N8-bis(glutathionyl)spermidine disulfide | — | 0.033 | — | pH 7.8, 25 °C | Open record: trypanothione reductase variant Y478*, N1,N8-bis(glutathionyl)spermidine disulfide |