Values CatLog read from this paper
Studies on a Tyr residue critical for the binding of coenzyme and substrate in mouse 3(17)alpha-hydroxysteroid dehydrogenase (AKR1C21): structure of the Y224D mutant enzyme
PMID 20124700doi:10.1107/s0907444909051464Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 36 records (100 values) from this paper: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) from Mus musculus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) | Mus musculus | NADP+ | 0.063 | 5 × 10⁻⁴ | 130 calc. | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21), NADP+ |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) | Mus musculus | NADPH | 0.38 | 5 × 10⁻⁴ | 770 calc. | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21), NADPH |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | 4-Androstene-3,17-dione | 0.62 | 0.0059 | 105 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, 4-Androstene-3,17-dione |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | 5-Androstene-3β,17β-diol | 0.065 | 0.1 | 0.65 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, 5-Androstene-3β,17β-diol |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | 5α-Androstan-17α-ol-3-one | 0.28 | 0.014 | 20 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, 5α-Androstan-17α-ol-3-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | 5α-Androstan-17β-ol-3-one | 0.037 | 0.082 | 0.45 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, 5α-Androstan-17β-ol-3-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | 5α-Androstan-3α-ol-17-one | 0.23 | 0.0034 | 68 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, 5α-Androstan-3α-ol-17-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | 5β-Pregnan-20α-ol-3-one | 2.83 | 7 × 10⁻⁴ | 4 × 10³ | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, 5β-Pregnan-20α-ol-3-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | 5β-Pregnane-3,20-dione | 4.8 | 4 × 10⁻⁴ | 1.2 × 10⁴ | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, 5β-Pregnane-3,20-dione |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | 5β-Pregnane-3α,20α-diol | 0.25 | 0.0038 | 65 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, 5β-Pregnane-3α,20α-diol |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | epitestosterone | 0.23 | 0.02 | 11.7 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, epitestosterone |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | Lithocholic acid | 0.105 | 6 × 10⁻⁴ | 180 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, Lithocholic acid |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | NADP+ | 0.132 | 0.015 | 8.8 calc. | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, NADP+ |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Q222N | Mus musculus | NADPH | 5.17 | 0.005 | 1.0 × 10³ calc. | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Q222N, NADPH |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | 4-Androstene-3,17-dione | 0.022 | 0.0078 | 3.3 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, 4-Androstene-3,17-dione |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | 5-Androstene-3β,17β-diol | 0.0017 | 0.035 | 0.05 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, 5-Androstene-3β,17β-diol |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | 5α-Androstan-17β-ol-3-one | 0.0025 | 0.096 | 0.033 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, 5α-Androstan-17β-ol-3-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | 5α-Androstan-3α-ol-17-one | 0.022 | 0.015 | 1.5 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, 5α-Androstan-3α-ol-17-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | 5β-Pregnan-20α-ol-3-one | 1.25 | 0.015 | 83 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, 5β-Pregnan-20α-ol-3-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | 5β-Pregnane-3,20-dione | 0.35 | 0.0059 | 60 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, 5β-Pregnane-3,20-dione |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | 5β-Pregnane-3α,20α-diol | 0.0037 | 0.018 | 1.7 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, 5β-Pregnane-3α,20α-diol |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | Lithocholic acid | 0.0073 | 0.0067 | 1.2 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, Lithocholic acid |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | NADP+ | 0.0048 | 0.04 | 0.12 calc. | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, NADP+ |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224D | Mus musculus | NADPH | 0.35 | 0.024 | 15 calc. | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224D, NADPH |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | 4-Androstene-3,17-dione | 0.32 | 0.0015 | 220 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, 4-Androstene-3,17-dione |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | 5-Androstene-3β,17β-diol | 0.0022 | 0.013 | 0.17 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, 5-Androstene-3β,17β-diol |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | 5α-Androstan-17α-ol-3-one | 0.87 | 0.0054 | 160 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, 5α-Androstan-17α-ol-3-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | 5α-Androstan-17β-ol-3-one | 0.0027 | 0.0047 | 0.5 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, 5α-Androstan-17β-ol-3-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | 5α-Androstan-3α-ol-17-one | 0.127 | 0.0018 | 70 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, 5α-Androstan-3α-ol-17-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | 5β-Pregnan-20α-ol-3-one | 4.83 | 6 × 10⁻⁴ | 8 × 10³ | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, 5β-Pregnan-20α-ol-3-one |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | 5β-Pregnane-3,20-dione | 4 | 5 × 10⁻⁴ | 8 × 10³ | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, 5β-Pregnane-3,20-dione |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | 5β-Pregnane-3α,20α-diol | 0.4 | 0.0032 | 125 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, 5β-Pregnane-3α,20α-diol |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | epitestosterone | 0.57 | 0.014 | 40 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, epitestosterone |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | Lithocholic acid | 0.082 | 2 × 10⁻⁴ | 400 | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, Lithocholic acid |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | NADP+ | 0.092 | 0.0082 | 11 calc. | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, NADP+ |
| 3(17)α-hydroxysteroid dehydrogenase (AKR1C21)variant Y224F | Mus musculus | NADPH | 4.17 | 0.0034 | 1.2 × 10³ calc. | pH 7, 25 °C | Open record: 3(17)α-hydroxysteroid dehydrogenase (AKR1C21) variant Y224F, NADPH |