CatLogenzyme kinetics catalog

Values CatLog read from this paper

Beta sheet 2-alpha helix C loop of cytochrome P450 reductase serves as a docking site for redox partners

PMID 20152939doi:10.1016/j.bbapap.2010.02.003Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (90 values) from this paper: NADPH-cytochrome P450 reductase (CPR) from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.6.2.4

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 10 mM5.80.00222.7 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 30 mM8.50.00312.7 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 60 mM120.00393 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 110 mM180.00623 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 210 mM280.012.8 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 30 mM170.0028.3 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 60 mM14.70.0043.7 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 110 mM200.00852.3 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 210 mM330.021.7 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56QRattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 310 mM350.0231.52 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 10 mM10.50.00215 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 30 mM13.80.00265.3 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 60 mM200.00414.8 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 110 mM300.00634.8 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 210 mM400.00874.7 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 30 mM250.00251 × 10⁴pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 60 mM230.00524.5 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 110 mM370.0113.3 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 210 mM650.0262.5 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/D113ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 310 mM550.0252.2 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 10 mM130.00187.2 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 30 mM180.00266.8 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 60 mM250.00386.5 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 110 mM380.00655.8 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicus3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideKCl added to give ionic strength 210 mM570.0115 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 30 mM250.00241.05 × 10⁴pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 60 mM250.00367 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 110 mM370.0066 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 210 mM580.0134.5 × 10³pH 7.5, 25 °COpen
NADPH-cytochrome P450 reductase (CPR)variant K56Q/E115A/E116ARattus norvegicuscytochrome c (horse heart), oxidized form (cyt c^{3+})KCl added to give ionic strength 310 mM580.0163.7 × 10³pH 7.5, 25 °COpen